Mouse Serpin F2/ alpha ₂-Antiplasmin Biotinylated Antibody

Catalog #: BAF1239 Datasheet / COA / SDS

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BAF1239

All Serpin F2/alpha 2-Antiplasmin Antibodies

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Mouse Serpin F2/ alpha ₂-Antiplasmin Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin F2/ alpha 2‑Antiplasmin in Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Serpin F2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Serpin C1, rmSerpin D1, rhSerpin A1, and rhSerpin C1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin F2/ alpha 2‑Antiplasmin
Val28-Lys491
Accession # Q61247

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Serpin F2/ alpha ₂‑Antiplasmin (Catalog # 1239-PI)

Immunohistochemistry

5-15 µg/mL

Perfusion fixed frozen sections of mouse thymus

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin F2/alpha 2-Antiplasmin

Serpin F2 is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots (1, 2). In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin (3). Liver and kidney are major sites of Serpin F2 production and other tissues such as muscle, intestine, central nervous system, and placenta also express its mRNA at a moderate level (3). The tissue expression pattern of Serpin F2 indicates that it is a key regulator of plasmin-mediated proteolysis in these tissues. Mouse Serpin F2 is synthesized as a 491 amino acid precursor with a 27 amino acid signal peptide. The secreted protein has a short propeptide (residues 28-39) and a mature chain (residues 40-491). For human Serpin F2, the presence of the propeptide did not affect its ability to inhibit plasmin but reduced its cross-linking ability to fibrin (4).

References

Menoud, P-A. et al. (1996) J. Clin. Invest. 97:2478.
Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Potempa, J. et al. (1988) Science. 241:699.
Sumi, Y. et al. (1989) J. Biochem. 106:703.

Entrez Gene IDs

5345 (Human); 18816 (Mouse)

Alternate Names

AAPclade F (alpha-2 antiplasmin; alpha 2-Antiplasmin; Alpha-2-AP; Alpha-2-PI; Alpha-2-plasmin inhibitor; pigment epithelium derived factor), member 2; Serpin F2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 2