Porcine IFN-gamma DuoSet ELISA

Catalog #: DY985
12 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY985

Ancillary Products Available

DY008B: DuoSet ELISA Ancillary Reagent Kit 2

1 Image

All IFN-gamma ELISAs

Product Details

Citations (12)

Supplemental Products

Reviews

Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Porcine IFN-gamma DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

62.5 - 4,000 pg/mL

Sufficient Materials

For fifteen 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant porcine IFN-gamma. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

View Larger

Porcine IFN-gamma ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name:

Interferon gamma

Entrez Gene IDs:

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Alternate Names:

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Citations for Porcine IFN-gamma DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
Filter your results:

Filter by:

Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1
Authors: Pedrera, M;Soler, A;Simón, A;Casado, N;Pérez, C;García-Casado, MA;Fernández-Pacheco, P;Sánchez-Cordón, PJ;Arias, M;Gallardo, C;
Vaccines
Species: Porcine
Sample Types: Serum
A Genetically Engineered Bivalent Vaccine Coexpressing a Molecular Adjuvant against Classical Swine Fever and Porcine Epidemic Diarrhea
Authors: Wang, H;Yi, W;Qin, H;Wang, Q;Guo, R;Pan, Z;
International journal of molecular sciences
Species: Porcine
Sample Types: Cell Culture Supernates
Effect of dietary beta-glucan on intestinal microbial diversity and Salmonella vaccine immunogenicity and efficacy in pigs
Authors: CL Loving, SMD Bearson, BL Bearson, BJ Kerr, TG Kiros, DC Shippy, JM Trachsel
Veterinary Microbiology, 2022-12-28;278(0):109648.
Species: Porcine
Sample Types: Cell Culture Supernates
Cross-Species Transmission of Rabbit Hepatitis E Virus to Pigs and Evaluation of the Protection of a Virus-like Particle Vaccine against Rabbit Hepatitis E Virus Infection in Pigs
Authors: SH Han, HS Ahn, HJ Go, DH Kim, DY Kim, JH Kim, KB Lim, JB Lee, SY Park, CS Song, SW Lee, YK Choi, IS Choi
Vaccines, 2022-06-30;10(7):.
Species: Porcine
Sample Types: Serum
Early Immune Initiation by Porcine Cells following Toxoplasma gondii Infection versus TLR Ligation
Authors: B Hamid, J Schlosser-, L Bechtold, F Ebner, S Rausch, S Hartmann
Microorganisms, 2021-08-28;9(9):.
Species: Porcine
Sample Types: Cell Culture Supernates
Antiviral effects of ergosterol peroxide in a pig model of porcine deltacoronavirus (PDCoV) infection involves modulation of apoptosis and tight junction in the small intestine
Authors: C Duan, J Wang, Y Liu, J Zhang, J Si, Z Hao, J Wang
Veterinary research, 2021-06-14;52(1):86.
Species: Porcine
Sample Types: Serum
The Effect of Zearalenone on the Cytokine Environment, Oxidoreductive Balance and Metabolism in Porcine Ileal Peyer's Patches
Authors: K Obremski, W Trybowski, P Wojtacha, M Gaj?cka, J Tyburski, ? Zielonka
Toxins (Basel), 2020-05-27;12(6):.
Species: Porcine
Sample Types: Whole Tissue
Capsulized faecal microbiota transplantation ameliorates post-weaning diarrhoea by modulating the gut microbiota in piglets
Authors: W Tang, D Chen, B Yu, J He, Z Huang, P Zheng, X Mao, Y Luo, J Luo, Q Wang, H Wang, J Yu
Vet. Res., 2020-04-16;51(1):55.
Species: Porcine
Sample Types: Tissue Homogenates
Addition of dairy lipids and probiotic Lactobacillus fermentum in infant formula programs gut microbiota and entero-insular axis in adult minipigs
Authors: M Lemaire, S Dou, A Cahu, M Formal, L Le Normand, V Romé, I Nogret, S Ferret-Ber, M Rhimi, I Cuinet, C Canlet, M Tremblay-F, P Le Ruyet, C Baudry, P Gérard, I Le Huërou-, S Blat
Sci Rep, 2018-08-03;8(1):11656.
Species: Human
Sample Types: Cell Culture Supernates
Experimental Porcine Toxoplasma gondii Infection as a Representative Model for Human Toxoplasmosis
Authors: J Nau, SK Eller, J Wenning, KH Spekker-Bo, H Schroten, C Schwerk, A Hotop, U Gro beta, W Däubener
Mediators Inflamm., 2017-08-13;2017(0):3260289.
Species: Porcine
Sample Types: Cell Culture Supernates

Early Gut Microbiota Intervention Suppresses DSS-Induced Inflammatory Responses by Deactivating TLR/NLR Signalling in Pigs
Authors: Y Xiao, H Yan, H Diao, B Yu, J He, J Yu, P Zheng, X Mao, Y Luo, D Chen
Sci Rep, 2017-06-12;7(1):3224.
Species: Porcine
Sample Types: Tissue Culture Supernates

The immune response against Chlamydia suis genital tract infection partially protects against re-infection.
Authors: De Clercq E, Devriendt B, Yin L, Chiers K, Cox E, Vanrompay D
Vet Res, 2014-09-25;45(0):95.
Species: Porcine
Sample Types: Cell Culture Supernates