Porcine TNF-alpha Quantikine ELISA Kit Summary
Product Summary
Recovery
The recovery of porcine TNF-alpha spiked to three levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Supernates (n=6) | 99 | 91-105 |
EDTA Plasma (n=6) | 98 | 83-111 |
Heparin Plasma (n=4) | 91 | 82-99 |
Serum (n=6) | 105 | 91-120 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: TNF-alpha
Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD). Within the ECD, human TNF-α shares 97% aa sequence identity with rhesus and 71% - 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness.
Cleavage of membrane bound TNF-α by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Porcine TNF-alpha Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Necroptosis contributes to the intestinal toxicity of deoxynivalenol and is mediated by methyltransferase SETDB1
Authors: Zhou, B;Xiao, K;Guo, J;Xu, Q;Xu, Q;Lv, Q;Zhu, H;Zhao, J;Liu, Y;
Journal of hazardous materials
Species: Porcine
Sample Types: Cell Culture Supernates
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Supplementation of vitamin E or a botanical extract as antioxidants to improve growth performance and health of growing pigs housed under thermoneutral or heat-stressed conditions
Authors: Silva-Guillen, YV;Arellano, C;Wiegert, J;Boyd, RD;Martínez, GE;van Heugten, E;
Journal of animal science and biotechnology
Species: Porcine
Sample Types: Tissue Homogenates
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Analysis of the Model of Atherosclerosis Formation in Pig Hearts as a Result of Impaired Activity of DNA Repair Enzymes
Authors: Paslawski, R;Kowalczyk, P;Paslawska, U;Wi?niewski, J;Dzi?giel, P;Janiszewski, A;Kiczak, L;Zacharski, M;Gawdzik, B;Kramkowski, K;Szuba, A;
International journal of molecular sciences
Species: Porcine
Sample Types: Serum
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Early weaning and biological sex shape long-term immune and metabolic responses in pigs
Authors: Fardisi, M;Thelen, K;Groenendal, A;Rajput, M;Sebastian, K;Contreras, GA;Moeser, AJ;
Scientific reports
Species: Porcine
Sample Types: Plasma
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Porcine circovirus type 2 infection inhibits macrophage M1 polarization induced by other pathogens via viral capsid protein and host gC1qR protein
Authors: Yang, X;Du, Q;Wang, X;Shi, J;Wang, T;Li, P;Zhong, J;Tong, D;Huang, Y;
Veterinary microbiology
Species: Porcine
Sample Types: Cell Culture Supernates
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Efficacy of Feed Additive Containing Bentonite and Enzymatically Hydrolyzed Yeast on Intestinal Health and Growth of Newly Weaned Pigs under Chronic Dietary Challenges of Fumonisin and Aflatoxin
Authors: Deng, Z;Jang, KB;Jalukar, S;Du, X;Kim, SW;
Toxins
Species: Porcine
Sample Types: Tissue Homogenates
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Aqueous cannabidiol ?-cyclodextrin complexed polymeric micelle nasal spray to attenuate in vitro and ex vivo SARS-CoV-2-induced cytokine storms
Authors: Changsan, N;Sawatdee, S;Suedee, R;Chunhachaichana, C;Srichana, T;
International journal of pharmaceutics
Species: Porcine
Sample Types: Tissue Homogenates
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Regenerative Effect of Mesenchymal Stem Cell on Cartilage Damage in a Porcine Model
Authors: Lin, S;Panthi, S;Hsuuw, Y;Chen, S;Huang, M;Sieber, M;Hsuuw, Y;
Biomedicines
Species: Porcine
Sample Types: Serum
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Phytogenic feed additives alleviate pathogenic Escherichia coli-induced intestinal damage through improving barrier integrity and inhibiting inflammation in weaned pigs
Authors: SY Chang, MH Song, JH Lee, HJ Oh, YJ Kim, JW An, YB Go, DC Song, HA Cho, SY Cho, DJ Kim, MS Kim, HB Kim, JH Cho
Journal of animal science and biotechnology, 2022-09-02;13(1):107.
