TROP-2 is alternatively known as tumor-associated calcium signal transducer 2 and lymphocyte antigen 97.
Mouse TROP‑2 Biotinylated Antibody
R&D Systems | Catalog # BAF1122
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Western Blot, Flow Cytometry, Immunoprecipitation
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TROP-2
Ser82-Thr268
Accession # Q8BGV3
Ser82-Thr268
Accession # Q8BGV3
Specificity
Detects mouse TROP-2 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) TROP-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse TROP‑2 Biotinylated Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse ovary
Sample: Perfusion fixed frozen sections of mouse ovary
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse TROP‑2
Sample: Recombinant Mouse TROP‑2
Reviewed Applications
Read 1 review rated 5 using BAF1122 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TROP-2
Long Name
Tumor-associated Calcium Signal Transducer 2
Alternate Names
GA733-1, gp50, T16, TACSTD2, TROP2
Gene Symbol
TACSTD2
UniProt
Additional TROP-2 Products
Product Documents for Mouse TROP‑2 Biotinylated Antibody
Product Specific Notices for Mouse TROP‑2 Biotinylated Antibody
For research use only
Related Research Areas
Citations for Mouse TROP‑2 Biotinylated Antibody
Customer Reviews for Mouse TROP‑2 Biotinylated Antibody (1)
5 out of 5
1 Customer Rating
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Application: Flow CytometrySample Tested: 4T1 mouse breast cancer cell lineSpecies: MouseVerified Customer | Posted 11/01/2021Detection of mouse TROP2 on the 4T1 breast cancer mouse cell line. 4T1 cells were treated with the Mouse TROP-2 Biotinylated antibody (catalog # BAF1122) (BLUE) or Normal Goat IgG biotinylated control antibody (catalog # BAF108) (RED), followed by streptavidin conjugated to APC.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars