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Mouse/Rat CCL5/RANTES Quantikine ELISA Kit

Catalog #: MMR00
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Mouse/Rat CCL5/RANTES ELISA Standard Curve
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse/Rat CCL5/RANTES Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), Platelet-poor EDTA Plasma (25 uL), Platelet-poor Citrate Plasma (25 uL)
Sensitivity
2 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant mouse and rat RANTES.  This assay cross-reacts 1.3% with recombinant Human RANTES.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat CCL5/RANTES Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat RANTES levels in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant mouse RANTES as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant RANTES. Results obtained using natural mouse or rat RANTES showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse and rat RANTES.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Citrate Plasma, Platelet-poor Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 18.8 104 198 20.1 109 208
Standard Deviation 0.7 1.9 4.1 1.6 5.6 11.5
CV% 3.7 1.8 2.1 8 5.1 5.5

Recovery

The recovery of RANTES spiked to three levels in mouse samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=8) 108 98-115
Platelet-poor Citrate Plasma (n=5) 102 95-110
Platelet-poor EDTA Plasma (n=5) 99 86-110
Serum (n=5) 97 90-107

Linearity

To assess the linearity of the assay, four or more samples containing or spiked with various concentrations of RANTES in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse/Rat CCL5/RANTES ELISA Linearity
Mouse/Rat CCL5/RANTES ELISA Linearity

Scientific Data

N/A Mouse/Rat CCL5/RANTES ELISA Standard Curve View Larger

Mouse/Rat CCL5/RANTES ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell-expressed, and presumably Secreted), also known as CCL5, a member of the CC chemokine family of inflammatory and immunoregulatory cytokines, initially discovered by subtractive hybridization as a T cell-specific molecule (1, 2). Mouse RANTES cDNA encodes a 91 amino acid (aa) residue precursor protein with a presumed signal peptide of 23 aa residues that is cleaved to generate the 68 aa residue mature protein (3). At the amino acid sequence level, mouse RANTES is 84% identical to human RANTES. Both human and mouse RANTES exhibit cross species activity. Naturally occurring human RANTES has been found to be a mixture of the 68 aa residue mature protein and a 66 aa residue amino-terminally truncated form (4, 5). Cells known to express RANTES include keratinocytes, eosinophils, platelets, endothelium, fibroblasts, respiratory epithelium, astrocytes, vascular smooth muscle, and CD4+ and CD8+ T cells (6-14). As suggested by its acronym, RANTES production by the various cell types is induced in response to cytokine stimulation (8-13). 

Like other CC chemokines, RANTES is a monocyte chemoattractant (2). RANTES can also chemoattract unstimulated CD4+/CD45RO+ memory T cells and stimulated CD4+ and CD8+ T cells with the naive and memory phenotypes. In addition, RANTES can chemoattract and degranulate eosinophils, as well as chemoattract and induce histamine release from basophils. Human RANTES has been shown to be an inhibitor of HIV infection of human mononuclear cells (15, 16). Several CC chemokine receptors, including CCR-1, CCR-3, CCR-4, and CCR-5, have been shown to bind RANTES and subsequently to transduce a signal by increasing the intracellular calcium ion level (2, 17-21).

Entrez Gene IDs:
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names:
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat CCL5/RANTES Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

95 Citations: Showing 1 - 10
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  1. Establishment of a Rat Model of Alcoholic Liver Fibrosis with Simulated Human Drinking Patterns and Low-Dose Chemical Stimulation
    Authors: Lin, CY;Omoscharka, E;Liu, Y;Cheng, K;
    Biomolecules
    Species: Rat
    Sample Types: Tissue Homogenates
  2. LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis
    Authors: Sha, L;Li, J;Shen, H;Wang, Q;Meng, P;Zhang, X;Deng, Y;Zhu, W;Xu, Q;
    Cell reports
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Tetraspanin CD9-derived peptides inhibit Pseudomonas aeruginosa corneal infection and aid in wound healing of corneal epithelial cells
    Authors: Jadi, PK;Dave, A;Issa, R;Tabbasum, K;Okurowska, K;Samarth, A;Urwin, L;Green, LR;Partridge, LJ;MacNeil, S;Garg, P;Monk, PN;Roy, S;
    The ocular surface
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Novel neuroendocrine role of gamma-aminobutyric acid and gastrin releasing peptide in the host response to influenza infection
    Authors: K Ann Shirey, W Lai, M Sunday, F Cuttitta, J Cg Blanco, SN Vogel
    Mucosal Immunology, 2023-03-24;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Association between an Increased Serum CCL5 Level and Pathophysiology of Degenerative Joint Disease in the Temporomandibular Joint in Females
    Authors: H Watanabe, T Iori, JW Lee, TS Kajii, A Takakura, R Takao-Kawa, Y Kitagawa, Y Maruoka, T Iimura
    International Journal of Molecular Sciences, 2023-02-01;24(3):.
