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Porcine IL-6 Quantikine ELISA Kit

Catalog #: P6000B
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Porcine IL-6 ELISA Standard Curve
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Porcine IL-6 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
4.3 pg/mL
Assay Range
18.8 - 1,200 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant porcine IL-6
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Porcine IL-6 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant porcine IL-6 and antibodies raised against the recombinant factor. The immunoassay has been shown to accurately quantitate recombinant porcine IL-6. Results obtained using natural porcine IL-6 show dose response curves that are parallel to the standard curves obtained using recombinant Quantikine Porcine IL-6 kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-6.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 54.5 123 674 52.9 127 665
Standard Deviation 2.8 5.4 17.6 3.9 7.5 30.1
CV% 5.1 4.4 2.6 7.4 5.9 4.5

Recovery

The recovery of porcine IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 102 95-109
EDTA Plasma (n=4) 97 89-104
Heparin Plasma (n=4) 97 85-103
Serum (n=4) 96 87-110

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of porcine IL-6 in each matrix were diluted with Calibrator Diluent and then assayed.
Porcine IL-6 ELISA Linearity

Scientific Data

N/A Porcine IL-6 ELISA Standard Curve View Larger

Porcine IL-6 ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic, alpha -helical, 22 - 28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature porcine IL-6 is 183 amino acids (aa) in length and shares 58%, 39% and 39% aa sequence identity with human, mouse, and rat IL-6, respectively (6). Cells known to express IL-6 include CD8+ T cells, fibroblasts, synoviocytes, adipocytes, osteoblasts, megakaryocytes, endothelial cells (under the influence of endothelins), sympathetic neurons, cerebral cortex neurons, adrenal medulla chromaffin cells, retinal pigment cells, mast cells, keratinocytes, Langerhans cells, fetal and adult astrocytes, neutrophils, monocytes, eosinophils, colonic epithelial cells, B1 B cells and pancreatic islet beta cells (2, 7-29). IL-6 production is generally correlated with cell activation and is normally kept in control by glucocorticoids, catecholamines, and secondary sex steroids (2). Normal human circulating IL-6 is in the 1 pg/mL range, with slight elevations during the menstrual cycle, modest elevations in certain cancers, and large elevations after surgery (30-34). 

IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 Ra) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130, and gp130 dimerization (35). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (36). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130- expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 37). 
IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response. IL-6 is almost solely responsible for fever and the acute phase response in the liver, and it is important in the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in conditions such as obesity, insulin resistance, inflammatory bowel disease, arthritis, and sepsis (2, 5). IL-6 modulates bone resorption and is a major effector of inflammatory joint destruction in rheumatoid arthritis through its promotion of Th17 cell development and activity (1). It contributes to atherosclerotic plaque development and destabilization as well as the development of inflammation-associated carcinogenesis (1, 2). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of memory B cells and plasma cells (38).

Long Name:
Interleukin 6
Entrez Gene IDs:
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names:
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Porcine IL-6 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