Species: Porcine
Sample Types: Serum
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Supplementation With Spirulina Reduces Infarct Size and Ameliorates Cardiac Function in a Pig Model of STEMI
Authors: G Vilahur, P Sutelman, S Ben-Aicha, G Mendieta, M Radiké, L Schoch, L Casaní, M Borrell-Pa, T Padro, L Badimon
Frontiers in Pharmacology, 2022-05-03;13(0):891801.
Species: Porcine
Sample Types: Plasma
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Levosimendan Ameliorates Cardiopulmonary Function but Not Inflammatory Response in a Dual Model of Experimental ARDS
Authors: R Rissel, M Gosling, J Kamuf, M Renz, R Ruemmler, A Ziebart, EK Hartmann
Biomedicines, 2022-04-29;10(5):.
Species: Porcine
Sample Types: Plasma
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Impacts of weaning weights and mycotoxin challenges on jejunal mucosa-associated microbiota, intestinal and systemic health, and growth performance of nursery pigs
Authors: DM Holanda, SW Kim
Journal of animal science and biotechnology, 2022-04-13;13(1):43.
Species: Porcine
Sample Types: Complex Sample Type
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Assessment of the Effects of the Synbiotic Combination of Bifidobacterium longum subsp. infantis CECT 7210 and Oligofructose-Enriched Inulin Against Digestive Bacterial Infections in a Piglet Model
Authors: A Rodríguez-, L Castillejo, P López-Colo, G Cifuentes-, JA Moreno-Muñ, SM Martín-Orú
Frontiers in Microbiology, 2022-03-08;13(0):831737.
Species: Porcine
Sample Types: Serum
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Hemosiderin Accumulation in Liver Decreases Iron Availability in Tachycardia-Induced Porcine Congestive Heart Failure Model
Authors: M Kasztura, L Kiczak, U Pas?awska, J Bania, A Janiszewsk, A Tomaszek, M Zacharski, A Noszczyk-N, R Pas?awski, A Tabi?, P Kuropka, P Dzi?giel, P Ponikowski
International Journal of Molecular Sciences, 2022-01-18;23(3):.
Species: Porcine
Sample Types: Serum
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Influence of rosuvastatin treatment on cerebral inflammation and nitro-oxidative stress in experimental lung injury in pigs
Authors: J Kamuf, A Garcia Bar, A Ziebart, R Ruemmler, J Schwab, M Dib, A Daiber, SC Thal, EK Hartmann
BMC anesthesiology, 2021-09-13;21(1):224.
Species: Porcine
Sample Types: Serum
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IFN-gamma Licensing Does Not Enhance the Reduced Immunomodulatory Potential and Migratory Ability of Differentiation-Induced Porcine Bone Marrow-Derived Mesenchymal Stem Cells in an In Vitro Xenogeneic Application
Authors: HJ Lee, HD Kim, CH Jo, EY Bok, SB Kim, SL Lee, M Jang, SG Bae, SH Yun, SJ Kim, GJ Rho, WJ Lee
BioMed Research International, 2021-09-04;2021(0):4604856.
Species: Porcine
Sample Types: Cell Culture Supernates
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Phytobiotics with Adsorbent to Mitigate Toxicity of Multiple Mycotoxins on Health and Growth of Pigs
Authors: DM Holanda, YI Kim, W Parnsen, SW Kim
Toxins, 2021-06-26;13(7):.
Species: Porcine
Sample Types: Serum
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Epigenetic Modulation of TLR4 Expression by Sulforaphane Increases Anti-Inflammatory Capacity in Porcine Monocyte-Derived Dendritic Cells
Authors: X Qu, C Neuhoff, MU Cinar, M Pröll, E Tholen, D Tesfaye, M Hölker, K Schellande, MJ Uddin
Biology, 2021-05-31;10(6):.
