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Neuron-specific beta-III Tubulin APC-conjugated Antibody

Catalog #: IC1195A
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC1195A
Detection of beta ‑III Tubulin in A172 cells by Flow Cytometry
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Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Neuron-specific beta-III Tubulin APC-conjugated Antibody Summary

Specificity
Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # TuJ-1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Rat brain-derived microtubules
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
A172 human glioblastoma cell line and Eight-day differentiated Rat cortical stem cells fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of beta ‑III Tubulin in A172 cells by Flow Cytometry A172 cells were stained with Mouse Anti-Neuron-specific beta ‑III Tubulin APC‑conjugated Monoclonal Antibody (Catalog # IC1195A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Saponin. View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of beta -III Tubulin antibody in Rat Cortical Stem Cells antibody by Flow Cytometry. View Larger

Detection of beta ‑III Tubulin in Rat Cortical Stem Cells by Flow Cytometry. Eight-day differentiated Rat cortical stem cells was stained with Mouse Anti-beta -III Tubulin APC-conjugated Monoclonal Antibody (Catalog # IC1195A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: beta-III Tubulin

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal neuronal commitment in primitive neuroepithelium.

Entrez Gene IDs
10381 (Human)
Alternate Names
Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen

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Citations for Neuron-specific beta-III Tubulin APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. COUP-TF1 Modulates Thyroid Hormone Action in an Embryonic Stem-Cell Model of Cortical Pyramidal Neuronal Differentiation
    Authors: Xiaochun Teng, Yan-Yun Liu, Weiping Teng, Gregory A. Brent
    Thyroid
  2. Characterization of an induced pluripotent stem cell line (IMBPASi001-A) derived from fibroblasts of a patient affected by Wolfram Syndrome
    Authors: DP Grzela, B Marciniak, L Pulaski
    Stem Cell Res, 2020-06-01;46(0):101858.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC

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