66 Citations: Showing 1 - 10
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  1. Analysis of the Model of Atherosclerosis Formation in Pig Hearts as a Result of Impaired Activity of DNA Repair Enzymes
    Authors: Paslawski, R;Kowalczyk, P;Paslawska, U;Wi?niewski, J;Dzi?giel, P;Janiszewski, A;Kiczak, L;Zacharski, M;Gawdzik, B;Kramkowski, K;Szuba, A;
    International journal of molecular sciences
    Species: Porcine
    Sample Types: Serum
  2. Porcine circovirus type 2 infection inhibits macrophage M1 polarization induced by other pathogens via viral capsid protein and host gC1qR protein
    Authors: Yang, X;Du, Q;Wang, X;Shi, J;Wang, T;Li, P;Zhong, J;Tong, D;Huang, Y;
    Veterinary microbiology
    Species: Porcine
    Sample Types: Cell Culture Supernates
  3. Aqueous cannabidiol ?-cyclodextrin complexed polymeric micelle nasal spray to attenuate in vitro and ex vivo SARS-CoV-2-induced cytokine storms
    Authors: Changsan, N;Sawatdee, S;Suedee, R;Chunhachaichana, C;Srichana, T;
    International journal of pharmaceutics
    Species: Porcine
    Sample Types: Tissue Homogenates
  4. Mutating both relA and spoT of enteropathogenic Escherichia coli E2348/69 attenuates its virulence and induces interleukin 6 in vivo
    Authors: JB Lee, SK Kim, D Han, JW Yoon
    Frontiers in Microbiology, 2023-03-02;14(0):1121715.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  5. Phytogenic feed additives alleviate pathogenic Escherichia coli-induced intestinal damage through improving barrier integrity and inhibiting inflammation in weaned pigs
    Authors: SY Chang, MH Song, JH Lee, HJ Oh, YJ Kim, JW An, YB Go, DC Song, HA Cho, SY Cho, DJ Kim, MS Kim, HB Kim, JH Cho
    Journal of animal science and biotechnology, 2022-09-02;13(1):107.
    Species: Porcine
    Sample Types: Serum
  6. Levosimendan Ameliorates Cardiopulmonary Function but Not Inflammatory Response in a Dual Model of Experimental ARDS
    Authors: R Rissel, M Gosling, J Kamuf, M Renz, R Ruemmler, A Ziebart, EK Hartmann
    Biomedicines, 2022-04-29;10(5):.
    Species: Porcine
    Sample Types: Plasma
  7. Effect of chronic and acute enterotoxigenic E. coli challenge on growth performance, intestinal inflammation, microbiome, and metabolome of weaned piglets
    Authors: JX Boeckman, S Sprayberry, AM Korn, JS Suchodolsk, C Paulk, K Genovese, RR Rech, PR Giaretta, AK Blick, T Callaway, JJ Gill
    Scientific Reports, 2022-03-23;12(1):5024.
    Species: Porcine
    Sample Types: Serum
  8. Hemosiderin Accumulation in Liver Decreases Iron Availability in Tachycardia-Induced Porcine Congestive Heart Failure Model
    Authors: M Kasztura, L Kiczak, U Pas?awska, J Bania, A Janiszewsk, A Tomaszek, M Zacharski, A Noszczyk-N, R Pas?awski, A Tabi?, P Kuropka, P Dzi?giel, P Ponikowski
    International Journal of Molecular Sciences, 2022-01-18;23(3):.
    Species: Porcine
    Sample Types: Serum
  9. Identification of Virulence Associated Region during Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus during Attenuation In Vitro: Complex Question with Different Strain Backgrounds
    Authors: Y Jiang, W Tong, L Yu, L Li, F Gao, G Li, C Liu, P Chen, Q Shen, Y Zhang, Y Zhou, G Tong
    Viruses, 2021-12-27;14(1):.
    Species: Porcine
    Sample Types: Serum
  10. A novel pre-clinical strategy to deliver antimicrobial doses of inhaled nitric oxide
    Authors: VS Michaelsen, RVP Ribeiro, E Brambate, A Ali, A Wang, L Pires, M Kawashima, Y Zhang, A Gazzalle, S Keshavjee, L Del Sorbo, M Cypel
    PLoS ONE, 2021-10-13;16(10):e0258368.
    Species: Porcine
    Sample Types: Tissue Homogenates
  11. Inhibition of MAVS Aggregation-Mediated Type-I Interferon Signaling by Foot-and-Mouth Disease Virus VP3
    Authors: P Ekanayaka, BH Lee, A Weerawardh, K Chathurang, JH Park, JS Lee
    Viruses, 2021-09-06;13(9):.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  12. A novel large animal model of smoke inhalation-induced acute respiratory distress syndrome
    Authors: PD Leiphrakpa, HR Weber, A McCain, RR Matas, EM Duarte, KL Buesing
    Respiratory Research, 2021-07-07;22(1):198.
