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Mouse Meteorin-like/METRNL DuoSet ELISA

Catalog #: DY6679
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY6679
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse Meteorin-like / METRNL ELISA Standard Curve
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (1)
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse Meteorin-like/METRNL DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
187.5 - 6,000 pg/mL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Meteorin-like / METRNL. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Mouse Meteorin-like / METRNL ELISA Standard Curve View Larger

Mouse Meteorin-like / METRNL ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Meteorin-like/METRNL

METRNL (Meteorin-like) is a cytoplasmic 30 kDa (predicted) member of the meteorin family of proteins. The name meteorin derives from the ability of the founding family member meteorin to transform glia into cells containing an extensive, meteor-like tail. It is expressed by neurons, and appears to function as a primer molecule that sets the stage for either cell differentiation or neurite outgrowth when it acts with Dclk1 and SerpinB1. Mature human METRNL is 266 amino acids (aa) in length (aa 46-311). It reportedly contains a signal sequence (aa 1-45), which suggests it may be secreted. Mature human and mouse METRNL share 79% aa sequence identity.

Entrez Gene IDs:
284207 (Human); 210029 (Mouse); 316842 (Rat)
Alternate Names:
Meteorin like; meteorin, glial cell differentiation regulator-like; meteorin-like protein; Meteorin-like; METRNL; MGC99788

Citations for Mouse Meteorin-like/METRNL DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Meteorin-like is an injectable peptide that can enhance regeneration in aged muscle through immune-driven fibro/adipogenic progenitor signaling
    Authors: DE Lee, LK McKay, A Bareja, Y Li, A Khodabukus, N Bursac, GA Taylor, GS Baht, JP White
    Nature Communications, 2022-12-09;13(1):7613.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Meteorin links the bone marrow hypoxic state to hematopoietic stem/progenitor cell mobilization
    Authors: YW Dai, JK Ma, R Jiang, XL Zhan, SY Chen, LL Feng, Q Zhang, TB Liang, K Lv, GJ Yang, JF Lu, J Chen, XJ Lu
    Cell Reports, 2022-09-20;40(12):111361.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Exercise Inhibits NLRP3 Inflammasome Activation in Obese Mice via the Anti-Inflammatory Effect of Meteorin-like
    Authors: HMA Javaid, NE Sahar, DL ZhuGe, JY Huh
    Cells, 2021-12-09;10(12):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Eosinophil function in adipose tissue is regulated by Kr�ppel-like factor 3 (KLF3)
    Authors: AJ Knights, EJ Vohralik, PJ Houweling, ES Stout, LJ Norton, SJ Alexopoulo, JJ Yik, H Mat Jusoh, EM Olzomer, KS Bell-Ander, KN North, KL Hoehn, M Crossley, KGR Quinlan
    Nat Commun, 2020-06-10;11(1):2922.
    Species: Mouse
    Sample Types: Plasma
  5. METRNL attenuates lipid-induced inflammation and insulin resistance via AMPK or PPAR?-dependent pathways in skeletal muscle of mice
    Authors: TW Jung, SH Lee, HC Kim, JS Bang, AM Abd El-Aty, A Hac?müftüo, YK Shin, JH Jeong
    Exp. Mol. Med., 2018-09-13;50(9):122.
    Species: Mouse
    Sample Types: Serum
  6. Aerobic Exercise Increases Meteorin-Like Protein in Muscle and Adipose Tissue of Chronic High-Fat Diet-Induced Obese Mice
    Authors: JY Bae
    Biomed Res Int, 2018-04-30;2018(0):6283932.
    Species: Mouse
    Sample Types: Plasma
  7. Effects of detraining and retraining on muscle energy-sensing network and meteorin-like levels in obese mice
    Authors: JY Bae, J Woo, S Kang, KO Shin
    Lipids Health Dis, 2018-04-27;17(1):97.
    Species: Mouse
    Sample Types: Plasma
  8. Adipocyte Metrnl Antagonizes Insulin Resistance Through PPARgamma Signaling.
    Authors: Li Z, Song J, Zheng S, Fan M, Guan Y, Qu Y, Xu J, Wang P, Miao C
    Diabetes, 2015-08-25;64(12):4011-22.
    Species: Mouse
    Sample Types: Serum
  9. METEORIN-LIKE is a cytokine associated with barrier tissues and alternatively activated macrophages.
    Authors: Ushach I, Burkhardt A, Martinez C, Hevezi P, Gerber P, Buhren B, Schrumpf H, Valle-Rios R, Vazquez M, Homey B, Zlotnik A
    Clin Immunol, 2014-12-05;156(2):119-127.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Reviews for Mouse Meteorin-like/METRNL DuoSet ELISA

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Mouse Meteorin-like/METRNL DuoSet ELISA
By Anonymous on 11/08/2018
Sample Tested: Serum
Mouse Meteorin-like/METRNL DuoSet ELISA DY6679