Mouse/Rat HIN-1/SCGB3A1 Antibody

Catalog #: AF2954
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2954
AF2954-SP

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat HIN-1/SCGB3A1 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse HIN‑1/SCGB3A1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human HIN-1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse HIN‑1/SCGB3A1
Phe22-Gly104
Accession # Q920D7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat HIN-1 by Western Blot. Western blot shows lysates of rat lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat HIN-1/SCGB3A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2954) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIN-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIN-1/SCGB3A1

HIN-1, also known as PnSP‑2, LuLeu‑2, UGRP‑2 and SCGB3A1, is an 10 kDa member of the secretoglobulin family of molecules. It is synthesized as a 104 amino acid (aa) precursor that contains a 21 aa signal sequence and an 83 aa mature region. There are no potential N‑linked glycosylation sites. Based on other members of the family, HIN‑1 is likely to exist as a disulfide‑linked homodimer. The molecule is found in mammary and upper airway epithelium. No receptor for HIN‑1 has been identified. Mature mouse HIN‑1 shares 60% aa sequence identity with mature human HIN‑1.

Long Name

High in Normal 1

Entrez Gene IDs

92304 (Human); 73945 (Mouse)

Alternate Names

cytokine high in normal-1; Cytokine HIN-1; High in normal 1; HIN1; HIN-1; HIN1Pneumo secretory protein 2; LU105; PNSP2; PnSP-2; PnSP-2Uteroglobin-related protein 2; SCGB3A1; secretoglobin family 3A member 1; secretoglobin, family 3A, member 1; UGRP2; UGRP2MGC87867

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse/Rat HIN-1/SCGB3A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Neonatal Hyperoxic Exposure Persistently Alters Lung Secretoglobins and Annexin A1
Authors: Thomas M. Raffay, Morgan L. Locy, Cynthia L. Hill, Nik S. Jindal, Lynette K. Rogers, Stephen E. Welty et al.
BioMed Research International
Spatiotemporal Expression of Three Secretoglobin Proteins, SCGB1A1, SCGB3A1, and SCGB3A2, in Mouse Airway Epithelia
Authors: Xu Naizhen, Taketomo Kido, Shigetoshi Yokoyama, R. Ilona Linnoila, Shioko Kimura
Journal of Histochemistry & Cytochemistry
IFN-gamma down-regulates Secretoglobin 3A1 gene expression.
Authors: Yamada A, Suzuki D, Miyazono A, Oshima K, Kamiya A, Zhao B, Takami M, Donnelly RP, Itabe H, Yamamoto M, Kimura S, Kamijo R
Biochem. Biophys. Res. Commun., 2009-01-09;379(4):964-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot