Mouse Siglec-3/CD33 Biotinylated Antibody

Catalog #: BAF2220 Datasheet / COA / SDS

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BAF2220

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Mouse Siglec-3/CD33 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Siglec‑3/CD33 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 is observed and less than 5% cross-reactivity with recombinant mouse (rm) Siglec-F, rmSiglec-2, rhSiglec-3, rhSiglec-5 and rhSiglec-10 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Siglec‑3/CD33
Gln17-Val244
Accession # Q63994

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Siglec‑3/CD33

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-3/CD33

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) cell-surface lectins that recognize sialic acid within specific glycan structures. Mouse Siglec-3, also known as CD33 and GP67, is expressed primarily on myeloid precursors in the bone marrow and on granulocytes in peripheral blood. Two isoforms have been reported for mouse Siglec-3 that display distinct cytoplasmic domains resulting from differential mRNA splicing.

Long Name

Sialic Acid Binding Ig-like Lectin 3

Entrez Gene IDs

945 (Human); 12489 (Mouse); 102117580 (Cynomolgus Monkey)

Alternate Names

CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33; FLJ00391; gp67; myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec3; Siglec-3; SIGLEC3gp67