Species: Porcine
Sample Types: Cell Culture Supernates
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Effects of the Administration of Bifidobacterium longum subsp. infantis CECT 7210 and Lactobacillus rhamnosus HN001 and Their Synbiotic Combination With Galacto-Oligosaccharides Against Enterotoxigenic Escherichia coli F4 in an Early Weaned Piglet Model
Authors: A Rodríguez-, L Castillejo, P López-Colo, G Cifuentes-, M Rodríguez-, JA Moreno-Muñ, D Luise, P Trevisi, SM Martín-Orú
Frontiers in Microbiology, 2021-04-16;12(0):642549.
Species: Porcine
Sample Types: Serum
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SOFA Score, Hemodynamics and Body Temperature Allow Early Discrimination between Porcine Peritonitis-Induced Sepsis and Peritonitis-Induced Septic Shock
Authors: M Al-Obeidal, D Jarkovská, L Valešová, J Horák, J Jedli?ka, L Nalos, J Chvojka, J Švíglerová, J Kuncová, J Beneš, M Mat?jovi?, M Štengl
Journal of personalized medicine, 2021-02-28;11(3):.
Species: Porcine
Sample Types: Plasma
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Hemodynamic effects of high frequency oscillatory ventilation with volume guarantee in a piglet model of respiratory distress syndrome
Authors: J Bhogal, AL Solevåg, M O'Reilly, TF Lee, C Joynt, LK Hornberger, GM Schmölzer, PY Cheung
PLoS ONE, 2021-02-16;16(2):e0246996.
Species: Porcine
Sample Types: Tissue Homogenates
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Experimental lung injury induces cerebral cytokine mRNA production in pigs
Authors: J Kamuf, A Garcia Bar, A Ziebart, K Frauenknec, K Folkert, J Schwab, R Ruemmler, M Renz, D Cana, SC Thal, EK Hartmann
PeerJ, 2020-12-09;8(0):e10471.
Species: Porcine
Sample Types: Whole Blood
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Thymol modulates the endocannabinoid system and gut chemosensing of weaning pigs
Authors: A Toschi, B Tugnoli, B Rossi, A Piva, E Grilli
BMC Vet. Res., 2020-08-12;16(1):289.
Species: Porcine
Sample Types: Tissue Homogenates, Tissue Lysates
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Investigation of the Efficacy of a Postbiotic Yeast Cell Wall-Based Blend on Newly-Weaned Pigs under a Dietary Challenge of Multiple Mycotoxins with Emphasis on Deoxynivalenol
Authors: DM Holanda, A Yiannikour, SW Kim
Toxins (Basel), 2020-08-06;12(8):.
Species: Porcine
Sample Types: Cell Culture Supernates
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Hypochlorite-induced porcine model of peritoneal fibrosis through the activation of IL1&beta-CX3CL1-TGF&beta1 signal axis
Authors: YT Hsu, CH Wu, CY Chao, YS Wei, YC Chang, YT Chen, SL Lin, SY Tsai, YJ Lee, PS Tsai
Sci Rep, 2020-07-13;10(1):11496.
Species: Porcine
Sample Types: Serum
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Effects of sustained inflation pressure during�neonatal cardiopulmonary resuscitation of asphyxiated piglets
Authors: GH Shim, SY Kim, PY Cheung, TF Lee, M O'Reilly, GM Schmölzer
PLoS ONE, 2020-06-23;15(6):e0228693.
Species: Porcine
Sample Types: Tissue Homogenates
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Efficacy of Mycotoxin Detoxifiers on Health and Growth of Newly-Weaned Pigs under Chronic Dietary Challenge of Deoxynivalenol
Authors: DM Holanda, SW Kim
Toxins (Basel), 2020-05-09;12(5):.
Species: Porcine
Sample Types: Tissue Homogenates
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Low Protein-High Carbohydrate Diets Alter Energy Balance, Gut Microbiota Composition and Blood Metabolomics Profile in Young Pigs
Authors: S Spring, H Premathila, U DeSilva, C Shili, S Carter, A Pezeshki
Sci Rep, 2020-02-24;10(1):3318.
Species: Porcine
Sample Types: Serum
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Efficacy of medium-chain fatty acid salts distilled from coconut oil against two enteric pathogen challenges in weanling piglets
Authors: P López-Colo, L Castillejo, A Rodríguez-, M Puyalto, JJ Mallo, SM Martín-Orú
J Anim Sci Biotechnol, 2019-11-09;10(0):89.