    Species: Rat
    Sample Types: Serum
  6. Asthma reduces glioma formation by T cell decorin-mediated inhibition of microglia
    Authors: J Chatterjee, S Sanapala, O Cobb, A Bewley, AK Goldstein, E Cordell, X Ge, JR Garbow, MJ Holtzman, DH Gutmann
    Nature Communications, 2021-12-08;12(1):7122.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Dysregulated oxalate metabolism is a driver and therapeutic target in atherosclerosis
    Authors: Y Liu, Y Zhao, Y Shukha, H Lu, L Wang, Z Liu, C Liu, Y Zhao, H Wang, G Zhao, W Liang, Y Fan, L Chang, A Yurdagul, CB Pattillo, AW Orr, M Aviram, B Wen, MT Garcia-Bar, J Zhang, W Liu, D Sun, T Hayek, YE Chen, O Rom
    Cell Reports, 2021-07-27;36(4):109420.
    Species: Mouse
    Sample Types: Plasma
  8. Fucoxanthin Ameliorates Oxidative Stress and Airway Inflammation in Tracheal Epithelial Cells and Asthmatic Mice
    Authors: SJ Wu, CJ Liou, YL Chen, SC Cheng, WC Huang
    Cells, 2021-05-25;10(6):.
    Species: Mouse
    Sample Types: BALF
  9. A New Mouse Model of Chronic Myocarditis Induced by Recombinant Bacille Calmette-Gu�rin Expressing a T-Cell Epitope of Cardiac Myosin Heavy Chain-&alpha
    Authors: K Tajiri, K Imanaka-Yo, Y Tsujimura, K Matsuo, M Hiroe, K Aonuma, M Ieda, Y Yasutomi
    International Journal of Molecular Sciences, 2021-01-14;22(2):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. CSF1R signaling is a regulator of pathogenesis in progressive MS
    Authors: N Hagan, JL Kane, D Grover, L Woodworth, C Madore, J Saleh, J Sancho, J Liu, Y Li, J Proto, M Zelic, A Mahan, M Kothe, AA Scholte, M Fitzgerald, B Gisevius, A Haghikia, O Butovsky, D Ofengeim
    Cell Death Dis, 2020-10-23;11(10):904.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Accelerated onset of CNS prion disease in mice co-infected with a gastrointestinal helminth pathogen during the preclinical phase
    Authors: DS Donaldson, BM Bradford, KJ Else, NA Mabbott
    Sci Rep, 2020-03-12;10(1):4554.
    Species: Mouse
    Sample Types: Serum
  12. Hippocampal CCR5/RANTES Elevations in a Rodent Model of Post-Traumatic Stress Disorder: Maraviroc (a CCR5 Antagonist) Increases Corticosterone Levels and Enhances Fear Memory Consolidation
    Authors: JJ Merino, V Muñetón-Go, C Muñetón-Gó, MÁ Pérez-Izqu, M Loscertale, A Toledano G
    Biomolecules, 2020-02-01;10(2):.
    Species: Rat
    Sample Types: Tissue Lysates
  13. Tyrphostin AG490 reduces inflammation and fibrosis in neonatal obstructive nephropathy
    Authors: M Gasparitsc, A Schieber, T Schaubeck, U Keller, M Cattaruzza, B Lange-Sper
    PLoS ONE, 2019-12-17;14(12):e0226675.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Effect of inactivated Streptococcus pneumoniae as non-pathogenic particles on the severity of pneumonia caused by respiratory syncytial virus infection in mice
    Authors: A Miyauchi, W Watanabe, T Akashi, S Hashiguchi, H Yoshida, C Sugita, M Kurokawa
    Toxicol Rep, 2019-05-31;6(0):514-520.
    Species: Mouse
    Sample Types: Bronchoalveolar lavage fluid
  15. CCR5 Signaling Promotes Murine and Human Hematopoietic Regeneration following Ionizing Radiation
    Authors: SO Piryani, AYF Kam, UT Vu, NJ Chao, PL Doan
    Stem Cell Reports, 2019-05-30;13(1):76-90.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Melatonin Inhibits Lipopolysaccharide-Induced Inflammation and Oxidative Stress in Cultured Mouse Mammary Tissue
    Authors: GM Yu, W Tan
    Mediators Inflamm., 2019-02-12;2019(0):8597159.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. JNK signalling mediates aspects of maternal immune activation: importance of maternal genotype in relation to schizophrenia risk
    Authors: RL Openshaw, J Kwon, A McColl, JM Penninger, J Cavanagh, JA Pratt, BJ Morris
    J Neuroinflammation, 2019-01-28;16(1):18.
    Species: Mouse
    Sample Types: Tissue Homogenates
  18. Bradykinin 1 receptor blockade subdues systemic autoimmunity, renal inflammation, and blood pressure in murine lupus nephritis
    Authors: L Qin, Y Du, H Ding, A Haque, J Hicks, C Pedroza, C Mohan
    Arthritis Res. Ther., 2019-01-08;21(1):12.
    Species: Mouse
    Sample Types: Urine
  19. Toll-like receptor 3 activation impairs excitability and synaptic activity via TRIF signalling in immature rat and human neurons
    Authors: L Ritchie, R Tate, LH Chamberlai, G Robertson, M Zagnoni, T Sposito, S Wray, JA Wright, CE Bryant, NJ Gay, TJ Bushell
    Neuropharmacology, 2018-03-02;135(0):1-10.
    Species: Rat
    Sample Types: Cell Culture Supernates
  20. Sox7 promotes high-grade glioma by increasing VEGFR2-mediated vascular abnormality
    Authors: IK Kim, K Kim, E Lee, DS Oh, CS Park, S Park, JM Yang, JH Kim, HS Kim, DT Shima, JH Kim, SH Hong, YH Cho, YH Kim, JB Park, GY Koh, YS Ju, HK Lee, S Lee, I Kim
    J. Exp. Med., 2018-02-14;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  21. CCL3 augments tumor rejection and enhances CD8+T cell infiltration through NK and CD103+dendritic cell recruitment via IFN?
    Authors: F Allen, ID Bobanga, P Rauhe, D Barkauskas, N Teich, C Tong, J Myers, AY Huang
    Oncoimmunology, 2017-11-20;7(3):e1393598.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture
  22. Functional paralysis of GM-CSF-derived bone marrow cells productively infected with ectromelia virus
    Authors: L Szulc-D?br, J Struzik, A Ostrowska, M Guzera, FN Toka, M Bossowska-, MM Giery?ska, A Winnicka, Z Nowak, MG Niemia?tow
    PLoS ONE, 2017-06-12;12(6):e0179166.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Pharmacologic targeting ERK1/2 attenuates the development and progression of hyperuricemic nephropathy in rats
    Authors: N Liu, L Xu, Y Shi, L Fang, H Gu, H Wang, X Ding, S Zhuang
    Oncotarget, 2017-05-16;8(20):33807-33826.
    Species: Rat
    Sample Types: Tissue Culture Supernates
  24. Ccl5 establishes an autocrine high-grade glioma growth regulatory circuit critical for mesenchymal glioblastoma survival
    Authors: Y Pan, LJ Smithson, Y Ma, D Hambardzum, DH Gutmann
    Oncotarget, 2017-05-16;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Soluble Fn14 Is Detected and Elevated in Mouse and Human Kidney Disease
    PLoS ONE, 2016-05-12;11(5):e0155368.
    Species: Mouse
    Sample Types: Urine
  26. Dendritic cells induce Th2-mediated airway inflammatory responses to house dust mite via DNA-dependent protein kinase.
    Authors: Mishra, Amarjit, Brown, Alexandr, Yao, Xianglan, Yang, Shutong, Park, Sung-Jun, Liu, Chengyu, Dagur, Pradeep, McCoy, J Philip, Keeran, Karen J, Nugent, Gayle Z, Jeffries, Kenneth, Qu, Xuan, Yu, Zu-Xi, Levine, Stewart, Chung, Jay H
    Nat Commun, 2015-02-18;6(0):6224.
    Species: Mouse
    Sample Types: BALF
  27. Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
    Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
    Nat Commun, 2014-09-02;5(0):4715.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. Prostaglandin E2 negatively regulates the production of inflammatory cytokines/chemokines and IL-17 in visceral leishmaniasis.
    Authors: Saha A, Biswas A, Srivastav S, Mukherjee M, Das P, Ukil A
    J Immunol, 2014-07-21;193(5):2330-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. Activation of A1-adenosine receptors promotes leukocyte recruitment to the lung and attenuates acute lung injury in mice infected with influenza A/WSN/33 (H1N1) virus.
    Authors: Aeffner F, Woods P, Davis I
    J Virol, 2014-06-25;88(17):10214-27.
    Species: Mouse
    Sample Types: BALF
  30. Astrocytic TGF-beta signaling limits inflammation and reduces neuronal damage during central nervous system Toxoplasma infection.
    Authors: Cekanaviciute E, Dietrich H, Axtell R, Williams A, Egusquiza R, Wai K, Koshy A, Buckwalter M
    J Immunol, 2014-05-23;193(1):139-49.
    Species: Mouse
    Sample Types: Tissue Homogenates
  31. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy.
    Authors: Brenndorfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M
    J Immunol, 2014-01-17;192(4):1671-80.
    Species: Mouse
    Sample Types: Cell Lysates
  32. Pharmacologic inhibition of S-nitrosoglutathione reductase protects against experimental asthma in BALB/c mice through attenuation of both bronchoconstriction and inflammation.
    Authors: Blonder J, Mutka S, Sun X, Qiu J, Green L, Mehra N, Boyanapalli R, Suniga M, Look K, Delany C, Richards J, Looker D, Scoggin C, Rosenthal G
    BMC Pulm Med, 2014-01-10;14(0):3.
    Species: Mouse
    Sample Types: Plasma
  33. Ifit2 deficiency results in uncontrolled neurotropic coronavirus replication and enhanced encephalitis via impaired alpha/beta interferon induction in macrophages.
    Authors: Butchi N, Hinton D, Stohlman S, Kapil P, Fensterl V, Sen G, Bergmann C
    J Virol, 2013-11-06;88(2):1051-64.
    Species: Mouse
    Sample Types: Tissue Homogenates
  34. Sirt2 suppresses inflammatory responses in collagen-induced arthritis.
    Authors: Lin J, Sun B, Jiang C, Hong H, Zheng Y
    Biochem Biophys Res Commun, 2013-11-06;441(4):897-903.
    Species: Mouse
    Sample Types: Serum
  35. Essential role of CD11a in CD8+ T-cell accumulation and activation in adipose tissue.
    Authors: Jiang E, Perrard X, Yang D, Khan I, Perrard J, Smith C, Ballantyne C, Wu H
    Arterioscler Thromb Vasc Biol, 2013-10-24;34(1):34-43.
    Species: Mouse
    Sample Types: Tissue Homogenates
  36. Interferon gamma-induced intratumoral expression of CXCL9 alters the local distribution of T cells following immunotherapy with
    Authors: Guirnalda P, Wood L, Goenka R, Crespo J, Paterson Y
    Oncoimmunology, 2013-07-23;2(8):e25752.
    Species: Mouse
    Sample Types: Tissue Homogenates
  37. Potential roles of CCR5(+) CCR6(+) dendritic cells induced by nasal ovalbumin plus Flt3 ligand expressing adenovirus for mucosal IgA responses.
    Authors: Fukuyama Y, Tokuhara D, Sekine S, Aso K, Kataoka K, Davydova J, Yamamoto M, Gilbert R, Tokuhara Y, Fujihashi K, Kunisawa J, Yuki Y, Kiyono H, McGhee J, Fujihashi K
    PLoS ONE, 2013-04-02;8(4):e60453.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. Liposomal lipopolysaccharide initiates TRIF-dependent signaling pathway independent of CD14.
    Authors: Watanabe S, Kumazawa Y, Inoue J
    PLoS ONE, 2013-04-02;8(4):e60078.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells.
