The Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel is designed for the identification and characterization of human, mouse, or rat neural progenitor cells by marker expression using immunocytochemistry and flow cytometry techniques.
*This kit requires secondary development reagents for immunocytochemistry, such as the NorthernLights™ Fluorescent Secondary Antibodies, and flow cytometry, such as anti-mouse IgG (Catalog # F0101B, F0102B, F0103B, or F0114) and anti-mouse IgM (Catalog # F0116, F0117, F0118, or F0119).
Detection of Neural Progenitor Cells using the Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel. A) Rat cortical stem cells (Catalog # NSC001) or B) mouse cortical stem cells (Catalog # NSC002) were stained with anti-Nestin (A) or anti-Musashi-1 (B) antibodies included in the Human/Mouse/Rat Neural Progenitor Cell Marker Antibody Panel (Catalog # SC025). Cells in (A) were subsequently stained with NorthernLights™ 557-conjugated Donkey Anti-Mouse Secondary Antibody (Catalog # NL007; red). Cells in (B) were subsequently stained with NorthernLights 493-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # NL003; green) and counterstained with DAPI (blue). C, D) Flow cytometry was also used to detect rat cortical stem cells (C) or mouse cortical stem cells (D) stained with anti-SSEA-1 (C) or anti-CXCR4 (D) antibodies (filled histograms) included in the Human/MouseRat Neural Progenitor Cell Marker Antibody Panel (Catalog # SC025). Open histograms show staining with the appropriate isotype controls. |