Mouse IGFBP-2 Biotinylated Antibody

Catalog #: BAF797
4 Citations Datasheet / COA / SDS

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BAF797

All IGFBP-2 Antibodies

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IGFBP-2 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IGFBP-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) IGFBP‑2, rhIGFBP-1, rhIGFBP-3, rhIGFBP-4, rhIGFBP-5, rhIGFBP-6, rhIGF-I, rhIGF-II, recombinant mouse (rm) IGFBP‑3, rmIGFBP‑5, and rmIGFBP‑6 is observed. In direct ELISAs, no cross-reactivity with rmIGFBP-4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IGFBP‑2
Glu35-Gln305
Accession # CAA57270

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IGFBP‑2 (Catalog # 797-B2)

Mouse IGFBP-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse IGFBP‑2 Antibody (Catalog # MAB7971)

Standard: Recombinant Mouse IGFBP-2 Protein, CF (Catalog # 797-B2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-2

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Mouse IGFBP-2 cDNA encodes a 305 amino acid (aa) residue precursor protein with a putative 34 aa residue signal peptide that is processed to generate the 271 aa residue mature protein. Mouse and human IGFBP-2 share approximately 82% homology. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF-II, exhibiting a 2-10 fold higher affinity for IGF-II than for IGF-I.

References

Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
Landwehr, J. et al. (1993) Gene 124:281.

Long Name

Insulin-like Growth Factor Binding Protein 2

Entrez Gene IDs

3485 (Human); 16008 (Mouse)

Alternate Names

BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP2; IGFBP-2; IGF-BP53; insulin-like growth factor binding protein 2 (36kD); insulin-like growth factor binding protein 2, 36kDa; insulin-like growth factor-binding protein 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse IGFBP-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Spatial distribution of insulin-like growth factor binding protein-2 following hypoxic-ischemic injury.
Authors: Fletcher L, Isgor E, Sprague S, Williams L, Alajajian B, Jimenez D, Digicaylioglu M
BMC Neurosci, 2013-12-21;14(0):158.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Detection)
Endocrine actions of myostatin: systemic regulation of the IGF and IGF binding protein axis.
Authors: Williams NG, Interlichia JP, Jackson MF
Endocrinology, 2010-12-08;152(1):172-80.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development
Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation.
Authors: Yamada PM, Mehta HH, Hwang D
Endocrinology, 2010-10-06;151(12):5741-50.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development
Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model.
Authors: Raina K, Blouin MJ, Singh RP, Majeed N, Deep G, Varghese L, Glode LM, Greenberg NM, Hwang D, Cohen P, Pollak MN, Agarwal R
Cancer Res., 2007-11-15;67(22):11083-91.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development