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Mouse M-CSF Quantikine ELISA Kit

Catalog #: MMC00
32 Citations 3 Reviews Datasheet / COA / SDS
Newer Version Available: MMC00B
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MMC00 has been discontinued and is replaced by MMC00B.

Mouse M-CSF ELISA Standard Curve
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Product Details
Procedure
Citations (32)
FAQs
Reviews (3)
Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse M-CSF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (13 uL), Serum (13 uL), EDTA Plasma (13 uL)
Sensitivity
5 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse M-CSF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse M-CSF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse M-CSF levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse M-CSF and antibodies raised against the recombinant factor. Results obtained for naturally occurring mouse M-CSF showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse M-CSF.

Recovery

The recovery of mouse M-CSF spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 104 85-120
EDTA Plasma (n=6) 101 89-112
Serum (n=6) 97 85-115

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse M-CSF in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse M-CSF ELISA Linearity

Scientific Data

N/A Mouse M-CSF ELISA Standard Curve View Larger

Mouse M-CSF ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Long Name:
Macrophage Colony Stimulating Factor
Entrez Gene IDs:
1435 (Human); 12977 (Mouse)
Alternate Names:
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse M-CSF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
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  1. Transitional premonocytes emerge in the periphery for host defense against bacterial infections
    Authors: YC Teh, MY Chooi, D Liu, I Kwok, GC Lai, L Ayub Ow Yo, M Ng, JLY Li, Y Tan, M Evrard, L Tan, KH Liong, K Leong, CC Goh, AYJ Chan, NB Shadan, CK Mantri, YY Hwang, H Cheng, T Cheng, W Yu, HL Tey, A Larbi, A St John, V Angeli, C Ruedl, B Lee, F Ginhoux, SL Chen, LG Ng, JL Ding, SZ Chong
    Science Advances, 2022-03-04;8(9):eabj4641.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  2. M-CSF supports medullary erythropoiesis and erythroid iron demand following burn injury through its activity on homeostatic iron recycling
    Authors: JG Noel, SW Ramser, L Pitstick, JP Bonamer, B Mackenzie, KG Seu, TA Kalfa, JA Cancelas, JC Gardner
    Scientific Reports, 2022-01-24;12(1):1235.
    Species: Mouse
    Sample Types: Serum
  3. Elevated expression of the colony-stimulating factor 1 (CSF1) induces prostatic intraepithelial neoplasia dependent of epithelial-Gp130
    Authors: OJ Kwon, B Zhang, D Jia, L Zhang, X Wei, Z Zhou, D Liu, KT Huynh, K Zhang, Y Zhang, P Labhart, A Sboner, C Barbieri, MC Haffner, CJ Creighton, L Xin
    Oncogene, 2022-01-08;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Biomimetic nanoparticles deliver mRNAs encoding costimulatory receptors and enhance T cell mediated cancer immunotherapy
    Authors: W Li, X Zhang, C Zhang, J Yan, X Hou, S Du, C Zeng, W Zhao, B Deng, DW McComb, Y Zhang, DD Kang, J Li, WE Carson, Y Dong
    Nature Communications, 2021-12-14;12(1):7264.
    Species: Mouse
    Sample Types: Plasma
  5. Hepatocyte-specific glucose-6-phosphatase deficiency disturbs platelet aggregation and decreases blood monocytes upon fasting-induced hypoglycemia
    Authors: AM La Rose, V Bazioti, JA Hoogerland, AF Svendsen, AG Groenen, M van Faasse, MGS Rutten, NJ Kloosterhu, B Dethmers-A, JH Nijland, G Mithieux, F Rajas, F Kuipers, MV Lukens, O Soehnlein, MH Oosterveer, M Westerterp
    Molecular Metabolism, 2021-06-04;53(0):101265.
    Species: Mouse
    Sample Types: Plasma
  6. Pharmacological normalization of pancreatic cancer-associated fibroblast secretome impairs pro-metastatic cross-talk with macrophages: Stromal CSF-1 facilitates metastasis
