Mouse PDGFRL Antibody

Catalog #: MAB9739 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9739-100
MAB9739-SP

Cell Proliferation Induced by PDGFRL and Neutralization by Mouse PDGFRL Antibody.

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All PDGFRL Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PDGFRL Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse PDGFRL in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 1010639

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived mouse PDGFRL
Gln22-Ser375
Accession # Q6PE55

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize PDGFRL-induced proliferation in the HCT-116 human colorectal carcinoma cell line. The Neutralization Dose (ND50) is typically 4-24 µg/mL in the presence of 10 μg/mL Recombinant Mouse PDGFRL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by PDGFRL and Neutralization by Mouse PDGFRL Antibody. Recombinant Mouse PDGFRL (Catalog # 9739-PR) stimulates proliferation in the HCT-116 human colorectal carcinoma cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse PDGFRL (10 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse PDGFRL Monoclonal Antibody (Catalog # MAB9739). The ND₅₀ is typically 4-24 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGFRL

Platelet-derived growth factor receptor-like protein (PDGFRL) is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, mouse PDGFRL is 90% similar to the human version and 95% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). The high frequency of loss of heterozygosity (LOH) near the gene of PDGFRL indicated in several studies that PDGFRL can be a tumor suppressor gene (TSG) in breast cancer (6-9), colorectal cancer, prostate cancer (10), non-small cell lung cancers (11) and hepatocellular carcinoma (12). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (13). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (14). Another study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation (15).

References

Guo, F-J. et al. (2010). World J Gastroenterol. 16:1465.
Andrae, J. et al. (2008) Genes Dev. 22:1276.
Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
Matsui, T. et al. (1989) Science 243:800.
Seitz, S. et al. (2006). Genes Chromosomes Cancer 45:612.
Yaremko, M.L. et al. (1996). Genes, Chromosome Cancer 16:189.
Seitz, S. et al. (2000). Eur J Cancer. 36:1507.
Rennstam, K. et al. (2003). Cancer Res. 63:8861.
Komiya, A. et al. (1997). Jpn J Cancer Res. 88:389.
Lerebours, F. et al. (1999). Int J Cancer. 81:854.
Kahng, Y.S. et al. (2003) J Gastroenterol Hepatol. 18:430.
Hou, S. et al. (2013). Hum Mutat. 34:74.
Fujikado N. et al. (2006). Arthritis Res.& Ther. 8:R100.
Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.

Long Name

Platelet-derived Growth Factor Receptor-like

Entrez Gene IDs

5157 (Human); 68797 (Mouse); 290771 (Rat)

Alternate Names

PDGFRL; PDGFR-Like Protein; PDGRL; platelet-derived growth factor receptor-like protein; platelet-derived growth factor receptor-like; platelet-derived growth factor-beta-like tumor suppressor; PRLTS; PRLTSPDGF receptor beta-like tumor suppressor