Mouse VCAM-1/CD106 Antibody Summary
Phe25-Glu698
Accession # P29533
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Mouse VCAM‑1/CD106 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for VCAM-1/CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VCAM‑1/CD106 in Mouse Embryo. VCAM-1/CD106 was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of VCAM‑1/CD106 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643, filled histogram) or control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107).
Detection of Mouse VCAM‑1/CD106 by Simple WesternTM. Simple Western lane view shows lysates of C2C12 mouse myoblast cell line and 3T3-L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM-1/CD106 at approximately 116 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse VCAM-1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody ( HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of VCAM‑1/CD106 in bEND.3 cells by Flow Cytometry. bEND.3 cells were stained with Goat Anti-Mouse VCAM‑1/CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF643, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse VCAM-1/CD106 by Western Blot Markers of endothelial dysfunction in aorta.Expression of endoglin (A, B), eNOS (C, D), ICAM-1 (E, F) and VCAM-1 (G, H) in total protein extracts from mice aorta. Equal loading of samples was confirmed by immunodetection of GADPH (A, B). Top: densitometric analysis (control = 100%). Bottom: representative immunoblots. Data are shown as mean ± S.E.M. Mann-Whitney test, unpaired t-test. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0119665), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse VCAM-1/CD106 by Western Blot Markers of endothelial dysfunction in aorta.Expression of endoglin (A, B), eNOS (C, D), ICAM-1 (E, F) and VCAM-1 (G, H) in total protein extracts from mice aorta. Equal loading of samples was confirmed by immunodetection of GADPH (A, B). Top: densitometric analysis (control = 100%). Bottom: representative immunoblots. Data are shown as mean ± S.E.M. Mann-Whitney test, unpaired t-test. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0119665), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Mouse VCAM-1/CD106 Antibody by Western Blot Markers of endothelial dysfunction in aorta.Expression of endoglin (A, B), eNOS (C, D), ICAM-1 (E, F) and VCAM-1 (G, H) in total protein extracts from mice aorta. Equal loading of samples was confirmed by immunodetection of GADPH (A, B). Top: densitometric analysis (control = 100%). Bottom: representative immunoblots. Data are shown as mean ± S.E.M. Mann-Whitney test, unpaired t-test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25768936), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VCAM-1/CD106
VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.
During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.
ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.
Product Datasheets
Citations for Mouse VCAM-1/CD106 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Insulin decreases atherosclerosis by inducing endothelin receptor B expression.
Authors: Park K, Mima A, Li Q et al.
JCI Insight.
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Nicotinamide mononucleotide attenuates brain injury after intracerebral hemorrhage by activating Nrf2/HO-1 signaling pathway
Authors: CC Wei, YY Kong, GQ Li, YF Guan, P Wang, CY Miao
Sci Rep, 2017-04-06;7(1):717.
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Multi-omics profiling of mouse polycystic kidney disease progression at a single cell resolution
Authors: Muto, Y;Yoshimura, Y;Wu, H;Chang-Panesso, M;Ledru, N;Woodward, OM;Outeda, P;Cheng, T;Mahjoub, MR;Watnick, TJ;Humphreys, BD;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
Kupffer Cell Inactivation Alters Endothelial Cell Adhesion Molecules in Cecal Ligation and Puncture-Induced Sepsis
Authors: Manandhar, S;Gaddam, RR;Chambers, S;Bhatia, M;
Biomolecules
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Pharmacological Inhibition and Genetic Deletion of Cystathionine Gamma-Lyase in Mice Protects against Organ Injury in Sepsis: A Key Role of Adhesion Molecules on Endothelial Cells
Authors: Manandhar, S;Chambers, S;Miller, A;Ishii, I;Bhatia, M;
International journal of molecular sciences
Species: Transgenic Mouse, Mouse
Sample Types: Whole Tissue
Applications: IHC -
Macrophage-to-endothelial cell crosstalk by the cholesterol metabolite 27HC promotes atherosclerosis in male mice
Authors: Yu, L;Xu, L;Chu, H;Peng, J;Sacharidou, A;Hsieh, HH;Weinstock, A;Khan, S;Ma, L;Durán, JGB;McDonald, J;Nelson, ER;Park, S;McDonnell, DP;Moore, KJ;Huang, LJ;Fisher, EA;Mineo, C;Huang, L;Shaul, PW;
Nature communications
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Reprogramming tumour-associated macrophages to outcompete cancer cells
Authors: Zhang, X;Li, S;Malik, I;Do, MH;Ji, L;Chou, C;Shi, W;Capistrano, KJ;Zhang, J;Hsu, TW;Nixon, BG;Xu, K;Wang, X;Ballabio, A;Schmidt, LS;Linehan, WM;Li, MO;
Nature
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Aedes aegypti salivary gland extract alleviates acute itching by blocking TRPA1 channels
Authors: Anderson R. A. Cerqueira, Leandro Rodrigues, Silvia Abigail Coavoy-Sánchez, Simone A. Teixeira, Karla B. Feitosa, Erika Y. Taniguchi et al.
