Mouse Tryptase beta-1/MCP-7/Mcpt7 Biotinylated Antibody

Catalog #: BAF1937
2 Citations Datasheet / COA / SDS

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BAF1937

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Tryptase beta-1/MCP-7/Mcpt7 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Tryptase beta ‑1/MCP‑7/Mcpt7 in Western blots. In Western blots, less than 1% cross‑reactivity with recombinant mouse MCPT1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Tryptase beta ‑1/MCP‑7/Mcpt7
Ile29-Phe273
Accession # Q02844

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Tryptase beta ‑1/MCP‑7/Mcpt7 (Catalog # 1937-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase beta-1/MCP-7

Tryptase beta -1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid sequence for mouse Tryptase beta -1 consists of a signal peptide (residues 1‑18), a pro region (residues 19‑28) and the mature chain (residue 29‑273). The mature chain was expressed and purified.

References

McNeil, H.P. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11174.
Matsumoto, R et al. (1995) J. Biol. Chem. 270:19524.
Huang, C. et al. (1997) J. Biol. Chem. 272:31885.

Long Name

Mast Cell Protease 7

Entrez Gene IDs

100503895 (Mouse)

Alternate Names

Mast Cell Tryptase; Mcp7; MCP-7; Mcpt7; TPSB1; TRYB1; Tryptase beta1; Tryptase beta-1

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse Tryptase beta-1/MCP-7/Mcpt7 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Suppression of IgE-mediated anaphylaxis and food allergy with monovalent anti-Fc epsilon RI alpha monoclonal antibodies
Authors: Marat V. Khodoun, Suzanne C. Morris, Wen-Hai Shao, Crystal Potter, Elizabeth Angerman, Artem Kiselev et al.
Journal of Allergy and Clinical Immunology
Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice
Authors: M Cecilia Berin
Plant Biotechnol. J., 2016-01-23;14(7):1541-50.
Species: Mouse
Sample Types: Serum
Applications: ELISA Development (Detection)