Mouse TIM-5 Antibody

Catalog # Availability Size / Price Qty
MAB3374
MAB3374-SP
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Mouse TIM-5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TIM-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse TIM-1, -2, -3, -4, or -6 is observed.
Source
Monoclonal Rat IgG1 Clone # 339319
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIM‑5
Phe22-Gly161
Accession # NP_001156829
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TIM‑5 under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-5

Mouse TIM-5 (T cell immunoglobulin mucin-5; also hepatitis A virus cellular receptor 1-like) is presumably a member of the TIM family of immune regulatory proteins. Based on other TIM molecules, it will be a type I transmembrane protein with one V-type Ig-like domain and a Ser/Thr-rich mucin region. Mouse TIM-5 has 47% aa identity to mouse TIM-2.

Long Name
T Cell Immunoglobulin Mucin-5
Entrez Gene IDs
210535 (Mouse)
Alternate Names
TIM5; TIM-5

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