Mouse VCAM-1/CD106 DuoSet ELISA

Catalog #: DY643
11 Citations Datasheet / COA / SDS

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DY643

Ancillary Products Available

DY008B: DuoSet ELISA Ancillary Reagent Kit 2

Mouse VCAM-1 / CD106 ELISA Standard Curve

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All VCAM-1/CD106 ELISAs

Product Details

Procedure

Citations (11)

Supplemental Products

Reviews

Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Mouse VCAM-1/CD106 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Assay Length

4 hours 40 minutes (after plate preparation)

Sample Volume Required

100 µL

Assay Range

125.0 - 8,000 pg/mL

Sufficient Materials

For fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse VCAM-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween^® 20 in PBS, pH 7.2-7.4

Block Buffer: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Reagent Diluent: 0.1% BSA, 0.05% Tween 20 in Tris-buffered Saline (20 mM Trizma base, 150 mM NaCI) pH 7.2-7.4, 0.2 μm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data

N/A

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Mouse VCAM-1 / CD106 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VCAM-1/CD106

VCAM-1 (Vascular Cell Adhesion Molecule-1; CD106) is a transmembrane molecule that mediates the adhesion of immune cells to the vascular endothelium during inflammation. It binds to several integrins including alpha4/beta1 (VLA-4), alpha4/beta7, alpha9/beta1, and alphaD/beta2. It is expressed constitutively in the bone marrow where it regulates T cell, B cell, and hematopoietic progenitor cell migration. A soluble form VCAM-1 can promote monocyte chemotaxis.

Long Name:

Vascular Cell Adhesion Molecule 1

Entrez Gene IDs:

7412 (Human); 22329 (Mouse)

Alternate Names:

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
Block plates by adding 300 μL of Block Buffer to each well. Incubate at room temperature for a minimum of 1 hour.
Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
Repeat the aspiration/wash as in step 2 of Plate Preparation.
Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
Repeat the aspiration/wash as in step 2 of Plate Preparation.
Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
Repeat the aspiration/wash as in step 2.
Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse VCAM-1/CD106 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Kupffer Cell Inactivation Alters Endothelial Cell Adhesion Molecules in Cecal Ligation and Puncture-Induced Sepsis
Authors: Manandhar, S;Gaddam, RR;Chambers, S;Bhatia, M;
Biomolecules
Species: Mouse
Sample Types: Tissue Homogenates
Pharmacological Inhibition and Genetic Deletion of Cystathionine Gamma-Lyase in Mice Protects against Organ Injury in Sepsis: A Key Role of Adhesion Molecules on Endothelial Cells
Authors: Manandhar, S;Chambers, S;Miller, A;Ishii, I;Bhatia, M;
International journal of molecular sciences
Species: Mouse, Transgenic Mouse
Sample Types: Tissue Homogenates
Therapeutic Intervention with Anti-Complement Component 5 Antibody Does Not Reduce NASH but Does Attenuate Atherosclerosis and MIF Concentrations in Ldlr-/-.Leiden Mice
Authors: F Seidel, R Kleemann, W van Duyven, N van Trigt, N Keijzer, S van der Ko, C van Kooten, L Verschuren, A Menke, AJ Kiliaan, J Winter, TR Hughes, BP Morgan, F Baas, K Fluiter, MC Morrison
International Journal of Molecular Sciences, 2022-09-14;23(18):.
Species: Mouse
Sample Types: Plasma
Agomelatine Attenuates Isoflurane-Induced Inflammation and Damage in Brain Endothelial Cells
Authors: F Cheng, H Chang, F Yan, A Yang, J Liu, Y Liu
Drug Design, Development and Therapy, 2020-12-18;14(0):5589-5598.
Species: Mouse
Sample Types: Cell Culture Supernates
Soluble VCAM-1 promotes gemcitabine resistance via macrophage infiltration and predicts therapeutic response in pancreatic cancer
Authors: R Takahashi, H Ijichi, M Sano, K Miyabayash, D Mohri, J Kim, G Kimura, T Nakatsuka, H Fujiwara, K Yamamoto, Y Kudo, Y Tanaka, K Tateishi, Y Nakai, Y Morishita, K Soma, N Takeda, HL Moses, H Isayama, K Koike
Scientific Reports, 2020-12-03;10(1):21194.
Species: Mouse
Sample Types: Cell Culture Supernates
The Impact of Focused Ultrasound in Two Tumor Models: Temporal Alterations in the Natural History on Tumor Microenvironment and Immune Cell Response
Authors: G Cohen, P Chandran, RM Lorsung, LE Tomlinson, M Sundby, SR Burks, JA Frank
Cancers (Basel), 2020-02-04;12(2):.
Species: Mouse
Sample Types: Cell Culture Supernates
Combination of a high-fat diet with sweetened condensed milk exacerbates inflammation and insulin resistance induced by each separately in mice
Authors: LN Masi, AR Martins, AR Crisma, CL do Amaral, MR Davanso, TDA Serdan, RDC da Cunha d, MM Cruz, MIC Alonso-Val, RP Torres, J Mancini-Fi, JNB Pereira, MM da Silva R, EA Liberti, SM Hirabara, R Curi
Sci Rep, 2017-06-21;7(1):3937.
Species: Mouse
Sample Types: Tissue Homogenates
Imbalance of the Vanin-1 Pathway in Systemic Sclerosis
J Immunol, 2016-09-19;0(0):.
Species: Mouse
Sample Types: Serum
Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
Authors: Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
J. Immunol., 2007-11-15;179(10):7166-75.
Species: Human, Mouse
Sample Types: Urine
Neurokinin-1 receptor antagonist treatment protects mice against lung injury in polymicrobial sepsis.
Authors: Hegde A, Zhang H, Moochhala SM, Bhatia M
J. Leukoc. Biol., 2007-06-12;82(3):678-85.
Species: Mouse
Sample Types: Plasma

Excreted urinary mediators in an animal model of experimental immune nephritis with potential pathogenic significance.
Authors: Wu T, Xie C, Bhaskarabhatla M, Yan M, Leone A, Chen SS, Zhou XJ, Putterman C, Mohan C
Arthritis Rheum., 2007-03-01;56(3):949-59.
Species: Mouse
Sample Types: Urine