    Species: Porcine
    Sample Types: BALF
  13. SOFA Score, Hemodynamics and Body Temperature Allow Early Discrimination between Porcine Peritonitis-Induced Sepsis and Peritonitis-Induced Septic Shock
    Authors: M Al-Obeidal, D Jarkovská, L Valešová, J Horák, J Jedli?ka, L Nalos, J Chvojka, J Švíglerová, J Kuncová, J Beneš, M Mat?jovi?, M Štengl
    Journal of personalized medicine, 2021-02-28;11(3):.
    Species: Porcine
    Sample Types: Plasma
  14. Hemodynamic effects of high frequency oscillatory ventilation with volume guarantee in a piglet model of respiratory distress syndrome
    Authors: J Bhogal, AL Solevåg, M O'Reilly, TF Lee, C Joynt, LK Hornberger, GM Schmölzer, PY Cheung
    PLoS ONE, 2021-02-16;16(2):e0246996.
    Species: Porcine
    Sample Types: Tissue Homogenates
  15. Experimental lung injury induces cerebral cytokine mRNA production in pigs
    Authors: J Kamuf, A Garcia Bar, A Ziebart, K Frauenknec, K Folkert, J Schwab, R Ruemmler, M Renz, D Cana, SC Thal, EK Hartmann
    PeerJ, 2020-12-09;8(0):e10471.
    Species: Porcine
    Sample Types: Whole Blood
  16. Protective effects of new femoral reaming techniques (Reamer irrigator aspirator, RIA I and II) on pulmonary function and posttraumatic contusion (CT morphology) - results from a standardized large animal model
    Authors: S Halvachiza, M Teuben, M Lempert, Y Kalbas, N Cesarovic, M Lipiski, E Benninger, P Cinelli, R Pfeifer, HC Pape
    Injury, 2020-10-06;0(0):.
    Species: Porcine
    Sample Types: BALF
  17. Effects of sustained inflation pressure during�neonatal cardiopulmonary resuscitation of asphyxiated piglets
    Authors: GH Shim, SY Kim, PY Cheung, TF Lee, M O'Reilly, GM Schmölzer
    PLoS ONE, 2020-06-23;15(6):e0228693.
    Species: Porcine
    Sample Types: Tissue Homogenates
  18. Substitution of warthog NF-kappaB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus
    Authors: S McCleary, R Strong, RR McCarthy, JC Edwards, EL Howes, LM Stevens, PJ Sánchez-Co, A Núñez, S Watson, AJ Mileham, SG Lillico, C Tait-Burka, C Proudfoot, M Ballantyne, CBA Whitelaw, F Steinbach, HR Crooke
    Sci Rep, 2020-06-02;10(1):8951.
    Species: Porcine
    Sample Types: Serum
  19. Low Protein-High Carbohydrate Diets Alter Energy Balance, Gut Microbiota Composition and Blood Metabolomics Profile in Young Pigs
    Authors: S Spring, H Premathila, U DeSilva, C Shili, S Carter, A Pezeshki
    Sci Rep, 2020-02-24;10(1):3318.
    Species: Porcine
    Sample Types: Serum
  20. Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model
    Authors: SE Pischke, S Hestenes, HT Johansen, H Fure, JF Bugge, A Espinoza, H Skulstad, T Edvardsen, E Fosse, TE Mollnes, PS Halvorsen, EW Nielsen
    PLoS ONE, 2019-06-27;14(6):e0218624.
    Species: Porcine
    Sample Types: Plasma
  21. Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP-PRRSV-1) infection
    Authors: R Saleri, V Cavalli, L Ferrari, G Ogno, E Canelli, P Martelli, P Borghetti
    Res. Vet. Sci., 2019-04-11;124(0):263-269.
    Species: Porcine
    Sample Types: Plasma
  22. PO2 oscillations induce lung injury and inflammation
    Authors: S Boehme, EK Hartmann, T Tripp, SC Thal, M David, D Abraham, JE Baumgardne, K Markstalle, KU Klein
    Crit Care, 2019-03-27;23(1):102.
    Species: Porcine
    Sample Types: Plasma
  23. Inactivating hepatitis C virus in donor lungs using light therapies during normothermic ex vivo lung perfusion
    Authors: M Galasso, JJ Feld, Y Watanabe, M Pipkin, C Summers, A Ali, R Qaqish, M Chen, RVP Ribeiro, K Ramadan, L Pires, VS Bagnato, C Kurachi, V Cherepanov, G Moonen, A Gazzalle, TK Waddell, M Liu, S Keshavjee, BC Wilson, A Humar, M Cypel
    Nat Commun, 2019-01-29;10(1):481.
    Species: Porcine
    Sample Types: Tissue Homogenates
  24. Medium-Chain Triglycerides Attenuate Liver Injury in Lipopolysaccharide-Challenged Pigs by Inhibiting Necroptotic and Inflammatory Signaling Pathways
    Authors: L Zhang, X Wang, S Chen, S Wang, Z Tu, G Zhang, H Zhu, X Li, J Xiong, Y Liu
    Int J Mol Sci, 2018-11-21;19(11):.
    Species: Porcine
    Sample Types: Serum
  25. Neuroimmunological Implications of Subclinical Lipopolysaccharide from Salmonella Enteritidis
    Authors: A Miko?ajczy, D Z?otkowska
    Int J Mol Sci, 2018-10-22;19(10):.
    Species: Porcine
    Sample Types: Serum
  26. PKD regulates actin polymerization, neutrophil deformability, and transendothelial migration in response to fMLP and trauma
    Authors: C Wille, T Eiseler, ST Langenberg, J Richter, K Mizuno, P Radermache, U Knippschil, M Huber-Lang, T Seufferlei, S Paschke
    J. Leukoc. Biol., 2018-04-14;0(0):.
    Species: Porcine
    Sample Types: Plasma
  27. Inflammatory markers before and after farrowing in healthy sows and in sows affected with postpartum dysgalactia syndrome
    Authors: M Kaiser, M Jacobson, PH Andersen, P Bækbo, JJ Cerón, J Dahl, D Escribano, S Jacobsen
    BMC Vet. Res., 2018-03-12;14(1):83.
    Species: Porcine
    Sample Types: Serum
  28. Sodium lactate improves renal microvascular thrombosis compared to sodium bicarbonate and 0.9% NaCl in a porcine model of endotoxic shock: an experimental randomized open label controlled study
    Authors: T Duburcq, A Durand, A Tournoys, V Gnemmi, V Gmyr, F Pattou, M Jourdain, F Tamion, E Besnier, S Préau, E Parmentier, D Mathieu, J Poissy, R Favory
    Ann Intensive Care, 2018-02-14;8(1):24.
    Species: Porcine
    Sample Types: Serum
  29. Influence of zinc supplementation on immune parameters in weaned pigs
    Authors: V Kloubert, K Blaabjerg, TS Dalgaard, HD Poulsen, L Rink, I Wessels
    J Trace Elem Med Biol, 2018-01-31;0(0):.
    Species: Porcine
    Sample Types: Whole Blood
    Applications: ELISA Capture
  30. Beneficial effects of inhaled nitric oxide with intravenous steroid in an ischemia-reperfusion model involving aortic clamping
    Authors: W Gozdzik, S Zielinski, M Zielinska, K Ratajczak, P Skrzypczak, S Rodziewicz, A Kübler, K Löfström, P Dziegiel, M Olbromski, B Adamik, S Ryniak, P Harbut, J Albert, C Frostell
    Int J Immunopathol Pharmacol, 2018-01-01;32(0):3946320177514.
    Species: Porcine
    Sample Types: Plasma
  31. Septic porcine blood does not further activate coagulation during in vitro membrane oxygenation
    Authors: Rolf Rossaint
    Eur J Cardiothorac Surg, 2017-03-01;0(0):.
    Species: Porcine
    Sample Types: Plasma
  32. Characterization of blunt chest trauma in a long-term porcine model of severe multiple trauma
    Sci Rep, 2016-12-21;6(0):39659.
    Species: Porcine
    Sample Types: Serum
  33. An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome
    Authors: Angela J Westover
    J Diabetes Res, 2016-10-13;2016(0):3486727.
    Species: Porcine
    Sample Types: Plasma
  34. Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression.
    Authors: Clayton K, Douglas-Vail M, Nur-ur Rahman A, Medcalf K, Xie I, Chew G, Tandon R, Lanteri M, Norris P, Deeks S, Ndhlovu L, Ostrowski M
    J Virol, 2015-01-21;89(7):3723-36.
    Species: Porcine
    Sample Types: Plasma
  35. Low tidal volume pressure support versus controlled ventilation in early experimental sepsis in pigs.
    Authors: Ziebart A, Hartmann E, Thomas R, Liu T, Duenges B, Schad A, Bodenstein M, Thal S, David M
    Respir Res, 2014-09-06;15(0):101.
    Species: Porcine
    Sample Types: Plasma
  36. Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig.
    Authors: Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S
    Mol. Ther., 2012-03-27;20(6):1204-11.
    Species: Porcine
    Sample Types: Plasma
  37. Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs.
    Authors: Martinez-Olondris P, Rigol M, Soy D, Guerrero L, Agusti C, Quera MA, Li Bassi G, Esperatti M, Luque N, Liapikou M, Filella X, Marco F, de la Bellacasa JP, Torres A
    Crit. Care Med., 2012-01-01;40(1):162-8.
    Species: Porcine
    Sample Types: Serum
  38. Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.
    Authors: Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P
    Crit. Care Med., 2010-06-01;38(6):1467-74.
    Species: Porcine
    Sample Types: Plasma
  39. Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
    Authors: Jeong HJ, Song YJ, Lee SW
    Clin. Vaccine Immunol., 2010-02-03;17(4):503-12.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  40. Mutations in classical swine fever virus NS4B affect virulence in swine.
    Authors: Fernandez-Sainz I, Gladue DP, Holinka LG
    J. Virol., 2009-11-18;84(3):1536-49.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  41. Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine.
    Authors: McILwain RB, Timpa JG, Kurundkar AR
    Lab. Invest., 2009-11-09;90(1):128-39.
    Species: Porcine
    Sample Types: Plasma
  42. Cardiac Function and the Proinflammatory Cytokine Response After Recovery From Cardiac Arrest in Swine.
    Authors: Niemann JT, Rosborough JP, Youngquist S, Shah AP, Lewis RJ, Phan QT, Filler SG
    J. Interferon Cytokine Res., 2009-11-01;0(0):.
    Species: Porcine
    Sample Types: Plasma
  43. CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
    Authors: Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE
    FASEB J., 2009-10-19;24(3):712-22.
    Species: Porcine
    Sample Types: Plasma
  44. Bbeta15-42 (FX06) reduces pulmonary, myocardial, liver, and small intestine damage in a pig model of hemorrhagic shock and reperfusion.
    Authors: Roesner JP, Petzelbauer P, Koch A, Tran N, Iber T, Vagts DA, Scheeren TW, Vollmar B, Noldge-Schomburg GE, Zacharowski K
    Crit. Care Med., 2009-02-01;37(2):598-605.
    Species: Porcine
    Sample Types: Plasma
  45. Effect of linezolid compared with glycopeptides in methicillin-resistant Staphylococcus aureus severe pneumonia in piglets.
    Authors: Luna CM, Bruno DA, Garcia-Morato J, Mann KC, Risso Patron J, Sagardia J, Absi R, Garcia Bottino M, Marchetti D, Famiglietti A, Baleztena M, Biancolini C
    Chest, 2009-01-13;135(6):1564-71.
    Species: Porcine
    Sample Types: Serum
  46. Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
    Authors: Thorgersen EB, Pharo A, Haverson K, Axelsen AK, Gaustad P, Kotwal GJ, Sfyroera G, Mollnes TE
    Infect. Immun., 2008-12-01;77(2):725-32.
    Species: Porcine
    Sample Types: Whole Blood
  47. Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.
    Authors: Kruse R, Essen-Gustavsson B, Fossum C, Jensen-Waern M
    Acta Vet. Scand., 2008-08-12;50(0):32.
    Species: Porcine
    Sample Types: Serum
  48. Effects of glucocorticoids in ventilated piglets with severe pneumonia.
    Authors: Sibila O, Luna CM, Agusti C, Baquero S, Gando S, Patron JR, Morato JG, Absi R, Bassi N, Torres A
    Eur. Respir. J., 2008-05-28;32(4):1037-46.
    Species: Porcine
    Sample Types: Serum
  49. Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
    Authors: Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE
    J. Immunol., 2008-05-01;180(9):6346-53.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  50. Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response.
    Authors: Sibila O, Agusti C, Torres A, Baquero S, Gando S, Patron JR, Morato JG, Goffredo DH, Bassi N, Luna CM
    Eur. Respir. J., 2007-09-05;30(6):1167-72.
    Species: Porcine
    Sample Types: Serum
  51. Experimental severe Pseudomonas aeruginosa pneumonia and antibiotic therapy in piglets receiving mechanical ventilation.
    Authors: Luna CM, Baquero S, Gando S, Patron JR, Morato JG, Sibila O, Absi R, Famiglietti A, Vay CA, Von Stecher F, Agusti C, Torres A
    Chest, 2007-08-01;132(2):523-31.
    Species: Porcine
    Sample Types: Serum
  52. The fibrin-derived peptide Bbeta15-42 is cardioprotective in a pig model of myocardial ischemia-reperfusion injury.
    Authors: Roesner JP, Petzelbauer P, Koch A, Mersmann J, Zacharowski PA, Boehm O, Reingruber S, Pasteiner W, Mascher D, Wolzt M, Barthuber C, Noldge-Schomburg GE, Scheeren TW, Zacharowski K
    Crit. Care Med., 2007-07-01;35(7):1730-5.
    Species: Porcine
    Sample Types: Plasma
  53. Sex difference in link between interleukin-6 and stress.
    Authors: Jankord R, Turk JR, Schadt JC, Casati J, Ganjam VK, Price EM, Keisler DH, Laughlin MH
    Endocrinology, 2007-05-17;148(8):3758-64.
    Species: Porcine
    Sample Types: Plasma
  54. Complete overlap of interleukin-31 receptor A and oncostatin M receptor beta in the adult dorsal root ganglia with distinct developmental expression patterns.
