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Mouse/Rat CD34 Antibody

Catalog #: AF6518
8 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF6518
AF6518-SP
Detection of Mouse and Rat CD34 by Western Blot.
2 Images
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat CD34 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat CD34 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant porcine CD34 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD34
Thr35-Thr287
Accession # Q64314
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat CD34 antibody by Western Blot. View Larger

Detection of Mouse and Rat CD34 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue and rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6518) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD34 at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of CD34 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CD34 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6518, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

Mouse and rat CD34 is a 80 -100 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, CD34+ stem cells and renal mesangial cells. Mature mouse CD34 is a 348 amino acid (aa) type I transmembrane protein (aa 35-382). It contains a 253 aa extracellular region (aa 35-287), and a 74 aa cytoplasmic domain that undergoes Tyr phosphorylation. Mouse and rat CD34 also is likely to undergo membrane cleavage that will generate a 54 kDa soluble form. Over aa 35-287, mouse CD34 shares 57% and 82% aa identity with human and rat Cd34, respectively.

Entrez Gene IDs
947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
Alternate Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1

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Citations for Mouse/Rat CD34 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Photobiostimulation conjugated with stem cells or their secretome for temporomandibular joint arthritis in a rat model
    Authors: El-Qashty, R;Elkashty, OA;Hany, E;
    BMC oral health
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Ctyometry
  2. Combination human umbilical cord perivascular and endothelial colony forming cell therapy for ischemic cardiac injury
    Authors: Iqbal F, Johnston A, Wyse B et al.
    npj Regenerative Medicine
  3. Adipose-Derived Stem Cells from Type 2 Diabetic Rats Retain Positive Effects in a Rat Model of Erectile Dysfunction
    Authors: Marlene Louise Quaade, Pratibha Dhumale, Simon Gabriel Comerma Steffensen, Hans Christian Beck, Eva Bang Harvald, Charlotte Harken Jensen et al.
    International Journal of Molecular Sciences
  4. The Effect of Electronic-Cigarette Vaping on Cardiac Function and Angiogenesis in Mice
    Authors: H Shi, X Fan, A Horton, ST Haller, DJ Kennedy, IT Schiefer, L Dworkin, CJ Cooper, J Tian
    Sci Rep, 2019-03-11;9(1):4085.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts
    Authors: R El-Habta, M Sloniecka, PJ Kingham, LJ Backman
    Stem Cell Res Ther, 2018-12-20;9(1):352.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Fibrocyte migration, differentiation and apoptosis during the corneal wound healing response to injury
    Authors: L Lassance, GK Marino, CS Medeiros, S Thangavadi, SE Wilson
    Exp. Eye Res., 2018-02-24;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Immunomodulatory effects of adipose tissue-derived stem cells on elastin scaffold remodeling in diabetes
    Authors: James P. Chow, Dan T. Simionescu, Anna L. Carter, Agneta Simionescu
    Tissue Engineering and Regenerative Medicine
  8. Synaptic plasticity and sensory-motor improvement following fibrin sealant dorsal root reimplantation and mononuclear cell therapy
    Authors: Suzana U. Benitez, Roberta Barbizan, Aline B. Spejo, Rui S. Ferreira, Benedito Barraviera, Alfredo M. Góes et al.
    Frontiers in Neuroanatomy

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Mouse/Rat CD34 Antibody
By Anonymous on 05/15/2018
Application: IHC Sample Tested: Eye lens tissue Species: Rabbit