Frontiers in Physiology
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Production of Dermatophagoides farinae Having Low Bacterial Content Using Ampicillin
Authors: Kim, JY;Yi, MH;Kim, M;Choi, JH;Lee, S;Yong, TS;
Journal of immunology research
Species: Mouse
Sample Types: Whole Tissue
Applications: Immunohistochemistry -
STAT3/NF‑ kappa B decoy oligodeoxynucleotides inhibit atherosclerosis through regulation of the STAT/NF‑ kappa B signaling pathway in a mouse model of atherosclerosis
Authors: Hyun-Jin An, Mi-Gyeong Gwon, Hyemin Gu, Seongjae Bae, Jaechan Leem, Jin Bae Lee et al.
International Journal of Molecular Medicine
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Development of activated endothelial targeted high-density lipoprotein nanoparticles
Authors: Minzhi Yu, Kristen Hong, Reheman Adili, Ling Mei, Lisha Liu, Hongliang He et al.
Frontiers in Pharmacology
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Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-gamma
Authors: Catherine S. Coleman, Bruce A. Stanley, Charles H. Lang
Inflammation
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Imbalanced Activation of Wnt-/ beta -Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation
Authors: Philipp-Sebastian Koch, Kajetan Sandorski, Joschka Heil, Christian D. Schmid, Sina W. Kürschner, Johannes Hoffmann et al.
Frontiers in Physiology
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HO‑1 knockdown upregulates the expression of VCAM‑1 to induce neutrophil recruitment during renal ischemia‑reperfusion injury
Authors: Yecheng He, Huadong Li, Juan Yao, Hua Zhong, Yanbin Kuang, Xin Li et al.
International Journal of Molecular Medicine
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Endothelial NADPH oxidase 4 protects against angiotensin II‐induced cardiac fibrosis and inflammation
Authors: Minshu Wang, Colin E. Murdoch, Alison C. Brewer, Aleksandar Ivetic, Paul Evans, Ajay M. Shah et al.
ESC Heart Failure
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The protective role of proton-sensing TDAG8 in the brain injury in a mouse ischemia reperfusion model
Authors: K Sato, A Tobo, C Mogi, M Tobo, N Yamane, M Tosaka, H Tomura, DS Im, F Okajima
Sci Rep, 2020-10-14;10(1):17193.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm
Authors: SJ Jeong, MJ Cho, NY Ko, S Kim, IH Jung, JK Min, SH Lee, JG Park, GT Oh
Exp. Mol. Med., 2020-09-14;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Senescence-Induced Vascular Remodeling Creates Therapeutic Vulnerabilities in Pancreas Cancer
Authors: Marcus Ruscetti, John P. Morris, Riccardo Mezzadra, James Russell, Josef Leibold, Paul B. Romesser et al.
Cell
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Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis
Authors: S Colijn, V Muthukumar, J Xie, S Gao, CT Griffin
Dis Model Mech, 2020-01-24;13(1):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Apoptosis signal-regulating kinase 1 activation by Nox1-derived oxidants is required for TNF alpha receptor endocytosis
Authors: Hyehun Choi, Ryan J. Stark, Benjamin S. Raja, Anna Dikalova, Fred S. Lamb
American Journal of Physiology-Heart and Circulatory Physiology
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Cell-Type-Specific Interleukin 1 Receptor 1 Signaling in the Brain Regulates Distinct Neuroimmune Activities
Authors: Xiaoyu Liu, Daniel P. Nemeth, Daniel B. McKim, Ling Zhu, Damon J. DiSabato, Olimpia Berdysz et al.
