Mouse/Rat Follistatin-like 1/FSTL1 Antibody

Catalog #: MAB17381
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB17381
MAB17381-SP

Follistatin-like 1/FSTL1 in PC‑12 Rat Cell Line.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Follistatin-like 1/FSTL1 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse Follistatin-like 1/FSTL1 in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 228208

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Follistatin-like 1/FSTL1
Glu19-Ile306
Accession # NP_032073

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Follistatin-like 1/FSTL1 in PC‑12 Rat Cell Line. Follistatin-like 1/FSTL1 was detected in immersion fixed PC-12 rat adrenal pheochromocytoma cell line using Rat Anti-Mouse/Rat Follistatin-like 1/FSTL1 Mono-clonal Antibody (Catalog # MAB17381) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Follistatin-like 1/FSTL1

Follistatin-Like 1 (FSTL1), also known as FSTL1 and follistatin-related protein (FRP), is a secreted protein that contains a follistatin-like domain, a Kazal-like domain and a von Willebrand factor C domain. Human FSTL1 shares 92% amino acid sequence identity with the mouse FSTL1, also known as TSC-36.

Entrez Gene IDs

11167 (Human); 14314 (Mouse); 79210 (Rat)

Alternate Names

Flik; FLJ50214; Follistatin-like 1; Follistatin-like protein 1; follistatin-related protein 1; Follistatin-related Protein; FRP; FRPFLJ52277; FSL1; FSTL1; TSC-36

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse/Rat Follistatin-like 1/FSTL1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Follistatin like-1 aggravates silica-induced mouse lung injury
Authors: Y Fang, S Zhang, X Li, F Jiang, Q Ye, W Ning
Sci Rep, 2017-03-24;7(1):399.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Epicardial FSTL1 reconstitution regenerates the adult mammalian heart.
Authors: Wei K, Serpooshan V, Hurtado C, Diez-Cunado M, Zhao M, Maruyama S, Zhu W, Fajardo G, Noseda M, Nakamura K, Tian X, Liu Q, Wang A, Matsuura Y, Bushway P, Cai W, Savchenko A, Mahmoudi M, Schneider M, van den Hoff M, Butte M, Yang P, Walsh K, Zhou B, Bernstein D, Mercola M, Ruiz-Lozano P
Nature, 2015-09-16;525(7570):479-85.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P