Mouse/Rat DLL1 Antibody

DLL1 in Embryonic Mouse Kidney. DLL1 was detected in immersion fixed frozen sections of embryonic mouse kidney (E13.5) using 10 µg/mL Sheep Anti-Mouse/Rat DLL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5026) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Mouse DLL1 by Immunohistochemistry Dll1 and Dll4 are expressed in distinct patterns in the mice gastrointestinal epithelium.Immunohistochemistry of Dll1 and Dll4 were performed using mice small intestinal (A, B) and colonic tissues (D, E). Within the small intestinal epithelium, staining by anti-Dll1 antibody showed positive cells exclusively within the crypt (A, B), whereas staining by anti-Dll4 antibody showed positive cells both in the crypt and in the villi. Quantification of Dll1+ve and Dll4+ve intestinal epithelial cells (IECs) showed that Dll1+ve IECs are predominantly found within the crypt, whereas Dll4+ve IECs are found mostly in the villi (C). In the colonic epithelium, staining by anti-Dll1 antibody showed positive cells exclusively within the lower part of the crypt (D, E), whereas staining by anti-Dll4 antibody showed positive cells both in the lower- and upper part of the crypt, including the surface epithelium. Quantification of Dll1+ve and Dll4+ve IECs showed that Dll1+ve IECs are found exclusively within the lower half of the crypt, whereas Dll4+ve IECs are found both in the lower and upper region of the crypt at a comparable frequency (F). Negative staining of non-immunized isotype antibodies (Control Ab) confirmed the specific staining of Dll1 (A) and Dll4 (D). Scale bar represents 20 µm. Quantitative data are shown as mean ∓ SD of triplicate experiments (n = 3). ∗ indicates P < 0.05 as determined by Welch’s t-test. N.S. indicates not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse DLL1 by Immunohistochemistry Dll1+ve or Dll4+ve IECs constitute the intestinal stem cell niche.Double immunostaining of Dll1 or Dll4 with a stem-cell niche cell marker, Lysozyme or c-kit, was performed in the mice small intestine and in the colon, respectively. (A) Immunostaining of Dll4 (green) with Lysozyme (red) shows that Dll4 is expressed in Paneth cells of the small intestine (yellow arrowhead). Scale bar represents 20 µm. (B) Double immunostaining of Dll1 (green) and c-kit (red) shows that Dll1+ve colonic IECs mostly co-express c-kit (yellow arrowhead). Also, double immunostaining of Dll4 (green) and c-kit (red) showed that a distinct population of Dll4+ve cells co-express c-kit (yellow arrowhead). Scale bar represents 20 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse DLL1 by Immunohistochemistry Dll4+ve cells dominate the colonic crypts of the DSS-colitis mice.Immunohistochemical analysis of colonic tissues that prepared from DSS-colitis mice is shown. (A) Immunostaining of Dll1&Dll4 using the inflamed colonic tissue of the DSS-colitis mice at day 10 (Colitis), or the corresponding tissue of the control mice (Control). In the inflamed colon, Dll1+ve IECs (green, upper panel) rarely found within the crypts (yellow arrowhead). In sharp contrast, Dll4+ve IECs frequently found, which dominated the entire crypt&also the surface epithelium. A magnified view of the area marked with a white square is shown in the right-end panel. Scale bar represents 50 µm. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse DLL1 by Immunohistochemistry Both Dll1+ve and Dll4+ve IECs are mostly post-mitotic in the small intestine and in the colon.Double immunostaining of Dll1 or Dll4 (green) with the proliferation cell marker, Ki67 (red), shows that both Dll1+ve and Dll4+ve IECs are mostly post-mitotic in the small intestine (A) and in the colon (B). However, a small number of Dll1+ve or Dll4+ve IECs that co-express Ki67 can be found (yellow arrowhead). Scale bar represents 20 µm. These data were acquired by confocal microscopy (FV10i). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse DLL1 by Immunohistochemistry Both Dll1+ve and Dll4+ve IECs are mostly post-mitotic in the small intestine and in the colon.Double immunostaining of Dll1 or Dll4 (green) with the proliferation cell marker, Ki67 (red), shows that both Dll1+ve and Dll4+ve IECs are mostly post-mitotic in the small intestine (A) and in the colon (B). However, a small number of Dll1+ve or Dll4+ve IECs that co-express Ki67 can be found (yellow arrowhead). Scale bar represents 20 µm. These data were acquired by confocal microscopy (FV10i). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24860699), licensed under a CC-BY license. Not internally tested by R&D Systems.