Species: Porcine
Sample Types: Serum
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Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model
Authors: SE Pischke, S Hestenes, HT Johansen, H Fure, JF Bugge, A Espinoza, H Skulstad, T Edvardsen, E Fosse, TE Mollnes, PS Halvorsen, EW Nielsen
PLoS ONE, 2019-06-27;14(6):e0218624.
Species: Porcine
Sample Types: Plasma
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Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection
Authors: Y Luo, J Xu, C Zhang, C Jiang, Y Ma, H He, Y Wu, B Devriendt, E Cox, H Zhang
Vet. Res., 2019-06-20;50(1):48.
Species: Porcine
Sample Types: Cell Culture Supernates
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Dietary supplementation of xylanase and protease on growth performance, digesta viscosity, nutrient digestibility, immune and oxidative stress status, and gut health of newly weaned pigs
Authors: ME Duarte, FX Zhou, WM Dutra, SW Kim
Anim Nutr, 2019-05-11;5(4):351-358.
Species: Porcine
Sample Types: Serum
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I-FABP, Pig-MAP and TNF-? as biomarkers for monitoring gut-wall integrity in front of Salmonella Typhimurium and ETEC K88 infection in a weaned piglet model
Authors: P López-Colo, K Yu, E Barba-Vida, Y Saco, SM Martín-Orú, L Castillejo, D Solà-Oriol, A Bassols
Res. Vet. Sci., 2019-05-07;124(0):426-432.
Species: Porcine
Sample Types: Serum
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Nonmuscle Myosin Heavy Chain IIA Recognizes Sialic Acids on Sialylated RNA Viruses To Suppress Proinflammatory Responses via the DAP12-Syk Pathway
Authors: Y Liu, R Li, XX Chen, Y Zhi, R Deng, EM Zhou, S Qiao, G Zhang
MBio, 2019-05-07;10(3):.
Species: Porcine
Sample Types: Cell Culture Supernates
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Modulation of the somatotropic axis, adiponectin and cytokine secretion during highly pathogenic porcine reproductive and respiratory syndrome virus type 1 (HP-PRRSV-1) infection
Authors: R Saleri, V Cavalli, L Ferrari, G Ogno, E Canelli, P Martelli, P Borghetti
Res. Vet. Sci., 2019-04-11;124(0):263-269.
Species: Porcine
Sample Types: Plasma
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PO2 oscillations induce lung injury and inflammation
Authors: S Boehme, EK Hartmann, T Tripp, SC Thal, M David, D Abraham, JE Baumgardne, K Markstalle, KU Klein
Crit Care, 2019-03-27;23(1):102.
Species: Porcine
Sample Types: Plasma
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Medium-Chain Triglycerides Attenuate Liver Injury in Lipopolysaccharide-Challenged Pigs by Inhibiting Necroptotic and Inflammatory Signaling Pathways
Authors: L Zhang, X Wang, S Chen, S Wang, Z Tu, G Zhang, H Zhu, X Li, J Xiong, Y Liu
Int J Mol Sci, 2018-11-21;19(11):.
Species: Porcine
Sample Types: Serum
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Neuroimmunological Implications of Subclinical Lipopolysaccharide from Salmonella Enteritidis
Authors: A Miko?ajczy, D Z?otkowska
Int J Mol Sci, 2018-10-22;19(10):.
Species: Porcine
Sample Types: Serum
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Glutamate alleviates intestinal injury, maintains mTOR and suppresses TLR4 and NOD signaling pathways in weanling pigs challenged with lipopolysaccharide
Authors: Q Qin, X Xu, X Wang, H Wu, H Zhu, Y Hou, B Dai, X Liu, Y Liu
Sci Rep, 2018-10-11;8(1):15124.