    Authors: Guo Z, Wen Z, Qin A, Zhou Y, Liao Z, Liu Z, Liang Y, Ren T, Xu L
    J Immunol, 2013-03-20;190(8):4337-48.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  40. Intrarenal resistance index for the assessment of acute renal injury in a rat liver transplantation model.
    Authors: Kong H, Chen F, He Y, Wu L, Wang L, Zhu S, Zheng S
    BMC Nephrol, 2013-03-02;14(0):55.
    Species: Rat
    Sample Types: Serum
  41. Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
    J. Thromb. Haemost., 2012-08-01;10(8):1616-23.
    Species: Mouse
    Sample Types: Plasma
  42. TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity.
    Authors: Chen K, Chang C, Huang C, Lin C, Lin W, Lo Y, Yang C, Hsing E, Chen L, Shih S, Shiau A, Lei H, Tan T, Ling P
    J Biol Chem, 2012-07-25;287(38):32216-21.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. Cutting edge: Divergent cell-specific functions of MyD88 for inflammatory responses and organ injury in septic peritonitis.
    Authors: Gais P, Reim D, Jusek G, Rossmann-Bloeck T, Weighardt H, Pfeffer K, Altmayr F, Janssen KP, Holzmann B
    J. Immunol., 2012-05-14;188(12):5833-7.
    Species: Mouse
    Sample Types: Plasma
  44. Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease.
    Authors: Gottfries J, Melgar S, Michaelsson E
    PLoS ONE, 2012-01-18;7(1):e30005.
    Species: Mouse
    Sample Types: Tissue Homogenates
  45. IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
    Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
    J. Clin. Invest., 2011-09-19;121(10):3846-59.
    Species: Mouse
    Sample Types: Tissue Homogenates
  46. Monocyte-derived dendritic cell recruitment and allergic T(H)2 responses after exposure to diesel particles are CCR2 dependent.
    Authors: Provoost S, Maes T, Joos GF, Tournoy KG
    J. Allergy Clin. Immunol., 2011-09-08;129(2):483-91.
    Species: Mouse
    Sample Types: BALF
  47. Improved host defense against septic peritonitis in mice lacking MyD88 and TRIF is linked to a normal interferon response.
    Authors: Reim D, Rossmann-Bloeck T, Jusek G, Prazeres da Costa O, Holzmann B
    J. Leukoc. Biol., 2011-05-31;90(3):613-20.
    Species: Mouse
    Sample Types: Plasma
  48. IL-33-activated dendritic cells are critical for allergic airway inflammation.
    Authors: Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B
    Eur. J. Immunol., 2011-05-25;41(6):1675-86.
    Species: Mouse
    Sample Types: BALF
  49. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
    Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
    Am. J. Pathol., 2011-05-10;179(1):302-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
  50. Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
    Authors: Wang H, Cheng F
    J. Immunol., 2011-03-02;186(0):3986.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  51. Production and functions of IL-33 in the central nervous system.
    Authors: Yasuoka S, Kawanokuchi J, Parajuli B, Jin S, Doi Y, Noda M, Sonobe Y, Takeuchi H, Mizuno T, Suzumura A
    Brain Res., 2011-02-22;1385(0):8-17.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  52. The adaptor molecule CARD9 is essential for tuberculosis control.
    Authors: Dorhoi A, Desel C, Yeremeev V, Pradl L, Brinkmann V, Mollenkopf HJ, Hanke K, Gross O, Ruland J, Kaufmann SH
    J. Exp. Med., 2010-03-29;207(4):777-92.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  53. Combined inhibition of janus kinase 1/2 for the treatment of JAK2V617F-driven neoplasms: selective effects on mutant cells and improvements in measures of disease severity.
    Authors: Liu PC, Caulder E, Li J, Waeltz P, Margulis A, Wynn R, Becker-Pasha M, Li Y, Crowgey E, Hollis G, Haley P, Sparks RB, Combs AP, Rodgers JD, Burn TC, Vaddi K, Fridman JS
    Clin. Cancer Res., 2009-11-03;15(22):6891-900.
    Species: Mouse
    Sample Types: Plasma
  54. CCR5 is involved in resolution of inflammation in proteoglycan-induced arthritis.
    Authors: Doodes PD, Cao Y, Hamel KM, Wang Y, Rodeghero RL, Kobezda T, Finnegan A
    Arthritis Rheum., 2009-10-01;60(10):2945-53.