Immunity
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Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells
Authors: P Wohlrab, L Soto-Gonza, T Benesch, MP Winter, IM Lang, K Markstalle, V Tretter, KU Klein
Front Physiol, 2018-12-06;9(0):1754.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus
Authors: FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser
Elife, 2018-09-06;7(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Neutrophil infiltration to the brain is platelet-dependent, and is reversed by blockade of platelet GPIb?
Authors: JA Giles, AD Greenhalgh, A Denes, B Nieswandt, G Coutts, BW McColl, SM Allan
Immunology, 2018-02-08;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Suberanilohydroxamic Acid as a Pharmacological Kruppel-Like Factor 2 Activator That Represses Vascular Inflammation and Atherosclerosis
Authors: Y Xu, S Xu, P Liu, M Koroleva, S Zhang, S Si, ZG Jin
J Am Heart Assoc, 2017-11-30;6(12):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Thioredoxin-interacting protein promotes high-glucose-induced macrovascular endothelial dysfunction
Authors: Xiaoyu Li, Karen L. Kover, Daniel P. Heruth, Dara J. Watkins, Yanchun Guo, Wayne V. Moore et al.
Biochemical and Biophysical Research Communications
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Endothelium-derived extracellular vesicles promote splenic monocyte mobilization in myocardial infarction
Authors: N Akbar, JE Digby, TJ Cahill, AN Tavare, AL Corbin, S Saluja, S Dawkins, L Edgar, N Rawlings, K Ziberna, E McNeill, E Johnson, AA Aljabali, RA Dragovic, M Rohling, TG Belgard, IA Udalova, DR Greaves, KM Channon, PR Riley, DC Anthony, RP Choudhury
JCI Insight, 2017-09-07;2(17):.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Cell Selection -
Loss of Ptpn11 (Shp2) drives satellite cells into quiescence
Authors: J Griger, R Schneider, I Lahmann, V Schöwel, C Keller, S Spuler, M Nazaré, C Birchmeier
Elife, 2017-05-02;6(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Ventilator-induced lung injury increases expression of endothelial inflammatory mediators in the kidney
Authors: Mark Hepokoski, Joshua A. Englert, Rebecca M. Baron, Laura E. Crotty-Alexander, Mark M. Fuster, Jeremy R. Beitler et al.
American Journal of Physiology-Renal Physiology
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Numb regulates somatic cell lineage commitment during early gonadogenesis in mice
Authors: YT Lin, L Barske, T DeFalco, B Capel
Development, 2017-03-30;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
GPR4 deficiency alleviates intestinal inflammation in a mouse model of acute experimental colitis
Authors: Edward J. Sanderlin, Nancy R. Leffler, Kvin Lertpiriyapong, Qi Cai, Heng Hong, Vasudevan Bakthavatchalu et al.
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease
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LRRC8A channels support TNF alpha -induced superoxide production by Nox1 which is required for receptor endocytosis
Authors: Hyehun Choi, Nicholas Ettinger, Jeffrey Rohrbough, Anna Dikalova, Hong N. Nguyen, Fred S. Lamb
Free Radical Biology and Medicine
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Nonsurgical periodontal treatment reduced aortic inflammation in ApoE(-/-) mice with periodontitis
Authors: Di Cui, Houxuan Li, Lang Lei, Changxing Chen, Fuhua Yan
SpringerPlus
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Endothelial Msx1 transduces hemodynamic changes into an arteriogenic remodeling response
Authors: Ine Vandersmissen, Sander Craps, Maarten Depypere, Giulia Coppiello, Nick van Gastel, Frederik Maes et al.
Journal of Cell Biology
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Social defeat promotes a reactive endothelium in a brain region-dependent manner with increased expression of key adhesion molecules, selectins and chemokines associated with the recruitment of myeloid cells to the brain
Authors: Caroline M. Sawicki, Daniel B. McKim, Eric S. Wohleb, Brant L. Jarrett, Brenda F. Reader, Diana M. Norden et al.
Neuroscience
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Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals.
Authors: Cedervall J, Zhang Y, Huang H, Zhang L, Femel J, Dimberg A, Olsson A
Cancer Res, 2015-06-12;75(13):2653-62.
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Platelet-driven leukotriene C4-mediated airway inflammation in mice is aspirin-sensitive and depends on T prostanoid receptors.
Authors: Liu T, Garofalo D, Feng C, Lai J, Katz H, Laidlaw T, Boyce J
J Immunol, 2015-04-22;194(11):5061-8.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
High soluble endoglin levels do not induce endothelial dysfunction in mouse aorta.
