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Mouse Leptin/OB Antibody

Catalog #: MAB498
1 Citation Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB498-100
MAB498-SP
Detection of Mouse Leptin/OB by Western Blot.
3 Images
Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Leptin/OB Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Leptin/OB in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2299B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse Leptin/OB
Val22-Cys167
Accession # Q544U0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
ELISA

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (Catalog # MAB4981).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Leptin DuoSet ELISA Kit (Catalog # DY498-05) for convenient development of a sandwich ELISA or the Mouse/Rat Leptin Quantikine ELISA Kit (Catalog # MOB00B) for  a complete optimized ELISA.

 
Immunohistochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Leptin/OB antibody by Western Blot. View Larger

Detection of Mouse Leptin/OB by Western Blot. Western blot shows lysates of mouse ovary tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (Catalog # MAB498) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Leptin/OB at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Leptin/OB antibody in Human Adipose Tissue by Immunohistochemistry (IHC-P). View Larger

Leptin/OB in Human Adipose Tissue. Leptin/OB was detected in immersion fixed paraffin-embedded sections of human adipose tissue using Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (Catalog # MAB498) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in fat cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

ELISA View Larger

Mouse Leptin/OB ELISA Standard Curve. Recombinant Mouse Leptin/OB protein was serially diluted 2-fold and captured by Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (Catalog # MAB498) coated on a Clear Polystyrene Microplate (DY990). Rabbit Anti-Mouse Leptin/OB Monoclonal Antibody (MAB4981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. Mouse Leptin shares approximately 96% and 84% sequence identity with the rat and human protein, respectively. Mouse Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1-3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.

References
  1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
  2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Entrez Gene IDs
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

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Citation for Mouse Leptin/OB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Leptin Promotes Angiogenesis via Pericyte STAT3 Pathway upon Intracerebral Hemorrhage
    Authors: Q Cui, Y Zhang, N Tian, J Yang, D Ya, W Xiang, Z Zhou, Y Jiang, J Deng, B Yang, X Lin, Q Li, R Liao
    Cells, 2022-09-03;11(17):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

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