    Species: Mouse
    Sample Types: Serum
  55. Acid sphingomyelinase is a key regulator of cytotoxic granule secretion by primary T lymphocytes.
    Authors: Herz J, Pardo J, Kashkar H, Schramm M, Kuzmenkina E, Bos E, Wiegmann K, Wallich R, Peters PJ, Herzig S, Schmelzer E, Kronke M, Simon MM, Utermohlen O
    Nat. Immunol., 2009-06-14;10(7):761-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production.
    Authors: Gueders MM, Paulissen G, Crahay C, Quesada-Calvo F, Hacha J, Van Hove C, Tournoy K, Louis R, Foidart JM, Noel A, Cataldo DD
    Inflamm. Res., 2009-06-09;58(12):845-54.
    Species: Mouse
    Sample Types: BALF
  57. Mortality following peripheral infection with tick-borne encephalitis virus results from a combination of central nervous system pathology, systemic inflammatory and stress responses.
    Authors: Hayasaka D, Nagata N, Fujii Y, Hasegawa H, Sata T, Suzuki R, Gould EA, Takashima I, Koike S
    Virology, 2009-05-24;390(1):139-50.
    Species: Mouse
    Sample Types: Serum
  58. Disruption of interleukin-1 signaling improves the quality of wound healing.
    Authors: Thomay AA, Daley JM, Sabo E, Worth PJ, Shelton LJ, Harty MW, Reichner JS, Albina JE
    Am. J. Pathol., 2009-04-23;174(6):2129-36.
    Species: Mouse
    Sample Types: Tissue Secretion
  59. Role of the heparan sulfate proteoglycan syndecan-1 (CD138) in delayed-type hypersensitivity.
    Authors: Kharabi Masouleh B, ten Dam GB, Wild MK, Seelige R, van der Vlag J, Rops AL, Echtermeyer FG, Vestweber D, van Kuppevelt TH, Kiesel L, Gotte M
    J. Immunol., 2009-04-15;182(8):4985-93.
    Species: Mouse
    Sample Types: Tissue Homogenates
  60. A severe deficiency of coagulation factor VIIa results in attenuation of the asthmatic response in mice.
    Authors: Shinagawa K, Ploplis VA, Castellino FJ
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-03-13;296(5):L763-70.
    Species: Mouse
    Sample Types: BALF
  61. Intravenous tolerance modulates macrophage classical activation and antigen presentation in experimental autoimmune encephalomyelitis.
    Authors: Li H, Ciric B, Yang J, Xu H, Fitzgerald DC, Elbehi M, Fonseca-Kelly Z, Yu S, Zhang GX, Rostami A
    J. Neuroimmunol., 2009-02-01;208(1):54-60.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  62. Deficiency of immunophilin FKBP52 promotes endometriosis.
    Authors: Hirota Y, Tranguch S, Daikoku T, Hasegawa A, Osuga Y, Taketani Y, Dey SK
    Am. J. Pathol., 2008-11-06;173(6):1747-57.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  63. A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
    Authors: Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
    Arthritis Rheum., 2008-11-01;58(11):3510-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  64. TLR-2 and IL-17A in chitin-induced macrophage activation and acute inflammation.
    Authors: Da Silva CA, Hartl D, Liu W, Lee CG, Elias JA
    J. Immunol., 2008-09-15;181(6):4279-86.
    Species: Mouse
    Sample Types: BALF
  65. Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
    Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
    J. Exp. Med., 2008-08-11;205(9):2075-84.
    Species: Mouse
    Sample Types: Serum
  66. Expression of the chemokine antagonist vMIP II using a non-viral vector can prolong corneal allograft survival.
    Authors: Pillai RG, Beutelspacher SC, Larkin DF, George AJ
    Transplantation, 2008-06-15;85(11):1640-7.
    Species: Mouse
    Sample Types: Tissue Homogenates
  67. The cannabinoid receptor-1 antagonist rimonabant inhibits platelet activation and reduces pro-inflammatory chemokines and leukocytes in Zucker rats.
    Authors: Schafer A, Pfrang J, Neumuller J, Fiedler S, Ertl G, Bauersachs J
    Br. J. Pharmacol., 2008-05-12;154(5):1047-54.
