Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit

Catalog #: MG200
10 Citations Datasheet / COA / SDS

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MG200

Mouse/Rat/Porcine/Canine IGF-II ELISA Standard Curve

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All IGF-II/IGF2 ELISAs

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Citations (10)

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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit Summary

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Serum-free Cell Culture Supernates (50 uL), Mouse Serum (10 uL), Rat Serum (10 uL), Canine Serum (10 uL), Porcine Serum (10 uL)

Sensitivity

1.87 pg/mL

Assay Range

12.5 - 800 pg/mL (Serum-free Cell Culture Supern, Mouse Serum, Rat Serum, Canine Serum, Porcine Serum)

Specificity

Natural and recombinant IGF-II.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine® Mouse/Rat/Porcine/Canine IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure IGF-II in serum-free cell culture supernates and serum. It contains E. coli-expressed recombinant IGF-II and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural IGF-II.

Recovery

The recovery of IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Mouse Serum (n=4)	105	86-124
Rat Serum (n=4)	84	78-89
Serum-free Cell Culture Media (n=4)	92	84-101

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Mouse/Rat/Porcine/Canine IGF-II ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IGF-II/IGF2

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

Long Name:

Insulin-like Growth Factor II

Entrez Gene IDs:

3481 (Human); 16002 (Mouse)

Alternate Names:

C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide and methanol, which are known to the State of California to cause cancer and reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse/Rat/Porcine/Canine IGF-II/IGF2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Tobacco-induced hyperglycemia promotes lung cancer progression via cancer cell-macrophage interaction through paracrine IGF2/IR/NPM1-driven PD-L1 expression
Authors: Jang, HJ;Min, HY;Kang, YP;Boo, HJ;Kim, J;Ahn, JH;Oh, SH;Jung, JH;Park, CS;Park, JS;Kim, SY;Lee, HY;
Nature communications
Species: Mouse
Sample Types: Cell Culture Supernates
The metabolic effects of resumption of a high fat diet after weight loss are sex dependent in mice
Authors: Guerra-Cantera, S;Frago, LM;Jiménez-Hernaiz, M;Collado-Pérez, R;Canelles, S;Ros, P;García-Piqueras, J;Pérez-Nadador, I;Barrios, V;Argente, J;Chowen, JA;
Scientific reports
Species: Mouse
Sample Types: Plasma
Local autocrine plasticity signaling in single dendritic spines by insulin-like growth factors
Authors: Tu, X;Jain, A;Parra Bueno, P;Decker, H;Liu, X;Yasuda, R;
Science advances
Species: Mouse
Sample Types: Cell Culture Supernatants
A gelatin hydrogel nonwoven fabric improves outcomes of subcutaneous islet transplantation
Authors: Kanai, N;Inagaki, A;Nakamura, Y;Imura, T;Mitsugashira, H;Saito, R;Miyagi, S;Watanabe, K;Kamei, T;Unno, M;Tabata, Y;Goto, M;
Scientific reports
Species: Mouse
Sample Types: Cell Culture Supernates
Ideal Duration of Pretreatment Using a Gelatin Hydrogel Nonwoven Fabric Prior to Subcutaneous Islet Transplantation
Authors: Saito, R;Inagaki, A;Nakamura, Y;Imura, T;Kanai, N;Mitsugashira, H;Endo, Y;Katano, T;Suzuki, S;Tokodai, K;Kamei, T;Unno, M;Watanabe, K;Tabata, Y;Goto, M;
Cell transplantation
Species: Mouse
Sample Types: Cell Culture Supernates
Development of serum-free and grain-derived-nutrient-free medium using microalga-derived nutrients and mammalian cell-secreted growth factors for sustainable cultured meat production
Authors: K Yamanaka, Y Haraguchi, H Takahashi, I Kawashima, T Shimizu
Scientific Reports, 2023-01-10;13(1):498.
Species: Bovine
Sample Types: Cell Culture Supernates.
Comparative Effects of Allulose, Fructose, and Glucose on the Small Intestine
Authors: T Suzuki, Y Sato, S Kadoya, T Takahashi, M Otomo, H Kobayashi, K Aoki, M Kantake, M Sugiyama, RP Ferraris
Nutrients, 2022-08-07;14(15):.
Species: Rat
Sample Types: Plasma
Critical timing of ACEi initiation prevents compensatory glomerular hypertrophy in the remaining single kidney
Authors: AS Naik, SQ Wang, M Chowdhury, J Aqeel, CL O'Connor, JE Wiggins, M Bitzer, RC Wiggins
Scientific Reports, 2021-10-01;11(1):19605.
Species: Rat
Sample Types: Urine
Runx2+ Niche Cells Maintain Incisor Mesenchymal Tissue Homeostasis through IGF Signaling
Authors: S Chen, J Jing, Y Yuan, J Feng, X Han, Q Wen, TV Ho, C Lee, Y Chai
Cell Rep, 2020-08-11;32(6):108007.
Species: Mouse
Sample Types: Cell Culture Supernates
Insulin-like growth factor-II overexpression accelerates parthenogenetic stem cell differentiation into cardiomyocytes and improves cardiac function after acute myocardial infarction in mice
Authors: Y Sui, W Zhang, T Tang, L Gao, T Cao, H Zhu, Q You, B Yu, T Yang
Stem Cell Res Ther, 2020-02-26;11(1):86.
Species: Mouse
Sample Types: Serum