Mouse/Rat MMP-8 Antibody

Catalog #: AF3245
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3245
AF3245-SP

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat MMP-8 Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects rat and mouse MMP-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human MMP‑8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat MMP-8
Leu21-Pro466
Accession # O88766

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Rat MMP‑8 (Catalog # 3245-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat MMP‑8 by Western Blot. Western blot shows lysates of rat lung tissue and rat spleen tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3245) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-8 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-8

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase, collagenase 2) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II, and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein, and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP-8 has a high capacity for infiltrating connective tissue and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP-8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region, and a hemopexin-like domain (1).

References

Tschesche, T. et al. (2004) Handbook of Proteolytic Enzymes (eds. A.J. Barrett et al.) p.480, Academic Press, San Diego.

Long Name

Matrix Metalloproteinase 8

Entrez Gene IDs

4317 (Human); 17394 (Mouse); 63849 (Rat)

Alternate Names

CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse/Rat MMP-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

MMP-10 Regulates Collagenolytic Activity of Alternatively Activated Resident Macrophages.
Authors: Rohani M, McMahan R, Razumova M, Hertz A, Cieslewicz M, Pun S, Regnier M, Wang Y, Birkland T, Parks W
J Invest Dermatol, 2015-04-30;135(10):2377-84.
Species: Mouse
Sample Types: Whole Tissue
Applications: Neutralization