Mouse IL-3R beta Biotinylated Antibody

Catalog #: BAF549 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF549

All IL-3R beta Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse IL-3R beta Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑3 R beta in ELISAs and Western blots. In sandwich immunoassays, less than 0.005% cross‑reactivity with recombinant mouse (rm) IL-3, rmIL-3 R alpha, and recombinant human IL-3 R alpha is observed. In Western blots, less than 1% cross-reactivity with recombinant mouse IL‑3 R alpha is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-3 R beta
His23-Trp440
Accession # P26954

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑3 R beta Fc Chimera (Catalog # 549-R3)

Flow Cytometry

2.5 µg/10⁶ cells

DA3 mouse myeloma cell line

Mouse IL-3 R beta Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse IL-3R beta Antibody (Catalog # MAB5491)

Standard: Recombinant Mouse IL-3R beta Fc Chimera Protein, CF (Catalog # 549-R3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3R beta

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha and the beta subunits are members of the cytokine receptor superfamily (3).

References

Itoh, N. et al. (1990) Science 247:324.
Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.

Long Name

Interleukin 3 Receptor beta

Entrez Gene IDs

12984 (Mouse)

Alternate Names

AIC2A; BetaIl3; Bil3; Csfgmrb; IL3R beta; IL-3R beta; IL3R; IL3RB; IL-3Rb; Il3rb2