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Mouse Fc gamma RIII (CD16) Antibody

Catalog #: AF1960
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1960
AF1960-SP
Fc gamma RIII (CD16) in Mouse Splenocytes.
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Citations (4)
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Mouse Fc gamma RIII (CD16) Antibody Summary

Species Reactivity
Mouse
Specificity
Detects Fc gamma RIIIA/B (CD16) in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Fc gamma RIIIB and rhCD32 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 µg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Fc gamma  RIIIA/B (CD16) (Catalog # 1960-FC)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.1-0.3 µg/mL of this antibody will block 50% of the binding of 200 ng/mL of mouse IgG to immobilized Recombinant Mouse Fc gamma  RIII/CD16 (Catalog # 1960-FC) coated at 2 µg/mL (100 µL/well). At 2 μg/mL, this antibody will block >90% of the binding.
 
Immunocytochemistry
5-15 µg/mL
Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Fc gamma RIII (CD16) in Mouse Splenocytes. Fc gamma RIII (CD16) was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Fc gamma RIII (CD16) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1960) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: Fc gamma RIII (CD16)

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10‑8‑10‑9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Mouse CD16 is encoded by a single gene. The protein product is a type I transmembrane protein having two extracellular Ig-like domains. It is expressed on a variety of myeloid and lymphoid cells (4) and associates with Fc R gamma to deliver an activating signal upon ligand binding (5). Mouse CD32 is closely related to mouse CD16 throughout its extracellular domain (95% amino acid sequence identity), but has a divergent cytoplasmic domain and functions as an inhibitory receptor. Together these proteins constitute an activating/inhibiting receptor pair to regulate immune responses (5).

References
  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
Long Name
Fc gamma Receptor III
Entrez Gene IDs
14131 (Mouse); 102140945 (Cynomolgus Monkey)
Alternate Names
CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20

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Citations for Mouse Fc gamma RIII (CD16) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Immunoglobulin directly enhances differentiation of oligodendrocyte-precursor cells and remyelination
    Authors: Li, Y;Noto, D;Hoshino, Y;Mizuno, M;Yoshikawa, S;Miyake, S;
    Scientific reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Fc gamma receptor activation mediates vascular inflammation and abdominal aortic aneurysm development
    Authors: Laura Lopez‐Sanz, Susana Bernal, Luna Jimenez‐Castilla, Ignacio Prieto, Sara La Manna, Sergio Gomez‐Lopez et al.
    Clinical and Translational Medicine
  3. Immune complexes stimulate CCR7-dependent dendritic cell migration to lymph nodes
    Authors: Menna R. Clatworthy, Caren E. Petrie Aronin, Rebeccah J. Mathews, Nicole Y Morgan, Kenneth G.C. Smith, Ronald N. Germain
    Nature Medicine
  4. Altered balance of inhibitory and active Fc gamma receptors in murine autoimmune glomerulonephritis.
    Authors: Ichii O, Konno A, Sasaki N, Endoh D, Hashimoto Y, Kon Y
    Kidney Int., 2008-05-07;74(3):339-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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