Mouse TIMP-1 Antibody

Name: Mouse TIMP-1 Antibody
Brand: R&D Systems
SKU: AF980
Rating: 4.5 (2 reviews)

Catalog #: AF980
25 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF980
AF980-SP

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Citations (25)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TIMP-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat TIMP-1 is observed, less than 5% cross-reactivity with recombinant human (rh) TIMP-1 is observed, and less than 1% cross-reactivity with rhTIMP-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIMP-1
Cys25-Arg205
Accession # P12032

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TIMP‑1 Western Blot Standard (Catalog # WBC022)

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse TIMP‑1 (Catalog # 980‑MT), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Mouse TIMP-1 (0.07 µg/mL, Catalog # 980-MT) inhibition of Recombinant Mouse/Rat MMP‑2 (0.2 µg/mL, Catalog # 924-MP) cleavage of the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH₂ (10 µM, Catalog # ES001). The Neutralization Dose (ND₅₀) is typically 1.3 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

TIMP-1 in Mouse Ovary. TIMP-1 was detected in perfusion fixed frozen sections of mouse ovary using Goat Anti-Mouse TIMP-1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # AF980) at 15 µg/mL overnight at 4 °C.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of homologous proteins that regulate the activity of matrix metalloproteinases (MMPs) (1, 2). There are four known members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 that have been found to exhibit multiple functions, including inhibition of active MMPs, pro‑MMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Structurally, TIMPs have two domains, an N‑terminal domain and a C-terminal domain. Each domain consists of three disulfide-bonded loops. TIMP-1 is a glycoprotein produced by a wide range of cell types. Through its N-terminal domain, TIMP-1 inhibits active MMPs by forming a non-covalent binary complex with the MMP active site. The C-terminal domain of TIMP‑1 interacts with the C-terminal domain of pro‑MMP‑9, which may play a role in regulating pro‑MMP‑9 activation.

References

Murphy, G. and F. Willenbrock (1995) Methods Enzymol. 248:496.
Brew, K. et al. (2000) Biochim. Biophys. Acta 1477:267.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Entrez Gene IDs

7076 (Human); 21857 (Mouse); 116510 (Rat)

Alternate Names

CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse TIMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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Orthologous proteins of experimental de- and remyelination are differentially regulated in the CSF proteome of multiple sclerosis subtypes
Authors: Nellie A. Martin, Arkadiusz Nawrocki, Viktor Molnar, Maria L. Elkjaer, Eva K. Thygesen, Miklos Palkovits et al.
PLOS ONE
Ym1+ macrophages orchestrate fibrosis, lesion growth and progression during development of murine pancreatic cancer
Authors: Fleming Martinez A, DOppler H, Bastea L et al.
iScience
Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma Cells
Authors: Varga, AJ;Nemeth, IB;Kemeny, L;Varga, J;Tiszlavicz, L;Kumar, D;Dodd, S;Simpson, AWM;Buknicz, T;Beynon, R;Simpson, D;Krenacs, T;Dockray, GJ;Varro, A;
International journal of molecular sciences
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
Regorafenib prevents the development of emphysema in a murine elastase model
Authors: Kwangseok Oh, Gun-Wu Lee, Han-Byeol Kim, Jin-Hee Park, Eun-Young Shin, Eung-Gook Kim
BMB Reports
Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor indicates a cell-specific response that promotes CD44 activation
Authors: D Piekna-Prz, D Na, J Zhang, C Baker, JM Ashton, PM White
Frontiers in Cellular Neuroscience, 2023-01-06;16(0):1096872.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-gamma
Authors: Catherine S. Coleman, Bruce A. Stanley, Charles H. Lang
Inflammation
Matrix metalloproteinases and tissue inhibitors of metalloproteinases in murine beta -coronavirus-induced neuroinflammation
Authors: Sourodip Sengupta, Sankar Addya, Diptomit Biswas, Paromita Banerjee, Jayasri Das Sarma
Virology
Myeloid-Derived TSP1 (Thrombospondin-1) Contributes to Abdominal Aortic Aneurysm Through Suppressing Tissue Inhibitor of Metalloproteinases-1
Authors: Huan Yang, Ting Zhou, Christine M. Sorenson, Nader Sheibani, Bo Liu
Arteriosclerosis, Thrombosis, and Vascular Biology
CD73+ extracellular vesicles inhibit angiogenesis through adenosine A2B receptor signalling
Authors: Roberta Angioni, Cristina Liboni, Stephanie Herkenne, Ricardo Sánchez‐Rodríguez, Giulia Borile, Elisabetta Marcuzzi et al.
Journal of Extracellular Vesicles
In Vivo Activation and Pro-Fibrotic Function of NF-kappaB in Fibroblastic Cells During Pulmonary Inflammation and Fibrosis Induced by Carbon Nanotubes
Authors: J Dong, Q Ma
Front Pharmacol, 2019-10-02;10(0):1140.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis
Authors: N Giebeler, A Schönefu beta, J Landsberg, T Tüting, C Mauch, P Zigrino
Oncogene, 2017-05-29;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Mouse mesenchymal stem cells inhibit high endothelial cell activation and lymphocyte homing to lymph nodes by releasing TIMP-1
Authors: L Zanotti, R Angioni, B Calì, C Soldani, C Ploia, F Moalli et al.
Leukemia

Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice
Authors: MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré
J. Biol. Chem., 2015-12-09;291(15):8070-89.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities.
Authors: Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, Hachet C, Ferracci G, David M, Martiny L, Charpentier E, Khrestchatisky M, Rivera S, Dedieu S, Emonard H
PLoS ONE, 2014-07-30;9(7):e103839.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot

TAp73 is essential for germ cell adhesion and maturation in testis.
Authors: Holembowski L, Kramer D, Riedel D, Sordella R, Nemajerova A, Dobbelstein M, Moll U
J Cell Biol, 2014-03-24;204(7):1173-90.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Tissue inhibitor of matrix metalloproteinases-1 loaded poly(lactic-co-glycolic acid) nanoparticles for delivery across the blood–brain barrier
Authors: Mayank Chaturvedi, Yves Molino, Sreedhar Sreedhar, Michel Khrestchatisky, Leszek Kaczmarek
International Journal of Nanomedicine

Angiogenic capacity of M1- and M2-polarized macrophages is determined by the levels of TIMP-1 complexed with their secreted proMMP-9
Authors: Ewa Zajac, Bernhard Schweighofer, Tatyana A. Kupriyanova, Anna Juncker-Jensen, Petra Minder, James P. Quigley et al.
Blood

Regulation of PDGFC signalling and extracellular matrix composition by FREM1 in mice
Authors: Fenny Wiradjaja, Denny L. Cottle, Lynelle Jones, Ian Smyth
Disease Models & Mechanisms

The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration.
Authors: Kim Y, Remacle AG, Chernov AV
PLoS ONE, 2012-03-16;7(3):e33664.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes.
Authors: Sbai O, Ould-Yahoui A, Ferhat L, Gueye Y, Bernard A, Charrat E, Mehanna A, Risso JJ, Chauvin JP, Fenouillet E, Rivera S, Khrestchatisky M
Glia, 2010-02-01;58(3):344-66.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Electron Microscopy, ICC, Western Blot

Amelioration of coxsackievirus B3-mediated myocarditis by inhibition of tissue inhibitors of matrix metalloproteinase-1.
Authors: Crocker SJ, Frausto RF, Whitmire JK, Benning N, Milner R, Whitton JL
Am. J. Pathol., 2007-11-30;171(6):1762-73.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization

Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice.
Authors: Kim KH, Burkhart K, Chen P, Frevert CW, Randolph-Habecker J, Hackman RC, Soloway PD, Madtes DK
Am. J. Respir. Cell Mol. Biol., 2005-06-09;33(3):271-9.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis.
Authors: Chen KM, Lee HH, Chou HL, Liu JY, Tsai BC, Lai SC
Int J Exp Pathol, 2005-04-01;86(2):81-9.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot

TIMP1 expression underlies sex disparity in liver metastasis and survival in pancreatic cancer
Authors: Chris D. Hermann, Benjamin Schoeps, Celina Eckfeld, Enkhtsetseg Munkhbaatar, Lukas Kniep, Olga Prokopchuk et al.
Journal of Experimental Medicine

Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination
Authors: Abraham V, Cao G, Parambath A et al.
Int J Onc