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Mouse IGF-I/IGF-1 Antibody

Catalog #: AF791
34 Citations 1 Review Datasheet / COA / SDS
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AF791
AF791-SP
Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody.
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Citations (34)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGF-I/IGF-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human IGF-I/IGF-1 is observed, and less than 2% cross-reactivity with recombinant mouse IGF-II/IGF-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IGF-I/IGF-1
Gl33-Ala102
Accession # Q8CAR0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IGF-I/IGF-1-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K. P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse IGF-I/IGF-1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody. View Larger

Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Mouse IGF-I/IGF-1 Antibody. Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND50 is typically 0.2-1.0 µg/mL.

Immunohistochemistry IGF-I/IGF-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

IGF-I/IGF-1 in Mouse Embryo. IGF-I/IGF-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Mouse IGF-I is synthesized as two precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature mouse IGF-I shares 94% and 99% aa sequence identity with human and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 60% aa sequence identity with mature mouse IGF‑II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects (1). IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4-7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

References
  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Bell, G.I. et al. (1986) Nucleic Acids Res. 14:7873.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C

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Citations for Mouse IGF-I/IGF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Protection of insulin‑like growth factor 1 on experimental peripheral neuropathy in diabetic mice
    Authors: Hua Wang, Hao Zhang, Fuming Cao, Jiaping Lu, Jin Tang, Huizhi Li et al.
    Molecular Medicine Reports
  2. Resident cardiac macrophages mediate adaptive myocardial remodeling
    Authors: Nicole R. Wong, Jay Mohan, Benjamin J. Kopecky, Shuchi Guo, Lixia Du, Jamison Leid et al.
    Immunity
  3. M2 microglia and macrophages drive oligodendrocyte differentiation during CNS remyelination
    Authors: Veronique E. Miron, Amanda Boyd, Jing-Wei Zhao, Tracy J. Yuen, Julia M. Ruckh, Jennifer L. Shadrach et al.
    Nature Neuroscience
  4. Stress Granules Determine the Development of Obesity-Associated Pancreatic Cancer
    Authors: Guillaume Fonteneau, Alexandra Redding, Hannah Hoag-Lee, Edward S. Sim, Stefan Heinrich, Matthias M. Gaida et al.
    Cancer Discovery
  5. Lacrimal gland budding requires PI3K-dependent suppression of EGF signaling
    Authors: Wang Q, Tao C, Hannan A et al.
    Science Advances
  6. Localized SCF and IGF-1 secretion enhances erythropoiesis in the spleen of murine embryos
    Authors: Keai Sinn Tan, Tomoko Inoue, Kasem Kulkeaw, Yuka Tanaka, Mei I Lai, Daisuke Sugiyama
    Biology Open
  7. Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in mice
    Authors: Sandovici, I;Fernandez-Twinn, DS;Campbell, N;Cooper, WN;Sekita, Y;Zvetkova, I;Ferland-McCollough, D;Prosser, HM;Oyama, LM;Pantaleão, LC;Cimadomo, D;Barbosa de Queiroz, K;Cheuk, CSK;Smith, NM;Kay, RG;Antrobus, R;Hoelle, K;Ma, MKL;Smith, NH;Geyer, SH;Reissig, LF;Weninger, WJ;Siddle, K;Willis, AE;Lam, BYH;Bushell, M;Ozanne, SE;Constância, M;
    Cell reports
    Species: Mouse, Transgenic Mouse
    Sample Types: Plasma, Tissue Homogenates
    Applications: Western Blot
  8. Myonectin protects against skeletal muscle dysfunction in male mice through activation of AMPK/PGC1? pathway
    Authors: Ozaki, Y;Ohashi, K;Otaka, N;Kawanishi, H;Takikawa, T;Fang, L;Takahara, K;Tatsumi, M;Ishihama, S;Takefuji, M;Kato, K;Shimizu, Y;Bando, YK;Inoue, A;Kuzuya, M;Miura, S;Murohara, T;Ouchi, N;
    Nature communications
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. Osteoclast-derived IGF1 induces RANKL production in osteocytes and contributes to Pagetic lesion formation
    Authors: Miyagawa, K;Tenshin, H;Mulcrone, PL;Delgado-Calle, J;Subler, MA;Windle, JJ;Chirgwin, JM;Roodman, GD;Kurihara, N;
    JCI insight
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Bone marrow-derived IGF-1 orchestrates maintenance and regeneration of the adult skeleton
    Authors: J Wang, Q Zhu, D Cao, Q Peng, X Zhang, C Li, C Zhang, BO Zhou, R Yue
    Proceedings of the National Academy of Sciences of the United States of America, 2022-12-28;120(1):e2203779120.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  11. Single-cell transcriptome analyses reveal microglia types associated with proliferative retinopathy
    Authors: Z Liu, H Shi, J Xu, Q Yang, Q Ma, X Mao, Z Xu, Y Zhou, Q Da, Y Cai, DJ Fulton, Z Dong, A Sodhi, RB Caldwell, Y Huo
    JCI Insight, 2022-12-08;7(23):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC/IF
  12. Microglia-neuron interaction at nodes of Ranvier depends on neuronal activity through potassium release and contributes to remyelination
    Authors: R Ronzano, T Roux, M Thetiot, MS Aigrot, L Richard, FX Lejeune, E Mazuir, JM Vallat, C Lubetzki, A Desmazière
    Nature Communications, 2021-09-01;12(1):5219.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Muscle insulin-like growth factor-I modulates murine craniofacial bone growth
    Authors: HJ Kok, CN Crowder, L Koo Min Ch, HY Choi, N Lin, ER Barton
