Mouse Metadherin Antibody Summary
Lys167-Ser297
Accession # Q80WJ7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Metadherin by Western Blot. Western blot shows lysates of 4T1 mouse breast cancer cell line and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Metadherin Monoclonal Antibody (Catalog # MAB7180) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Metadherin at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Metadherin
Metadherin (MTDH; also Lyric and Astrocyte-elevated gene-1/AEG1) is a unique molecule originally discovered to be upregulated in astrocytes following HIV infection. Although its predicted MW is 64 kDa, it runs anomalously at approximately 80 kDa in SDS-Page. It is widely expressed, and appears to be a component of the ER, nucleolus and inner nuclear membrane. Metadherin is reported to promote Akt/PI3 kinase activity, and suppress apoptosis-associated FOXO3A transcription. Mouse Metadherin is a type III (i.e.- a type I with no signal sequence) transmembrane protein 579 amino acids (aa) in length. It contains a luminal N-terminus (aa 1-48) with a 510 aa cytoplasmic C-terminus. There are no identifiable structural motifs, although two poly-Lys segments and two utilized Ser phosphorylation sites exist. Multiple bands at 80 kDa, 75 kDa, 50-55 kDa and 37 kDa are seen for Metadherin on SDS-Page. They may reflect the presence of at least two potential isoform variants that show 1) a deletion of aa 422-457, and 2) an Ile substitution for aa 190-579. Over aa 167-297, mouse Metadherin shares 98% and 97% aa sequence identity with rat and human Metadherin, respectively.
Product Datasheets
Citation for Mouse Metadherin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Ezrin Binds to DEAD-Box RNA Helicase DDX3 and Regulates Its Function and Protein Level
Authors: Haydar Çelik, Kamal P. Sajwan, Saravana P. Selvanathan, Benjamin J. Marsh, Amrita V. Pai, Yasemin Saygideger Kont et al.
Molecular and Cellular Biology
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