    Authors: Bando T, Morikawa Y, Komori T, Senba E
    Neuroscience, 2006-08-22;142(4):1263-71.
    Species: Porcine
    Sample Types: Plasma
  55. Dietary fatty acid composition rather than vitamin E supplementation influence ex vivo cytokine and eicosanoid response of porcine alveolar macrophages.
    Authors: Moller S, Lauridsen C
    Cytokine, 2006-08-17;35(1):6-12.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  56. A novel antidote-controlled anticoagulant reduces thrombin generation and inflammation and improves cardiac function in cardiopulmonary bypass surgery.
    Authors: Nimjee SM, Keys JR, Pitoc GA, Quick G, Rusconi CP, Sullenger BA
    Mol. Ther., 2006-06-09;14(3):408-15.
    Species: Porcine
    Sample Types: Plasma
  57. Effects of therapeutic bronchoalveolar lavage and partial liquid ventilation on meconium-aspirated newborn piglets.
    Authors: Jeng MJ, Soong WJ, Lee YS, Chang HL, Shen CM, Wang CH, Yang SS, Hwang B
    Crit. Care Med., 2006-04-01;34(4):1099-105.
    Species: Porcine
    Sample Types: Serum
  58. Effects of heparin-binding protein (CAP37/azurocidin) in a porcine model of Actinobacillus pleuropneumoniae-induced pneumonia.
    Authors: Lauritzen B, Lykkesfeldt J, Djurup R, Flodgaard H, Svendsen O
    Pharmacol. Res., 2005-06-01;51(6):509-14.
    Species: Porcine
    Sample Types: Plasma
  59. Protection against acute porcine lung ischemia/reperfusion injury by systemic preconditioning via hind limb ischemia.
    Authors: Waldow T, Alexiou K, Witt W, Albrecht S, Wagner F, Knaut M, Matschke K
    Transpl. Int., 2005-02-01;18(2):198-205.
    Species: Porcine
    Sample Types: Plasma
  60. Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide.
    Authors: Changhua L, Jindong Y, Defa L, Lidan Z, Shiyan Q, Jianjun X
    J. Nutr., 2005-02-01;135(2):239-44.
    Species: Porcine
    Sample Types: Serum
  61. Decreased protein accretion in pigs with viral and bacterial pneumonia is associated with increased myostatin expression in muscle.
    Authors: Escobar J, Van Alstine WG, Baker DH
    J. Nutr., 2004-11-01;134(11):3047-53.
    Species: Porcine
    Sample Types: Serum
  62. Attenuation of reperfusion-induced systemic inflammation by preconditioning with nitric oxide in an in situ porcine model of normothermic lung ischemia.
    Authors: Waldow T, Alexiou K, Witt W, Wagner FM, Gulielmos V, Matschke K, Knaut M
    Chest, 2004-06-01;125(6):2253-9.
    Species: Porcine
    Sample Types: Plasma
  63. Adiponectin differentially regulates cytokines in porcine macrophages.
    Authors: Wulster-Radcliffe MC, Ajuwon KM, Wang J, Christian JA, Spurlock ME
    Biochem. Biophys. Res. Commun., 2004-04-09;316(3):924-9.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  64. Classical swine fever virus induces proinflammatory cytokines and tissue factor expression and inhibits apoptosis and interferon synthesis during the establishment of long-term infection of porcine vascular endothelial cells.
    Authors: Bensaude E, Turner JL, Wakeley PR, Sweetman DA, Pardieu C, Drew TW, Wileman T, Powell PP
    J. Gen. Virol., 2004-04-01;85(0):1029-37.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  65. Standardized lung recruitment during high frequency and conventional ventilation: similar pathophysiologic and inflammatory responses in an animal model of respiratory distress syndrome.
    Authors: Krishnan RK, Meyers PA, Worwa C, Goertz R, Schauer G, Mammel MC
    Intensive Care Med, 2004-03-02;30(6):1195-203.
    Species: Porcine
    Sample Types: Serum
  66. Chronic porcine two-hit model with hemorrhagic shock and Pseudomonas aeruginosa sepsis.
    Authors: Eissner B, Matz K, Smorodchenko A, Roschmann A, v Specht BU
    Eur Surg Res, 2002-01-01;34(1):61-7.
    Species: Porcine
    Sample Types: Plasma

FAQs

  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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Porcine IL-6 Quantikine ELISA Kit
By Anonymous on 12/11/2021
Sample Tested: Serum
Porcine IL-6 Quantikine ELISA Kit P6000B