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Mouse Endoglin/CD105 Antibody

Catalog #: MAB1320
14 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1320
MAB1320-SP
Endoglin/CD105 in Mouse Embryo.
4 Images
Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Endoglin/CD105 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Endoglin in direct ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant human Endoglin.
Source
Monoclonal Rat IgG2A Clone # 209701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endoglin
Glu21-Gly581 (predicted)
Accession # NP_031958
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Endoglin/CD105 Fc Chimera (Catalog # 1320-EN)
under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
MS‑1 mouse pancreatic islet endothelial cell line
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Endoglin/CD105 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Endoglin/CD105 in Mouse Embryo. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (13.5 d.p.c.) using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells of the developing hindlimb. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Image depicts staining at 10X magnification.

Immunohistochemistry Endoglin/CD105 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Endoglin/CD105 in Mouse Embryo. Endoglin/CD105 was detected in immersion fixed frozen sections of mouse embryo (13.5 d.p.c.) using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells of the developing hindlimb. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Image depicts staining at 40X magnification.

Immunocytochemistry Endoglin/CD105 antibody in Mouse Mesenchymal Stem Cells by Immunocytochemistry (ICC). View Larger

Endoglin/CD105 in Mouse Mesenchymal Stem Cells. Endoglin/CD105 was detected in immersion fixed mouse mesenchymal stem cells using Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow Cytometry View Larger

Detection of Endoglin/CD105 in MS-1 cells by Flow Cytometry MS-1 cells were stained with Rat Anti-Mouse Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% amino acid (aa) identity within the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal, and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Mouse Endoglin cDNA encodes 653 aa including a 26 aa signal sequence, a 555 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). A mouse isoform with a 35 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) Endoglin (6, 7). The mouse Endoglin ECD shares 69%, 84%, 62%, 63%, and 66% aa identity with human, rat, bovine, porcine, and canine Endoglin, respectively. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind Endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of Endoglin can inhibit TGF-beta 1 signals but enhance BMP-7 signals in the same myoblast cell line (11). In endothelial cells, Endoglin inhibits T beta RI/ALK5 but enhances ALK1-mediated activation (12). Deletion of mouse Endoglin causes lethal vascular and cardiovascular defects, and human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of Endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble Endoglin and VEGF R1 (sFlt-1), along with low placental growth factor (PlGF), are pathogenic due to anti-angiogenic activity (15).

References
  1. Ge, A.Z. and E.C. Butcher (1994) Gene 138:201.
  2. ten Dijke, P. et al. (2008) Angiogenesis 11:79.
  3. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375.
  4. Mancini, M.L. et al. (2007) Dev. Biol. 308:520.
  5. Moody, J.L. et al. (2007) Stem Cells 25:2809.
  6. Velasco, S. et al. (2008) J. Cell Sci. 121:913.
  7. Perez-Gomez, E. et al. (2005) Oncogene 24:4450.
  8. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027.
  9. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
  10. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964.
  11. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934.
  12. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800.
  13. Arthur, H.M. et al. (2000) Dev. Biol. 217:42.
  14. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25.    
  15. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs
2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names
CD105 antigen; CD105; Endoglin; ENDOsler-Rendu-Weber syndrome 1; ENG; HHT1FLJ41744; ORW; ORW1

