Home / Serpin E1/PAI-1 / Mouse Serpin E1/PAI-1 DuoSet ELISA

Mouse Serpin E1/PAI-1 DuoSet ELISA

Catalog #: DY3828-05
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DY3828-05
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
DY999B: Substrate Reagent Pack
DY999: Substrate Reagent Pack
Mouse Serpin E1 / PAI-1 ELISA Standard Curve
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews
Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse Serpin E1/PAI-1 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
9.4 - 600 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse Serpin E1 / PAI-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A Mouse Serpin E1 / PAI-1 ELISA Standard Curve View Larger

Mouse Serpin E1 / PAI-1 ELISA Standard Curve

Product Datasheets

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Product Datasheet
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SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin E1/PAI-1

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Long Name:
Plasminogen Activator Inhibitor
Entrez Gene IDs:
5054 (Human); 18787 (Mouse)
Alternate Names:
Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Citations for Mouse Serpin E1/PAI-1 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Hutchinson-Gilford progeria syndrome mice display accelerated arterial thrombus formation and increased platelet reactivity
    Authors: Puspitasari, YM;Ministrini, S;Han, J;Karch, C;Prisco, F;Liberale, L;Bengs, S;Akhmedov, A;Montecucco, F;Beer, JH;Lüscher, TF;Bongiovanni, D;Camici, GG;
    Thrombosis research
    Species: Transgenic Mouse
    Sample Types: Plasma, Tissue Homogenates
  2. Cognitive Alterations in Old Mice Are Associated with Intestinal Barrier Dysfunction and Induced Toll-like Receptor 2 and 4 Signaling in Different Brain Regions
    Authors: Brandt, A;Kromm, F;Hernández-Arriaga, A;Martínez Sánchez, I;Bozkir, HÖ;Staltner, R;Baumann, A;Camarinha-Silva, A;Heijtz, RD;Bergheim, I;
    Cells
    Species: Mouse
    Sample Types: Plasma
  3. Inhibition of PPAR? by BZ26, a GW9662 derivate, attenuated obesity-related breast cancer progression by inhibiting the reprogramming of mature adipocytes into to cancer associate adipocyte-like cells
    Authors: Li, L;Geng, J;Yu, W;Zhou, F;Zheng, Z;Fu, K;Kong, J;Feng, X;
    Frontiers in pharmacology
    Species: Mouse
    Sample Types: Serum
  4. Obesity promotes radioresistance through SERPINE1-mediated aggressiveness and DNA repair of triple-negative breast cancer
    Authors: YH Su, YZ Wu, DK Ann, JL Chen, CY Kuo
    Cell Death & Disease, 2023-01-21;14(1):53.
    Species: Mouse
    Sample Types: Serum
  5. Fibroblast growth factor 2 (FGF2) ameliorates the coagulation abnormalities in sepsis
    Authors: Y Sun, F Ye, D Li, H Yang, T Xu, X Zhong, Y Lu, H Zhou, J Pan
    Toxicology and Applied Pharmacology, 2023-01-06;460(0):116364.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Histone deacetylase 2 (HDAC2) attenuates lipopolysaccharide (LPS)-induced inflammation by regulating PAI-1 expression
    Authors: WF Fang, YM Chen, CY Lin, HL Huang, H Yeh, YT Chang, KT Huang, MC Lin
    J Inflamm (Lond), 2018-01-10;15(0):3.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Comparative systems pharmacology of HIF stabilization in the prevention of retinopathy of prematurity
    Authors: G Hoppe, S Yoon, B Gopalan, AR Savage, R Brown, K Case, A Vasanji, ER Chan, RB Silver, JE Sears
    Proc Natl Acad Sci USA, 2016-04-18;0(0):.
    Species: Mouse
    Sample Types: Serum

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