    European cells & materials, 2021-07-19;41(0):72-89.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Lacrimal gland budding requires PI3K-dependent suppression of EGF signaling
    Authors: Wang Q, Tao C, Hannan A et al.
    Science Advances
  15. Forceful mastication activates osteocytes and builds a stout jawbone
    Authors: M Inoue, T Ono, Y Kameo, F Sasaki, T Ono, T Adachi, T Nakashima
    Sci Rep, 2019-03-20;9(1):4404.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC-P, Neutralization
  16. Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury
    Authors: V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix
    Nat Commun, 2019-01-31;10(1):518.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Obesity-Activated Adipose-Derived Stromal Cells Promote Breast Cancer Growth and Invasion
    Authors: LE Hillers, JV D'Amato, T Chamberlin, G Paderta, LM Arendt
    Neoplasia, 2018-10-12;20(11):1161-1174.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  18. AHNAK Loss in Mice Promotes Type II Pneumocyte Hyperplasia and Lung Tumor Development
    Authors: JW Park, IY Kim, JW Choi, HJ Lim, JH Shin, YN Kim, SH Lee, Y Son, M Sohn, JK Woo, JH Jeong, C Lee, YS Bae, JK Seong
    Mol. Cancer Res., 2018-05-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Micorarray
    Applications: IHC
  19. Systemic IGF-1 gene delivery by rAAV9 improves spontaneous autoimmune peripheral polyneuropathy (SAPP)
    Authors: T Gao, N Bogdanova, S Ghauri, G Zhang, J Lin, K Sheikh
    Sci Rep, 2018-04-03;8(1):5408.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Immunization with Bacillus Calmette-Gu�rin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain
    Authors: Z Zuo, F Qi, J Yang, X Wang, Y Wu, Y Wen, Q Yuan, J Zou, K Guo, ZB Yao
    Neurobiol. Dis, 2017-02-09;101(0):27-39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Combined Effects of Androgen and Growth Hormone on Osteoblast Marker Expression in Mouse C2C12 and MC3T3-E1 Cells Induced by Bone Morphogenetic Protein
    Authors: K Kimura, T Terasaka, N Iwata, T Katsuyama, M Komatsubar, R Nagao, K Inagaki, F Otsuka
    J Clin Med, 2017-01-05;6(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism
    Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang
    Sci Rep, 2016-01-14;6(0):19404.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Survival of neural stem cell grafts in the lesioned spinal cord is enhanced by a combination of treadmill locomotor training via insulin-like growth factor-1 signaling.
    Authors: Hwang D, Shin H, Kwon M, Choi J, Ryu B, Kim B
    J Neurosci, 2014-09-17;34(38):12788-800.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  24. Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength.
    Authors: Brisson, Becky K, Spinazzola, Janelle, Park, SooHyun, Barton, Elisabet
    Am J Physiol Endocrinol Metab, 2014-02-25;306(8):E965-74.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  25. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
    Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
    J. Neurochem., 2009-09-24;111(6):1409-24.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Role of IGF signaling in olfactory sensory map formation and axon guidance.
    Authors: Scolnick JA, Cui K, Duggan CD, Xuan S, Yuan XB, Efstratiadis A, Ngai J
    Neuron, 2008-03-27;57(6):847-57.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  27. Low-dose GH supplementation reduces the TLR2 and TNF-alpha expressions in visceral fat.
    Authors: Kubota Y, Unoki H, Bujo H, Rikihisa N, Udagawa A, Yoshimoto S, Ichinose M, Saito Y
    Biochem. Biophys. Res. Commun., 2008-01-15;368(1):81-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  28. Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1.
    Authors: Butovsky O, Koronyo-Hamaoui M, Kunis G, Ophir E, Landa G, Cohen H, Schwartz M
    Proc. Natl. Acad. Sci. U.S.A., 2006-07-24;103(31):11784-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  29. Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis.
    Authors: Butovsky O, Landa G, Kunis G, Ziv Y, Avidan H, Greenberg N, Schwartz A, Smirnov I, Pollack A, Jung S, Schwartz M
    J. Clin. Invest., 2006-03-23;116(4):905-15.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  30. Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
    Authors: Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M
    Mol. Cell. Neurosci., 2005-11-16;31(1):149-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  31. Longevity effect of IGF-1R(+/-) mutation depends on genetic background-specific receptor activation
    Authors: Jie Xu, Géraldine Gontier, Zayna Chaker, Philippe Lacube, Joëlle Dupont, Martin Holzenberger
    Aging Cell
  32. Pro-Resolving Factors Released by Macrophages After Efferocytosis Promote Mucosal Wound Healing in Inflammatory Bowel Disease
    Authors: Omayra Martin-Rodriguez, Thierry Gauthier, Francis Bonnefoy, Mélanie Couturier, Anna Daoui, Cécile Chagué et al.
    Frontiers in Immunology
  33. Stimulation of neoplastic mouse lung cell proliferation by alveolar macrophage-derived, insulin-like growth factor-1 can be blocked by inhibiting MEK and PI3K activation
    Authors: Jason M Fritz, Lori D Dwyer-Nield, Alvin M Malkinson
    Molecular Cancer
  34. Induction of a regenerative microenvironment in skeletal muscle is sufficient to induce embryonal rhabdomyosarcoma in p53-deficient mice
    Authors: Marybeth Camboni, Sue Hammond, Laura T Martin, Paul T Martin
    The Journal of Pathology

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Mouse IGF-I/IGF-1 Antibody
By Rika Ohkubo on 04/18/2019
Application: WB Sample Tested: Purified protein Species: Mouse

Could not detect IGF-1 when I used liver samples from mice.

Western Blot Mouse IGF-I/IGF-1 Antibody AF791