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Citations for Mouse Endoglin/CD105 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Multilayered, Hyaluronic Acid-Based Hydrogel Formulations Suitable for Automated 3D High Throughput Drug Screening of Cancer-Stromal Cell Cocultures.
    Authors: Engel BJ, Constantinou PE, Sablatura LK et al.
    Adv Healthc Mater
  2. Release of STK24/25 suppression on MEKK3 signaling in endothelial cells confers Cerebral cavernous malformation
    Authors: X Yang, ST Wu, R Gao, R Wang, Y Wang, Z Dong, L Wang, C Qi, X Wang, ML Schmitz, R Liu, Z Han, L Wang, X Zheng
    JCI Insight, 2023-03-08;0(0):.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  3. Assessment of the association of OCT3/4 with GLUT1 and CD105 in oral squamous cell carcinoma using dual immunohistochemistry
    Authors: Samira Derakhshan, Nazanin Mahdavi, Neda Kardouni Khoozestani, Bita Nasr Esfahani, Foroozan Heidarian, Sedigheh Rahrotaban et al.
    BMC Oral Health
  4. Immature Vascular Smooth Muscle Cells in Healthy Murine Arteries and Atherosclerotic Plaques: Localization and Activity
    Authors: A Balatskiy, I Ozhimalov, M Balatskaya, A Savina, J Filatova, N Kalinina, V Popov, V Tkachuk
    International Journal of Molecular Sciences, 2022-02-03;23(3):.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Gli1+ Cells Couple with Type H Vessels and Are Required for Type H Vessel Formation
    Authors: J Chen, M Li, AQ Liu, CX Zheng, LH Bao, K Chen, XL Xu, JT Guan, M Bai, T Zhou, BD Sui, DH Li, Y Jin, CH Hu
    Stem Cell Reports, 2020-07-14;15(1):110-124.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Caspase-8 modulates physiological and pathological angiogenesis during retina development
    Authors: Nathalie Tisch, Aida Freire-Valls, Rosario Yerbes, Isidora Paredes, Silvia La Porta, Xiaohong Wang et al.
    Journal of Clinical Investigation
  7. Volume expansion and TRPV4 activation regulate stem cell fate in three-dimensional microenvironments
    Authors: HP Lee, R Stowers, O Chaudhuri
    Nat Commun, 2019-01-31;10(1):529.
    Species: Mouse
    Sample Types: Hydrogel Cells
    Applications: IHC-Fr
  8. Gli1(+) Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target
    Authors: RK Schneider, A Mullally, A Dugourd, F Peisker, R Hoogenboez, PMH Van Strien, EM Bindels, D Heckl, G Büsche, D Fleck, G Müller-New, J Wongboonsi, M Ventura Fe, VG Puelles, J Saez-Rodri, BL Ebert, BD Humphreys, R Kramann
    Cell Stem Cell, 2017-04-27;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Bistable Epigenetic States Explain Age-Dependent Decline in Mesenchymal Stem Cell Heterogeneity
    Authors: Z Hamidouche, K Rother, J Przybilla, A Krinner, D Clay, L Hopp, C Fabian, A Stolzing, H Binder, P Charbord, J Galle
    Stem Cells, 2016-11-08;35(3):694-704.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Hiding inside? Intracellular expression of non-glycosylated c-kit protein in cardiac progenitor cells
    Authors: Huilin Shi, Christopher A. Drummond, Xiaoming Fan, Steven T. Haller, Jiang Liu, Deepak Malhotra et al.
    Stem Cell Research
  11. Retinal angiogenesis in the Ins2(Akita) mouse model of diabetic retinopathy
    Authors: Zongchao Han, Junjing Guo, Shannon M. Conley, Muna I. Naash
    Investigative Opthalmology & Visual Science
  12. Mesenchymal stem cells originating from ES cells show high telomerase activity and therapeutic benefits.
    Authors: Ninagawa N, Murakami R, Isobe E, Tanaka Y, Nakagawa H, Torihashi S
    Differentiation, 2011-08-19;82(3):153-64.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Selection, ICC
  13. PIR-B-deficient mice are susceptible to Salmonella infection.
    Authors: Torii I, Oka S, Hotomi M, Benjamin WH, Takai T, Kearney JF, Briles DE, Kubagawa H
    J. Immunol., 2008-09-15;181(6):4229-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. sFRP2 Supersedes VEGF as an Age-related Driver of Angiogenesis in Melanoma, Affecting Response to Anti-VEGF Therapy in Older Patients.
    Authors: Fane, M E, Ecker, B L Et al.
    Clin Cancer Res

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Reviews for Mouse Endoglin/CD105 Antibody

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Mouse Endoglin/CD105 Antibody
By Anonymous on 10/06/2021
Application: IHC Sample Tested: Hepatocellular carcinoma Species: Human
Immunohistochemistry Mouse Endoglin/CD105 Antibody MAB1320
Mouse Endoglin/CD105 Antibody
By Frederic Thalheimer on 08/01/2016
Application: IHC Sample Tested: Mouse Brain slices fixed with 4% PFA solution Species: Mus Musculus

Endoglin/CD105 was detected 4% PFA fixed mouse brain. Brain sections were cutted with an Vibratom. Endoglin detection was performed with Endoglin/CD105 Monoclonal Antibody (Catalog # MAB1320) at 20 µg/mL overnight at 4 °C. Brain sections were afterwards stained with AlexaFluor 647 coupled Anti-Rat IgG Secondary Antibody and counterstained with Hoechst 33342 (blue). Specific staining was localized to endothelial cells in Striatum of mouse brain. Transduced Neurons Express GFP.

Immunohistochemistry Mouse Endoglin/CD105 Antibody MAB1320