    Authors: R Samain, A Brunel, T Douché, M Fanjul, S Cassant-So, J Rochotte, J Cros, C Neuzillet, J Raffenne, C Duluc, A Perraud, J Nigri, V Gigoux, I Bieche, M Ponzo, G Carpentier, I Cascone, R Tomasini, HA Schmid, M Mathonnet, R Nicolle, MP Bousquet, Y Martineau, S Pyronnet, C Jean, C Bousquet
    Cellular and Molecular Gastroenterology and Hepatology, 2021-01-20;0(0):.
    Species: Mouse
    Sample Types: Plasma
  7. Tumor derived UBR5 promotes ovarian cancer growth and metastasis through inducing immunosuppressive macrophages
    Authors: M Song, OO Yeku, S Rafiq, T Purdon, X Dong, L Zhu, T Zhang, H Wang, Z Yu, J Mai, H Shen, B Nixon, M Li, RJ Brentjens, X Ma
    Nature Communications, 2020-12-08;11(1):6298.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. CSF1R signaling is a regulator of pathogenesis in progressive MS
    Authors: N Hagan, JL Kane, D Grover, L Woodworth, C Madore, J Saleh, J Sancho, J Liu, Y Li, J Proto, M Zelic, A Mahan, M Kothe, AA Scholte, M Fitzgerald, B Gisevius, A Haghikia, O Butovsky, D Ofengeim
    Cell Death Dis, 2020-10-23;11(10):904.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. Effective hematopoietic stem cell-based gene therapy in a murine model of hereditary pulmonary alveolar proteinosis
    Authors: M Hetzel, E Lopez-Rodr, A Mucci, AHH Nguyen, T Suzuki, K Shima, T Buchegger, S Dettmer, T Rodt, JP Bankstahl, P Malik, L Knudsen, A Schambach, G Hansen, BC Trapnell, N Lachmann, T Moritz
    Haematologica, 2019-07-09;105(4):1147-1157.
    Species: Mouse
    Sample Types: Bronchoalveolar lavage fluid
  10. Mesothelial cell CSF1 sustains peritoneal macrophage proliferation
    Authors: S Ivanov, A Gallerand, M Gros, MI Stunault, J Merlin, N Vaillant, L Yvan-Charv, RR Guinamard
    Eur. J. Immunol., 2019-07-05;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. A Selective FGFR inhibitor AZD4547 suppresses RANKL/M-CSF/OPG-dependent ostoclastogenesis and breast cancer growth in the metastatic bone microenvironment
    Authors: J Kang, YJ Choi, BY Seo, U Jo, SI Park, YH Kim, KH Park
    Sci Rep, 2019-06-19;9(1):8726.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Macrophage-derived granulin drives resistance to immune checkpoint inhibition in metastatic pancreatic cancer
    Authors: V Quaranta, C Rainer, SR Nielsen, ML Raymant, MS Ahmed, DD Engle, A Taylor, T Murray, F Campbell, DH Palmer, DA Tuveson, A Mielgo, MC Schmid
    Cancer Res., 2018-05-22;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. A Macrophage Colony-Stimulating Factor-Producing ?? T Cell Subset Prevents Malarial Parasitemic Recurrence
    Authors: MR Mamedov, A Scholzen, RV Nair, K Cumnock, JA Kenkel, JHM Oliveira, DL Trujillo, N Saligrama, Y Zhang, F Rubelt, DS Schneider, YH Chien, RW Sauerwein, MM Davis
    Immunity, 2018-02-06;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  14. Circuit Design Features of a Stable Two-Cell System
    Authors: X Zhou, RA Franklin, M Adler, JB Jacox, W Bailis, JA Shyer, RA Flavell, A Mayo, U Alon, R Medzhitov
    Cell, 2018-02-01;172(4):744-757.e17.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Calf Spleen Extractive Injection protects mice against cyclophosphamide-induced hematopoietic injury through G-CSF-mediated JAK2/STAT3 signaling
    Authors: W Lu, D Jia, S An, M Mu, X Qiao, Y Liu, X Li, D Wang
    Sci Rep, 2017-08-21;7(1):8402.
    Species: Mouse
    Sample Types: Plasma
  16. iRhom2 regulates CSF1R cell surface expression and non-steady state myelopoiesis in mice
    Eur J Immunol, 2016-09-28;0(0):.
    Species: Mouse
    Sample Types: Serum
  17. Monocyte/macrophage lineage commitment and distribution are affected by the lack of regulatory T cells in scurfy mice
    Eur J Immunol, 2016-05-27;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. SHIP1-expressing mesenchymal stem cells regulate hematopoietic stem cell homeostasis and lineage commitment during aging.
    Authors: Iyer S, Brooks R, Gumbleton M, Kerr W
    Stem Cells Dev, 2015-02-05;24(9):1073-81.
    Species: Mouse
