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Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

Catalog #: IC3570F
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
IC3570F
Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry.
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Citations (3)
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Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGF R phosphorylated at Y1068 in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 338324
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing human EGF R Y1068 site
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of EGF R/ErbB1 antibody in A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line treated with recombinant human EGF (Catalog # 236-EG) was stained with Mouse Anti-Human Phospho-EGF R/ErbB1 (Y1068) Fluorescein-conjugated Monoclonal Antibody (Catalog # IC3570F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: EGFR

Epidermal growth factor receptor (EGF R, also known as ErbB1 and HER1) is the founding member of the ErbB family of receptor tyrosine kinases. Ligand binding induces receptor dimerization and autophosphorylation on multiple tyrosine residues. Phosphorylation at Tyr 1068 allows binding of the SH2 domain of the cytosolic adaptor Grb2. This binding results in Ras activation.

Long Name
Epidermal Growth Factor Receptor
Entrez Gene IDs
1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)
Alternate Names
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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Citations for Phospho-EGFR (Y1068) Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Effect of Epigallocatechin-3-Gallate on EGFR Signaling and Migration in Non-Small Cell Lung Cancer
    Authors: Cristina Minnelli, Laura Cianfruglia, Emiliano Laudadio, Giovanna Mobbili, Roberta Galeazzi, Tatiana Armeni
    International Journal of Molecular Sciences
  2. In situ neutrophil efferocytosis shapes T cell immunity to influenza infection
    Authors: K Lim, TH Kim, A Trzeciak, AM Amitrano, EC Reilly, H Prizant, DJ Fowell, DJ Topham, M Kim
    Nat. Immunol., 2020-08-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Loss of N-Acetylgalactosaminyltransferase-4 Orchestrates Oncogenic MicroRNA-9 in Hepatocellular Carcinoma
    Authors: Y Liu, H Liu, L Yang, Q Wu, W Liu, Q Fu, W Zhang, H Zhang, J Xu, J Gu
    J. Biol. Chem, 2017-01-06;292(8):3186-3200.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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