Authors: Nemeckova I, Serwadczak A, Oujo B, Jezkova K, Rathouska J, Fikrova P, Varejckova M, Bernabeu C, Lopez-Novoa J, Chlopicki S, Nachtigal P
PLoS ONE, 2015-03-13;10(3):e0119665.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Control of the T follicular helper-germinal center B-cell axis by CD8(+) regulatory T cells limits atherosclerosis and tertiary lymphoid organ development.
Authors: Clement M, Guedj K, Andreata F, Morvan M, Bey L, Khallou-Laschet J, Gaston A, Delbosc S, Alsac J, Bruneval P, Deschildre C, Le Borgne M, Castier Y, Kim H, Cantor H, Michel J, Caligiuri G, Nicoletti A
Circulation, 2014-12-31;131(6):560-70.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Icam-1 targeted nanogels loaded with dexamethasone alleviate pulmonary inflammation.
Authors: Ferrer M, Shuvaev V, Zern B, Composto R, Muzykantov V, Eckmann D
PLoS ONE, 2014-07-14;9(7):e102329.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
CD44-deficiency attenuates the immunologic responses to LPS and delays the onset of endotoxic shock-induced renal inflammation and dysfunction.
Authors: Rampanelli, Elena, Dessing, Mark C, Claessen, Nike, Teske, Gwendoli, Joosten, Sander P, Pals, Steven T, Leemans, Jaklien, Florquin, Sandrine
PLoS ONE, 2013-12-23;8(12):e84479.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Effect of Rosiglitazone on Liver Structure and Function in Genetically Diabetic Akita Mice
Authors: Bianca Hemmeryckx, Marijke Gaekens, David J. Gallacher, Hua Rong Lu, Henri Roger Lijnen
Basic & Clinical Pharmacology & Toxicology
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Micro RNA ‐146 represses endothelial activation by inhibiting pro‐inflammatory pathways
Authors: Henry S. Cheng, Nirojini Sivachandran, Andrew Lau, Emilie Boudreau, Jimmy L. Zhao, David Baltimore et al.
EMBO Molecular Medicine
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The Transcription Factors Tbx18 and Wt1 Control the Epicardial Epithelial-Mesenchymal Transition through Bi-Directional Regulation of Slug in Murine Primary Epicardial Cells
Authors: Makiko Takeichi, Keisuke Nimura, Masaki Mori, Hironori Nakagami, Yasufumi Kaneda
PLoS ONE
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Age-associated pro-inflammatory adaptations of the mouse thoracic aorta
Authors: Bianca Hemmeryckx, Marc F. Hoylaerts, Eveline Deloose, Cor E. Van Hove, Paul Fransen, Hidde Bult et al.
Thrombosis and Haemostasis
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Targeting improves MSC treatment of inflammatory bowel disease.
Authors: Ko IK, Kim BG, Awadallah A
Mol. Ther., 2010-04-13;18(7):1365-72.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes.
Authors: Katagiri K, Katakai T, Ebisuno Y, Ueda Y, Okada T, Kinashi T
EMBO J., 2009-04-02;28(9):1319-31.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Dietary alpha-lipoic acid supplementation inhibits atherosclerotic lesion development in apolipoprotein E-deficient and apolipoprotein E/low-density lipoprotein receptor-deficient mice.
Authors: Zhang WJ, Bird KE, McMillen TS, LeBoeuf RC, Hagen TM, Frei B
Circulation, 2007-12-24;117(3):421-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Mice lacking NKCC1 are protected from development of bacteremia and hypothermic sepsis secondary to bacterial pneumonia.
Authors: Nguyen M, Pace AJ, Koller BH
J. Exp. Med., 2007-05-21;204(6):1383-93.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
High pressure promotes monocyte adhesion to the vascular wall.
Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
Circ. Res., 2007-03-29;100(8):1226-33.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
Blood, 2007-01-16;109(9):3706-12.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Re-purposing the pro-senescence properties of doxorubicin to introduce immunotherapy in breast cancer brain metastasis
Authors: Rebeca Uceda-Castro, Andreia S. Margarido, Lesley Cornet, Serena Vegna, Kerstin Hahn, Ji-Ying Song et al.
Cell Reports Medicine
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paraffin-embedded tissue
antigen retrieval in citrate buffer pH 6
concentrations tested: 1:50-1:300 all worked