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Goat IgG NorthernLights™ NL493-conjugated Antibody

Catalog #: NL003
16 Citations Datasheet / COA / SDS
NorthernLights 493 Fluorochrome-labeled Antibody, X Absorbed
Catalog # Availability Size / Price Qty
NL003
Detection of Goat IgG Primary Antibody by Immunocytochemistry.
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Product Details
Citations (16)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Goat IgG NorthernLights™ NL493-conjugated Antibody Summary

Species Reactivity
Goat
Specificity
Goat IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Goat IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL493 (Excitation= 493 nm, Emission= 514 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Goat IgG Primary Antibody by Immunocytochemistry. Detection of human EGFR by incubating cells with 5 μg/mL of Goat anti-Human EGFR polyclonal antibody (AF231) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A431 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Goat IgG NorthernLights™ NL493-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Generation of a human induced pluripotent stem cell line from a patient with GM3 synthase deficiency using self-replicating RNA vector
    Authors: Tonin, R;Feo, F;Falliano, S;Giunti, L;Calamai, M;Procopio, E;Mari, F;Sciruicchio, V;Conti, V;Fanelli, I;Bambi, F;Guerrini, R;Morrone, A;
    Stem cell research
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  2. Engineered Interleukin-6-derived cytokines recruit artificial receptor complexes and disclose CNTF signaling via the OSMR
    Authors: Rafii, P;Cruz, PR;Ettich, J;Seibel, C;Padrini, G;Wittich, C;Lang, A;Petzsch, P;Köhrer, K;Moll, JM;Floss, DM;Scheller, J;
    The Journal of biological chemistry
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Humoral and Cellular Immune Responses Induced by Bivalent DNA Vaccines Expressing Fusion Capsid Proteins of Porcine Circovirus Genotypes 2a and 2b
    Authors: Meas, S;Chaimongkolnukul, K;Narkpuk, J;Mekvichitsaeng, P;Poomputsa, K;Wanasen, N;Roshorm, YM;
    Vaccines
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  4. Ultrasound-guided Photoacoustic image Annotation Toolkit in MATLAB (PHANTOM) for preclinical applications
    Authors: Sweeney, A;Arora, A;Edwards, S;Mallidi, S;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. A Hybrid Soluble gp130/Spike-Nanobody Fusion Protein Simultaneously Blocks Interleukin-6 trans -Signaling and Cellular Infection with SARS-CoV-2
    Authors: Julia Ettich, Julia Werner, Hendrik T. Weitz, Eva Mueller, Roland Schwarzer, Philipp A. Lang et al.
    Journal of Virology
  6. Upregulation of Neuroprogenitor and Neural Markers via Enforced miR-124 and Growth Factor Treatment
    Authors: Ameneh Mehri Ghahfarrokhi, Mohammad Saeid Jami, Morteza Hashemzadeh Chaleshtori
    Int J Mol Cell Med
  7. The functions of azurin of Pseudomonas aeruginosa and human mammaglobin-A on proapoptotic and cell cycle regulatory genes expression in the MCF-7 breast cancer cell line
    Authors: P Ghasemi-De, A Doosti, MS Jami
    Saudi journal of biological sciences, 2020-04-14;27(9):2308-2317.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  8. Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA
    Authors: C Miyagi-Shi, Y Nakashima, N Kobayashi, I Saitoh, M Watanabe, H Noguchi
    Sci Rep, 2018-08-17;8(1):12341.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Bone morphogenetic protein-9 promotes the differentiation of mouse spleen macrophages into osteoclasts via the ALK1 receptor and ERK 1/2 pathways in vitro
    Authors: Hai Li, Dahang Zhao, Shengjing Wang, Jing Ding, Li Zhao
    Molecular Medicine Reports
  10. Inflammatory regulatory T cells in the microenvironments of ulcerative colitis and colon carcinoma
    Authors: I Kryczek, L Wang, K Wu, W Li, E Zhao, T Cui, S Wei, Y Liu, Y Wang, L Vatan, W Szeliga, JK Greenson, J Roli?ski, W Zgodzinski, E Huang, K Tao, G Wang, W Zou
    Oncoimmunology, 2016-08-12;5(8):e1105430.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Homeostatic dysregulation in membrane properties of masticatory motoneurons compared with oculomotor neurons in a mouse model for amyotrophic lateral sclerosis.
    Authors: Venugopal S, Hsiao C, Sonoda T, Wiedau-Pazos M, Chandler S
    J Neurosci, 2015-01-14;35(2):707-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Scavenger receptor-BI is a receptor for lipoprotein(a).
    Authors: Yang X, Amar M, Vaisman B, Bocharov A, Vishnyakova T, Freeman L, Kurlander R, Patterson A, Becker L, Remaley A
    J Lipid Res, 2013-06-29;54(9):2450-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. Identification of galectin-1 as a critical factor in function of mouse mesenchymal stromal cell-mediated tumor promotion.
    Authors: Szebeni G, Kriston-Pal E, Blazso P, Katona R, Novak J, Szabo E, Czibula A, Fajka-Boja R, Hegyi B, Uher F, Krenacs L, Joo G, Monostori E
    PLoS ONE, 2012-07-23;7(7):e41372.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells.
    Authors: Gopalakrishna-Pillai S, Iverson LE
    PLoS ONE, 2011-06-15;6(6):e20663.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Tumor necrosis factor receptor expression and signaling in renal cell carcinoma.
    Authors: Al-Lamki RS, Sadler TJ, Wang J
    Am. J. Pathol., 2010-06-21;177(2):943-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Standard opioid agonists activate heteromeric opioid receptors: evidence for morphine and [d-Ala(2)-MePhe(4)-Glyol(5)]enkephalin as selective μ-δ agonists
    Authors: Ajay S. Yekkirala, Alexander E. Kalyuzhny, Philip S. Portoghese
    ACS Chemical Neuroscience

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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