Home / DLL1 / Mouse/Rat DLL1 Antibody

Mouse/Rat DLL1 Antibody

Catalog #: AF5026
10 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5026
AF5026-SP
DLL1 in Embryonic Mouse Kidney.
1 Image
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat DLL1 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat DLL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) DLL1 and less than 1% cross-reactivity with rhDLL3, rhDLL4, and recombinant mouse DLL4 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse DLL1
Ser22-Gln516
Accession # Q61483
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse DLL1 Fc Chimera (Catalog # 5026-DL)
Recombinant Rat DLL1 Fc Chimera (Catalog # 3970-DL)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry DLL1 antibody in Embryonic Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

DLL1 in Embryonic Mouse Kidney. DLL1 was detected in immersion fixed frozen sections of embryonic mouse kidney (E13.5) using 10 µg/mL Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5026) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90‑100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature mouse DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, mouse DLL1 shares 91% and 95% aa sequence identity with human and rat DLL1, respectively. It shares 26%, 35%, and 51% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment, released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells (2, 3). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma -secretase, enabling the cytoplasmic domain to migrate to the nucleus (4). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (5). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (6‑12). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (13). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (14‑15).

References
  1. Bettenhausen, B. et al. (1995) Development 121:2407.
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
  3. Karanu, F.N. et al. (2001) Blood 97:1960.
  4. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  5. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
  6. Takahashi, Y. et al. (2003) Development 130:4259.
  7. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  8. Kiernan, A.E. et al. (2005) Development 132:4353.
  9. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  10. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  11. Shimojo, H. et al. (2008) Neuron 58:52.
  12. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. 104:537.
  13. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  14. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
  15. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.
Long Name
Delta-like 1
Entrez Gene IDs
28514 (Human); 13388 (Mouse); 84010 (Rat)
Alternate Names
delta (Drosophila)-like 1; Delta 1; Delta; Delta1; delta-like 1 (Drosophila); delta-like protein 1; DL1; DLL1; Drosophila Delta homolog 1; H-Delta-1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse/Rat DLL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements
    Authors: Gi Bum Kim, David Rincon Fernandez Rincon Fernandez Pacheco, David Saxon, Amy Yang, Sara Sabet, Marina Dutra-Clarke et al.
    Cell
  2. Distinct expression patterns of Notch ligands, Dll1 and Dll4, in normal and inflamed mice intestine
    Authors: Hiromichi Shimizu, Ryuichi Okamoto, Go Ito, Satoru Fujii, Toru Nakata, Kohei Suzuki et al.
    PeerJ
  3. Intermediate progenitors support migration of neural stem cells into dentate gyrus outer neurogenic niches
    Authors: Branden R Nelson, Rebecca D Hodge, Ray AM Daza, Prem Prakash Tripathi, Sebastian J Arnold, Kathleen J Millen et al.
    eLife
  4. Temperature sensitivity of Notch signaling underlies species-specific developmental plasticity and robustness in amniote brains
    Authors: T Nomura, K Nagao, R Shirai, H Gotoh, M Umeda, K Ono
    Nature Communications, 2022-01-10;13(1):96.
    Species: Chicken
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube
    Authors: S Ofek, S Wiszniak, S Kagan, M Tondl, Q Schwarz, C Kalcheim
    Bmc Biology, 2021-04-23;19(1):84.
    Species: Mouse
    Sample Types: Embryo
    Applications: IHC
  6. Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration
    Authors: S Wiszniak, Q Schwarz
    BMC Neurosci, 2019-04-29;20(1):21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  7. Dll1 Marks Cells of Origin of Ras-Induced Cancer in Mouse Squamous Epithelia
    Authors: E Vasilaki, Z Kanaki, DJ Stravopodi, A Klinakis
    Transl Oncol, 2018-08-03;11(5):1213-1219.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine.
    Authors: Toru Nakata, Hiromichi Shimizu, Sayaka Nagata, Go Ito, Satoru Fujii, Kohei Suzuki, Ami Kawamoto, Fumiaki Ishibashi, Reiko Kuno, Sho Anzai, Tatsuro Murano, Tomohiro Mizutani, Shigeru Oshima, Kiichiro Tsuchiya, Tetsuya Nakamura, Katsuto Hozumi, Mamoru Watanabe, Ryuichi Okamoto
    Data in Brief, 2016-12-29;0(0):2352-3409.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Notch Coordinates Periodontal Ligament Maturation through Regulating Lamin A
    Authors: B.J. Denes, C. Bolton, C.S. Illsley, W.L. Kok, J.V. Walker, A. Poetsch et al.
    Journal of Dental Research
  10. Requirement of G alpha q /G alpha 11 Signaling in the Preservation of Mouse Intestinal Epithelial Homeostasis
    Authors: Noboru Watanabe, Hirosato Mashima, Kouichi Miura, Takashi Goto, Makoto Yoshida, Akiteru Goto et al.
    Cellular and Molecular Gastroenterology and Hepatology

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse/Rat DLL1 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse/Rat DLL1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse/Rat DLL1 Antibody
By Anonymous on 08/06/2021
Application: WB
Reason for Rating: It worked very good for western blot. Sample Tested: Brain (cortex) tissue,brain cortex Species: Mouse

1:500 dilution is good.

Western Blot Mouse/Rat DLL1 Antibody AF5026