Mouse Serum Amyloid A Quantikine ELISA Kit

Catalog #: MSAA00
7 Citations Datasheet / COA / SDS

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MSAA00

Human Serum Amyloid A ELISA Standard Curve

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Citations (7)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse Serum Amyloid A Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Tissue Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)

Sensitivity

0.022 ng/mL

Assay Range

0.2 - 10 ng/mL (Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant mouse SAA.

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine® Mouse Serum Amyloid A Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse SAA in cell culture supernates, tissue culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse SAA1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse SAA. Results obtained using natural mouse SAA showed dose response curves that were parallel to the standard curves obtained using the Quantikine® mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse SAA.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Tissue Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.413	1.35	2.35	0.459	1.35	2.51
Standard Deviation	0.02	0.073	0.11	0.036	0.082	0.178
CV%	4.8	5.4	4.7	7.8	6.1	7.1

Recovery

The recovery of mouse SAA spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Culture Media (n=4)	93	84-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse SAA were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human Serum Amyloid A ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serum Amyloid A

The apolipoproteins are a structurally-unrelated group of proteins that have some association with the transport of lipids in blood. Apolipoproteins, plus phospholipids, cholesterol and triglycerides, form spherical particles with a lipid/hydrophobic center and a (apolipo)protein coat. The apolipoprotein coat promotes aqueous solubility and serves as a ligand for lipoprotein receptors. HDL may contain apolipoproteins A, C, D, E, J, L and M, while LDL contains apolipoproteins B and E.

ApoAI and ApoA2 are major protein components of serum high-density lipoprotein (HDL) and are produced by the liver and small intestine. They are involved in reverse cholesterol transport from tissues to the liver. Polymorphisms of ApoA2 are associated with disorders of cholesterol and fatty acid metabolism. Human ApoB (Apolipoprotein B-100) is a 550 kDa, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536 aa in length. It binds LDL to the ApoB/E receptor. ApoC activates lipoprotein lipase and may self-associate to form amyloid-type fibrils.

ApoE is a 34 kDa protein component of serum chylomicrons, VLDL, and HDL particles. It mediates the binding, uptake, and catabolism of these particles through interactions with the ApoE receptor and LDL receptors in the liver and brain. ApoE is important in fatty acid homeostasis and memory formation. Polymorphisms encode three variants (ApoE2, 3, 4) which are differentially related to the development of atherosclerosis and neurogenerative disorders, particularly Alzheimer's disease.

Serum amyloid A proteins (SAAs) are a family of homologous apolipoproteins of high density lipoprotein (HDL). They can be divided into two groups. The first group consists of the acute phase SAA1 and SAA2 that associate with HDL during inflammation and remodel the HDL particle by displacing apolipoprotein A1. The second group consists of constitutively expressed SAA4 and SAA5 that exist as minor apolipoproteins on HDL but make up more than 90% of the total SAA during homeostasis.

Alternate Names:

Serum Amyloid A

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse Serum Amyloid A Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Decreased liver B vitamin-related enzymes as a metabolic hallmark of cancer cachexia
Authors: Kojima, Y;Mishiro-Sato, E;Fujishita, T;Satoh, K;Kajino-Sakamoto, R;Oze, I;Nozawa, K;Narita, Y;Ogata, T;Matsuo, K;Muro, K;Taketo, MM;Soga, T;Aoki, M;
Nature communications
Species: Xenograft, Mouse, Transgenic Mouse
Sample Types: Plasma
Pre-Administration of PLX-R18 Cells Protects Mice from Radiation-Induced Hematopoietic Failure and Lethality
Authors: VP Kumar, S Biswas, GP Holmes-Ham, M Sheleg, S Stone, B Legesse, R Ofir, SP Ghosh
Genes, 2022-09-28;13(10):.
Species: Mouse
Sample Types: Serum
Renoprotective effects of ferric citrate in a mouse model of chronic kidney disease
Authors: MR Hanudel, B Czaya, S Wong, G Jung, K Chua, B Qiao, V Gabayan, T Ganz
Scientific Reports, 2022-04-23;12(1):6695.
Species: Mouse
Sample Types:
Serum amyloid A/anti-CCL20 induced the rebalance of Th17/regulatory T cells in SodA-induced sarcoidosis
Authors: K Meng, B Zhang, C Ma, Q Dai, X Gui, X Liu, Q Zhao, Q Gao, Y Wen, J Ding
International immunopharmacology, 2022-04-20;109(0):108784.
Species: Mouse
Sample Types: Serum
Upregulated endonuclease Regnase-1 suppresses osteoarthritis by forming a negative feedback loop of catabolic signaling in chondrocytes
Authors: JI Yang, JS Chun
Arthritis Research & Therapy, 2021-04-14;23(1):114.
Species: Mouse
Sample Types: Cell Culture Supernates
Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to PPARA agonism and fasting
Authors: CN Brocker, D Kim, T Melia, K Karri, TJ Velenosi, S Takahashi, D Aibara, JA Bonzo, M Levi, DJ Waxman, FJ Gonzalez
Nat Commun, 2020-11-17;11(1):5847.
Species: Mouse
Sample Types: Serum
Data on IL-10R neutralization-induced chronic colitis in Lipocalin 2 deficient mice on BALB/c background
Authors: V Singh, BS Yeoh, M Nilsen-Ham, T Berger, TW Mak, M Vijay-Kuma
Data Brief, 2017-03-08;11(0):588-592.
Species: Mouse
Sample Types: Serum