Mouse IL-1 beta /IL-1F2 Antibody Summary
Accession # P10749
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Mouse IL‑1 beta /IL‑1F2 Antibody. Recombinant Mouse IL-1 beta /IL-1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB4012). The ND50 is typically 0.3-1.5 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Citations for Mouse IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
13
Citations: Showing 1 - 10
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PINK1 and PARK2 Suppress Pancreatic Tumorigenesis through Control of Mitochondrial Iron-Mediated Immunometabolism
Authors: Li C, Zhang Y, Cheng X et al.
Dev. Cell
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Caspase-1 induces smooth muscle cell growth in hypoxia-induced pulmonary hypertension
Authors: Camilla Udjus, Fadila T. Cero, Bente Halvorsen, Dina Behmen, Cathrine R. Carlson, Bård A. Bendiksen et al.
American Journal of Physiology-Lung Cellular and Molecular Physiology
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IL-1 beta drives production of FGF-23 at the onset of chronic kidney disease in mice
Authors: Mcknight Q, Jenkins S, Li X et Al.
J. Bone Miner. Res.
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Cornus officinalis with high pressure wine steaming enhanced anti-hepatic fibrosis: Possible through SIRT3-AMPK axis
Authors: Han, X;Ning, Y;Dou, X;Wang, Y;Shan, Q;Shi, K;Wang, Z;Ding, C;Hao, M;Wang, K;Peng, M;Kuang, H;Yang, Q;Sang, X;Cao, G;
Journal of pharmaceutical analysis
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death
Authors: T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek
Cell. Signal., 2020-06-15;73(0):109690.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Cells
Applications: Neutralization, Western Blot -
Mycobacterium tuberculosis Limits Host Glycolysis and IL-1&beta by Restriction of PFK-M via MicroRNA-21
Authors: EE Hackett, H Charles-Me, SM O'Leary, LE Gleeson, N Muñoz-Wolf, S Case, A Wedderburn, DGW Johnston, MA Williams, A Smyth, M Ouimet, KJ Moore, EC Lavelle, SC Corr, SV Gordon, J Keane, FJ Sheedy
Cell Rep, 2020-01-07;30(1):124-136.e4.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Nicotinamide phosphoribosyltransferase aggravates inflammation and promotes atherosclerosis in ApoE knockout mice
Authors: YY Kong, GQ Li, WJ Zhang, X Hua, CC Zhou, TY Xu, ZY Li, P Wang, CY Miao
Acta Pharmacol. Sin., 2019-03-04;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Cholinergic anti-inflammatory pathway inhibits neointimal hyperplasia by suppressing inflammation and oxidative stress
Authors: DJ Li, H Fu, J Tong, YH Li, LF Qu, P Wang, FM Shen
Redox Biol, 2017-11-16;15(0):22-33.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Key Role of the Scavenger Receptor MARCO in Mediating Adenovirus Infection and Subsequent Innate Responses of Macrophages
Authors: MD Maler, PJ Nielsen, N Stichling, I Cohen, Z Ruzsics, C Wood, P Engelhard, M Suomalaine, I Gyory, M Huber, J Müller-Que, WWA Schamel, S Gordon, T Jakob, SF Martin, W Jahnen-Dec, UF Greber, MA Freudenber, G Fejer
MBio, 2017-08-01;8(4):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Cigarette smoke-induced accumulation of lung dendritic cells is interleukin-1alpha-dependent in mice.
Authors: Botelho F, Nikota J, Bauer C, Morissette M, Iwakura Y, Kolbeck R, Finch D, Humbles A, Stampfli M
Respir Res, 2012-09-19;13(0):81.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
IL-1alpha/IL-1R1 expression in chronic obstructive pulmonary disease and mechanistic relevance to smoke-induced neutrophilia in mice.
Authors: Botelho F, Bauer C, Finch D, Nikota J, Zavitz C, Kelly A, Lambert K, Piper S, Foster M, Goldring J, Wedzicha J, Bassett J, Bramson J, Iwakura Y, Sleeman M, Kolbeck R, Coyle A, Humbles A, Stampfli M
PLoS ONE, 2011-12-06;6(12):e28457.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation.
Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
J. Immunol., 2011-09-19;187(9):4835-43.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Ex vivo culture of mouse skin activates an interleukin 1 alpha‐dependent inflammatory response
Authors: Hong‐Ming Zhou, Radomir M. Slominski, Leroy J. Seymour, Maria C. Bell, Priya Dave, Joseph Atumonye et al.
Experimental Dermatology
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This antibody was paired with BAF401 to create an ELISA to measure IL-1beta in mouse samples.