    Species: Rat
    Sample Types: Serum
  68. Tyrosine phosphatase SHP-1 in oxidative stress and development of allergic airway inflammation.
    Authors: Cho YS, Oh SY, Zhu Z
    Am. J. Respir. Cell Mol. Biol., 2008-04-25;39(4):412-9.
    Species: Mouse
    Sample Types: BALF
  69. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
    Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
    Am. J. Pathol., 2008-04-10;172(5):1209-21.
    Species: Mouse
    Sample Types: Tissue Homogenates
  70. Synthetic chemerin-derived peptides suppress inflammation through ChemR23.
    Authors: Cash JL, Hart R, Russ A, Dixon JP, Colledge WH, Doran J, Hendrick AG, Carlton MB, Greaves DR
    J. Exp. Med., 2008-04-07;205(4):767-75.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  71. Regulatory T cells control dendritic cell/NK cell cross-talk in lymph nodes at the steady state by inhibiting CD4+ self-reactive T cells.
    Authors: Terme M, Chaput N, Combadiere B, Ma A, Ohteki T, Zitvogel L
    J. Immunol., 2008-04-01;180(7):4679-86.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  72. Neutrophil chemokines KC and macrophage-inflammatory protein-2 are newly synthesized by tissue macrophages using distinct TLR signaling pathways.
    Authors: De Filippo K, Henderson RB, Laschinger M, Hogg N
    J. Immunol., 2008-03-15;180(6):4308-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  73. Mouse models of rhinovirus-induced disease and exacerbation of allergic airway inflammation.
    Authors: Bartlett NW, Walton RP, Edwards MR, Aniscenko J, Caramori G, Zhu J, Glanville N, Choy KJ, Jourdan P, Burnet J, Tuthill TJ, Pedrick MS, Hurle MJ, Plumpton C, Sharp NA, Bussell JN, Swallow DM, Schwarze J, Guy B, Almond JW, Jeffery PK, Lloyd CM, Papi A, Killington RA, Rowlands DJ, Blair ED, Clarke NJ, Johnston SL
    Nat. Med., 2008-02-03;14(2):199-204.
    Species: Mouse
    Sample Types: BALF
  74. Liposomal retinoic acids modulate asthma manifestations in mice.
    Authors: Maret M, Ruffie C, Periquet B, Campo AM, Menevret M, Phelep A, Dziewiszek K, Druilhe A, Pretolani M
    J. Nutr., 2007-12-01;137(12):2730-6.
    Species: Mouse
    Sample Types: BALF
  75. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007-05-15;245(2):70-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  76. Chemokine receptor CCR2 but not CCR5 or CCR6 mediates the increase in pulmonary dendritic cells during allergic airway inflammation.
    Authors: Robays LJ, Maes T, Lebecque S, Lira SA, Kuziel WA, Brusselle GG, Joos GF, Vermaelen KV
    J. Immunol., 2007-04-15;178(8):5305-11.
    Species: Mouse
    Sample Types: BALF
  77. Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper.
    Authors: Hamdi H, Godot V, Maillot MC, Prejean MV, Cohen N, Krzysiek R, Lemoine FM, Zou W, Emilie D
    Blood, 2007-03-13;110(1):211-9.
  78. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006-11-20;203(12):2763-77.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  79. Local production of chemokines and prostaglandin E2 in the acute, chronic and recovery phase of murine experimental colitis.
    Authors: Drmotova M, Rehnstrom E, Jansson L
    Cytokine, 2006-11-07;35(5):275-83.
    Species: Mouse
    Sample Types: Tissue Homogenates
  80. Contribution of T cell subsets to the pathophysiology of Pneumocystis-related immunorestitution disease.
    Authors: Bhagwat SP, Gigliotti F, Xu H, Wright TW
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006-08-04;291(6):L1256-66.
    Species: Mouse
    Sample Types: BALF
  81. Obligatory role for interleukin-13 in obstructive lesion development in airway allografts.
    Authors: Lama VN, Harada H, Badri LN, Flint A, Hogaboam CM, McKenzie A, Martinez FJ, Toews GB, Moore BB, Pinsky DJ
    Am. J. Pathol., 2006-07-01;169(1):47-60.
