Mouse MINA Antibody

Catalog #: AF7266 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7266
AF7266-SP

Detection of Mouse MINA by Western Blot.

1 Image

All MINA Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MINA Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MINA in direct ELISAs and Western blots. In direct ELISAs, 80% cross-reactivity with recombinant human (rh) MINA is observed and 40% cross-reactivity with rhMINA is observed in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse MINA
Pro2-Gly192
Accession # Q8CD15

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Mouse MINA by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line and beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse MINA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7266) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for MINA at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MINA

MINA (myc-induced nuclear antigen; also Mina53) is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, occasional squamous epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R and IL-4. It may exert its regulatory activity through an intrinsic demethylase function. Mouse MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme-associated) region (aa 51-363) that contains a JmjC domain (aa 139-271). There are two potential isoform variants that contain either a 12 aa substitution for aa 145-465, or a 15 aa substitution for aa 228-465. Over aa 2-192, mouse MINA shares 92% and 82% aa sequence identity with rat and human MINA, respectively.

Long Name

Myc-induced Nuclear Antigen

Entrez Gene IDs

84864 (Human); 67014 (Mouse); 266670 (Rat)

Alternate Names

DKFZp762O1912; FLJ14393; MDIG; MDIGDKFZp762O1912; MINA; Mina53; MINA53mdig; mineral dust induced gene protein; Mineral dust-induced gene protein; MYC induced nuclear antigen; MYC-induced nuclear antigen; myc-induced nuclear antigen, 53 kDa; NO52; Nucleolar protein 52