Mouse VEGFR1/Flt-1 APC-conjugated Antibody

Catalog #: FAB4711A
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB4711A

Detection of VEGF R1/Flt‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.

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All VEGFR1/Flt-1 Antibodies

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Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGFR1/Flt-1 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse VEGF R1/Flt‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human VEGF R1, recombinant mouse (rm) VEGF R2, or rmVEGF R3 is observed.

Source

Monoclonal Rat IgG_2B Clone # 141522

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VEGF R1/Flt‑1
Ser27-Glu759
Accession # P35969

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of VEGF R1/Flt‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse VEGF R1/Flt-1 APC-conjugated Monoclonal Antibody (Catalog # FAB4711A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: VEGFR1/Flt-1

VEGF R1 is one of the five receptor tyrosine kinases (RTKs) (VEGF R1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGF R1/Flt-1, VEGF R2/KDR/Flk-1, VEGF R3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGF R1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGF R1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGF R1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGF R1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

References

He, Y. et al. (1999) Molecular Endocrinology 13:537.
Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Alternate Names

EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

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Citations for Mouse VEGFR1/Flt-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Microglial control of astrocytes in response to microbial metabolites
Authors: Rothhammer V, Borucki DM, Tjon EC et al.
Nature
Endothelial cell signature in muscle stem cells validated by VEGFA-FLT1-AKT1 axis promoting survival of muscle stem cell
Authors: Verma, M;Asakura, Y;Wang, X;Zhou, K;Ünverdi, M;Kann, AP;Krauss, RS;Asakura, A;
eLife
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Tumor Microenvironment Modulates Immunological Outcomes of Myeloid Cells with mTORC1 Disruption
Authors: C Ding, X Sun, C Wu, X Hu, HG Zhang, J Yan
J. Immunol., 2019-01-21;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Antifibrotic role of vascular endothelial growth factor in pulmonary fibrosis
Authors: LA Murray, DM Habiel, M Hohmann, A Camelo, H Shang, Y Zhou, AL Coelho, X Peng, M Gulati, B Crestani, MA Sleeman, T Mustelin, MW Moore, C Ryu, AD Osafo-Addo, JA Elias, CG Lee, B Hu, JD Herazo-May, DA Knight, CM Hogaboam, EL Herzog
JCI Insight, 2017-08-17;2(16):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.
Authors: Coffelt S, Kersten K, Doornebal C, Weiden J, Vrijland K, Hau C, Verstegen N, Ciampricotti M, Hawinkels L, Jonkers J, de Visser K
Nature, 2015-03-30;522(7556):345-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets.
Authors: Weston W, Zayas J, Perez R, George J, Jurecic R
Sci Rep, 2014-06-06;4(0):5199.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry