Mouse FGFR4 Biotinylated Antibody

Catalog #: BAF2265 Datasheet / COA / SDS

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BAF2265

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Mouse FGFR4 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse FGF R4 in Western blots. In Western blots, approximately 35% cross‑reactivity with recombinant human (rh) FGF R4 is observed and less than 5% cross-reactivity with rhFGF R1 alpha (IIIb), rhFGF R1 alpha (IIIc), rhFGF R1 beta (IIIb), rhFGF R1 beta (IIIc), recombinant mouse (rm) FGF R2 alpha (IIIb), rmFGF R2 alpha (IIIc), rmFGF R2 beta (IIIb), rmFGF R2 beta (IIIc), rmFGF R5, and rmFGF R5 beta is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FGF R4
Leu19-Asp366
Accession # CAA42551

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse FGF R4

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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FGF R4 in Mouse Embryo. FGF R4 was detected in immersion fixed frozen sections of mouse embryo (E11.5) using Goat Anti-Mouse FGF R4 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2265) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the epidermis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGFR4

FGF R4 is a transmembrane receptor tyrosine kinase that binds several ligands of the FGF family. It is expressed in the early embryo, and its expression has been suggested to play an important role in the formation of mesoderm and posterior structure (1). Recent studies also indicated that FGF R4 may be used as a marker to distinguish neural stem cells from other precursor cells (2). The extracellular domain of mouse FGF R4 shares 95% and 89% amino acid sequence homology with its rat and human homologs, respectively.

References

Umbhauer, M.J. et al. (2000) J. Cell Sci. 113:2865.
Cai, J. (2002) Dev. Biol. 251:221.

Long Name

Fibroblast Growth Factor Receptor 4

Entrez Gene IDs

2264 (Human); 14186 (Mouse)

Alternate Names

CD334 antigen; CD334; EC 2.7.10; EC 2.7.10.1; FGF R4; FGFR4; FGFR-4; fibroblast growth factor receptor 4; JTK2hydroxyaryl-protein kinase; MGC20292; protein-tyrosine kinase; TKF; tyrosine kinase related to fibroblast growth factor receptor; tyrosylprotein kinase