    Sample Types: Serum
  19. Blockade of Fas signaling in breast cancer cells suppresses tumor growth and metastasis via disruption of Fas signaling-initiated cancer-related inflammation.
    Authors: Liu Q, Tan Q, Zheng Y, Chen K, Qian C, Li N, Wang Q, Cao X
    J Biol Chem, 2014-03-13;289(16):11522-35.
    Species: Mouse
    Sample Types: Whole Cells
  20. KIT oncogene inhibition drives intratumoral macrophage M2 polarization.
    Authors: Cavnar, Michael, Zeng, Shan, Kim, Teresa S, Sorenson, Eric C, Ocuin, Lee M, Balachandran, Vinod P, Seifert, Adrian M, Greer, Jonathan, Popow, Rachel, Crawley, Megan H, Cohen, Noah A, Green, Benjamin, Rossi, Ferdinan, Besmer, Peter, Antonescu, Cristina, DeMatteo, Ronald P
    J Exp Med, 2013-12-09;210(13):2873-86.
    Species: Mouse
    Sample Types: Serum
  21. Steady-state neutrophil homeostasis is dependent on TLR4/TRIF signaling.
    Authors: Bugl S, Wirths S, Radsak M, Schild H, Stein P, Andre M, Muller M, Malenke E, Wiesner T, Marklin M, Frick J, Handgretinger R, Rammensee H, Kanz L, Kopp H
    Blood, 2012-12-05;121(5):723-33.
    Species: Mouse
    Sample Types: Plasma
  22. The combination of the histone deacetylase inhibitor vorinostat and synthetic triterpenoids reduces tumorigenesis in mouse models of cancer.
    Authors: Tran, Kim, Risingsong, Renee, Royce, Darlene, Williams, Charlott, Sporn, Michael, Pioli, Patricia, Gediya, Lalji K, Njar, Vincent, Liby, Karen T
    Carcinogenesis, 2012-10-06;34(1):199-210.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization.
    Authors: Miyamoto K, Yoshida S, Kawasumi M
    J. Exp. Med., 2011-10-17;208(11):2175-81.
    Species: Mouse
    Sample Types: Serum
  24. Temporal changes in myeloid cells in the cervix during pregnancy and parturition.
    Authors: Timmons BC, Fairhurst AM, Mahendroo MS
    J. Immunol., 2009-03-01;182(5):2700-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
    Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
    Arthritis Rheum., 2008-11-01;58(11):3510-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells.
    Authors: Islam S, Hassan F, Tumurkhuu G, Dagvadorj J, Koide N, Naiki Y, Mori I, Yoshida T, Yokochi T
    Biochem. Biophys. Res. Commun., 2007-06-12;360(2):346-51.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. IL-7 induces myelopoiesis and erythropoiesis.
    Authors: Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK
    J. Immunol., 2007-02-01;178(3):1553-63.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. Concerted action of Smad and CREB-binding protein regulates bone morphogenetic protein-2-stimulated osteoblastic colony-stimulating factor-1 expression.
    Authors: Ghosh-Choudhury N, Singha PK, Woodruff K, St Clair P, Bsoul S, Werner SL, Choudhury GG
    J. Biol. Chem., 2006-05-17;281(29):20160-70.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. Perforin-deficient CD8+ T cells mediate fatal lymphocytic choriomeningitis despite impaired cytokine production.
    Authors: Storm P, Bartholdy C, Sorensen MR, Christensen JP, Thomsen AR
    J. Virol., 2006-02-01;80(3):1222-30.
    Species: Mouse
    Sample Types: Serum
  30. The cell-surface isoform of colony stimulating factor 1 (CSF1) restores but does not completely normalize fecundity in CSF1-deficient mice.
    Authors: Ovadia S, Insogna K, Yao GQ
    Biol. Reprod., 2005-10-19;74(2):331-6.
    Species: Mouse
    Sample Types: Tissue Homogenates
  31. Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation.
    Authors: Sergejeva S, Ivanov S, Lotvall J, Linden A
    Am. J. Respir. Cell Mol. Biol., 2005-05-18;33(3):248-53.
    Species: Mouse
    Sample Types: BALF
  32. Differential roles of CC chemokine ligand 2/monocyte chemotactic protein-1 and CCR2 in the development of T1 immunity.
    Authors: Traynor TR, Herring AC, Dorf ME, Kuziel WA, Toews GB, Huffnagle GB
    J. Immunol., 2002-05-01;168(9):4659-66.
    Species: Mouse
    Sample Types: BALF

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