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Mouse IL-3R beta Antibody

Catalog #: AF549
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF549
AF549-SP
Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 R beta Antibody.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-3R beta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑3 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Common  beta  chain is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-3 R beta
His23-Trp440
Accession # P26954
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑3 R beta Fc Chimera (Catalog # 549-R3)
Flow Cytometry
0.25 µg/106 cells
DA3 mouse myeloma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑3-induced proliferation in the NFS‑60 mouse myelogenous leukemia lymphoblast cell line. Holmes, K. L. et al. (1985) Proc. Natl. Acad. Sci. USA 82:6687. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 0.5 ng/mL Recombinant Mouse IL‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 R beta Antibody. View Larger

Cell Proliferation Induced by IL‑3 and Neutralization by Mouse IL‑3 R beta Antibody. Recombinant Mouse IL-3 (Catalog # 403-ML) stimulates proliferation in the NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse IL-3 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF549). The ND50 is typically 5-15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3R beta

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha  and the beta  subunits are members of the cytokine receptor superfamily (3).

References
  1. Itoh, N. et al. (1990) Science 247:324.
  2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
  3. Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.
Long Name
Interleukin 3 Receptor beta
Entrez Gene IDs
12984 (Mouse)
Alternate Names
AIC2A; BetaIl3; Bil3; Csfgmrb; IL3R beta; IL-3R beta; IL3R; IL3RB; IL-3Rb; Il3rb2

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Citations for Mouse IL-3R beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Modified Vaccinia Virus Ankara Preferentially Targets Antigen Presenting Cells In Vitro, Ex Vivo and In Vivo
    Authors: AF Altenburg, CE van de San, BWS Li, RJ MacLoughli, RAM Fouchier, G van Ameron, A Volz, RW Hendriks, RL de Swart, G Sutter, GF Rimmelzwaa, RD de Vries
    Sci Rep, 2017-08-17;7(1):8580.
  2. ABCA1 Exerts Tumor-Suppressor Function in Myeloproliferative Neoplasms
    Authors: M Viaud, O Abdel-Waha, J Gall, S Ivanov, R Guinamard, S Sore, J Merlin, M Ayrault, E Guilbaud, A Jacquel, P Auberger, N Wang, RL Levine, AR Tall, L Yvan-Charv
    Cell Rep, 2020-03-10;30(10):3397-3410.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity
    Authors: S Kimura, Y Nakamura, N Kobayashi, K Shiroguchi, E Kawakami, M Mutoh, H Takahashi-, T Yamada, M Hisamoto, M Nakamura, N Udagawa, S Sato, T Kaisho, T Iwanaga, K Hase
    Nat Commun, 2020-01-13;11(1):234.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC/IF

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