    Species: Mouse
    Sample Types: Cell Lysates
  82. Organ-specific role of MyD88 for gene regulation during polymicrobial peritonitis.
    Authors: Weighardt H, Mages J, Jusek G, Kaiser-Moore S, Lang R, Holzmann B
    Infect. Immun., 2006-06-01;74(6):3618-32.
    Species: Mouse
    Sample Types: Tissue Homogenates
  83. Immunosuppressant triptolide inhibits dendritic cell-mediated chemoattraction of neutrophils and T cells through inhibiting Stat3 phosphorylation and NF-kappaB activation.
    Authors: Liu Q, Chen T, Chen G, Li N, Wang J, Ma P, Cao X
    Biochem. Biophys. Res. Commun., 2006-05-12;345(3):1122-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  84. Inhalation tolerance is induced selectively in thoracic lymph nodes but executed pervasively at distant mucosal and nonmucosal tissues.
    Authors: Alvarez D, Swirski FK, Yang TC, Fattouh R, Croitoru K, Bramson JL, Stampfli MR, Jordana M
    J. Immunol., 2006-02-15;176(4):2568-80.
    Species: Mouse
    Sample Types: Serum
  85. TWEAK/Fn14 interaction regulates RANTES production, BMP-2-induced differentiation, and RANKL expression in mouse osteoblastic MC3T3-E1 cells.
    Authors: Ando T, Ichikawa J, Wako M, Hatsushika K, Watanabe Y, Sakuma M, Tasaka K, Ogawa H, Hamada Y, Yagita H, Nakao A
    Arthritis Res. Ther., 2006-01-01;8(5):R146.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  86. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
    Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
    Blood, 2005-11-03;107(5):2112-22.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  87. Interleukin-10 aerosol reduces proinflammatory mediators in bronchoalveolar fluid of endotoxemic rat.
    Authors: Hofstetter C, Flondor M, Hoegl S, Muhl H, Zwissler B
    Crit. Care Med., 2005-10-01;33(10):2317-22.
    Species: Rat
    Sample Types: BALF
  88. Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice.
    Authors: Trabattoni D, Clivio A, Bray DH, Bhagat L, Beltrami S, Maffeis G, Cesana E, Lowry P, Lissoni F, Kandimalla ER, Sullivan T, Agrawal S, Bartholomew R, Clerici M
    Vaccine, 2005-09-22;24(9):1470-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  89. A protective role of locally administered immunostimulatory CpG oligodeoxynucleotide in a mouse model of genital herpes infection.
    Authors: Harandi AM, Eriksson K, Holmgren J
    J. Virol., 2003-01-01;77(2):953-62.
    Species: Mouse
    Sample Types: Serum
  90. Resident cell chemokine expression serves as the major mechanism for leukocyte recruitment during local inflammation.
    Authors: Garcia-Ramallo E, Marques T, Prats N, Beleta J, Kunkel SL, Godessart N
    J. Immunol., 2002-12-01;169(11):6467-73.
    Species: Mouse
    Sample Types: Air Pouch Exudate
  91. Role of the autocrine chemokines MIP-1alpha and MIP-1beta in the metastatic behavior of murine T cell lymphoma.
    Authors: Menten P, Saccani A, Dillen C, Wuyts A, Struyf S, Proost P, Mantovani A, Wang JM, Van Damme J
    J. Leukoc. Biol., 2002-10-01;72(4):780-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  92. NKT cell-derived RANTES recruits APCs and CD8+ T cells to the spleen during the generation of regulatory T cells in tolerance.
    Authors: Faunce DE, Stein-Streilein J
    J. Immunol., 2002-07-01;169(1):31-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  93. Immunostimulatory DNA-based vaccines elicit multifaceted immune responses against HIV at systemic and mucosal sites.
    Authors: Horner AA, Datta SK, Takabayashi K, Belyakov IM, Cinman N, Nguyen MD, Van Uden JH, Berzofsky JA
    J. Immunol., 2001-08-01;167(3):1584-91.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  94. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
    Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
    Eur. J. Immunol., 2001-07-01;31(7):2104-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  95. Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
    Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
    J. Immunol., 2000-03-01;164(5):2650-9.
    Species: Rat
    Sample Types: BALF

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Mouse/Rat CCL5/RANTES Quantikine ELISA Kit
By Anonymous on 06/08/2022
Sample Tested: Serum-free Cell Culture Media