Species: Porcine
Sample Types: Plasma
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Blood pressure targeting by partial REBOA is possible in severe hemorrhagic shock in pigs and produces less circulatory, metabolic and inflammatory sequelae than total REBOA
Authors: M Sadeghi, TM Hörer, D Forsman, EM Dogan, K Jansson, C Kindler, P Skoog, KF Nilsson
Injury, 2018-09-29;0(0):.
Species: Porcine
Sample Types: Serum
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Pulmonary Effects of Remote Ischemic Preconditioning in a Porcine Model of Ventilation-induced Lung Injury
Authors: A Bergmann, T Schilling, G Hedenstier, K Ahlgren, A Larsson, M Kretzschma, A Kozian, T Hachenberg
Respir Physiol Neurobiol, 2018-08-31;0(0):.
Species: Porcine
Sample Types: Tissue Homogenates
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Inflammatory markers before and after farrowing in healthy sows and in sows affected with postpartum dysgalactia syndrome
Authors: M Kaiser, M Jacobson, PH Andersen, P Bækbo, JJ Cerón, J Dahl, D Escribano, S Jacobsen
BMC Vet. Res., 2018-03-12;14(1):83.
Species: Porcine
Sample Types: Serum
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Medium-chain TAG improve intestinal integrity by suppressing toll-like receptor 4, nucleotide-binding oligomerisation domain proteins and necroptosis signalling in weanling piglets challenged with lipopolysaccharide
Authors: X Xu, S Chen, H Wang, Z Tu, S Wang, X Wang, H Zhu, C Wang, J Zhu, Y Liu
Br. J. Nutr., 2018-03-06;0(0):1-10.
Species: Porcine
Sample Types: Plasma
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Sodium lactate improves renal microvascular thrombosis compared to sodium bicarbonate and 0.9% NaCl in a porcine model of endotoxic shock: an experimental randomized open label controlled study
Authors: T Duburcq, A Durand, A Tournoys, V Gnemmi, V Gmyr, F Pattou, M Jourdain, F Tamion, E Besnier, S Préau, E Parmentier, D Mathieu, J Poissy, R Favory
Ann Intensive Care, 2018-02-14;8(1):24.
Species: Porcine
Sample Types: Serum
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Influence of zinc supplementation on immune parameters in weaned pigs
Authors: V Kloubert, K Blaabjerg, TS Dalgaard, HD Poulsen, L Rink, I Wessels
J Trace Elem Med Biol, 2018-01-31;0(0):.
Species: Porcine
Sample Types: Whole Blood
Applications: ELISA Capture -
Beneficial effects of inhaled nitric oxide with intravenous steroid in an ischemia-reperfusion model involving aortic clamping
Authors: W Gozdzik, S Zielinski, M Zielinska, K Ratajczak, P Skrzypczak, S Rodziewicz, A Kübler, K Löfström, P Dziegiel, M Olbromski, B Adamik, S Ryniak, P Harbut, J Albert, C Frostell
Int J Immunopathol Pharmacol, 2018-01-01;32(0):3946320177514.
Species: Porcine
Sample Types: Plasma
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Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway
Authors: H Yan, KM Ajuwon
PLoS ONE, 2017-06-27;12(6):e0179586.
Species: Porcine
Sample Types: Cell Culture Supernates
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Altered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium
Authors: M Kern, D Günzel, JR Aschenbach, K Tedin, A Bondzio, U Lodemann
Mediators Inflamm., 2017-05-14;2017(0):2748192.
Species: Porcine
Sample Types: Cell Culture Supernates
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Septic porcine blood does not further activate coagulation during in vitro membrane oxygenation
Authors: Rolf Rossaint
Eur J Cardiothorac Surg, 2017-03-01;0(0):.
Species: Porcine
Sample Types: Plasma
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Inhibition of CD34+ cell migration by matrix metalloproteinase-2 during acute myocardial ischemia, counteracted by ischemic preconditioning
Authors: D Lukovic, K Zlabinger, A Gugerell, A Spannbauer, N Pavo, L Mandic, DT Weidenauer, S Kastl, C Kaun, A Posa, I Sabdyushev, J Winkler, M Gyöngyösi
F1000Res, 2016-11-22;5(0):2739.
Species: Porcine
Sample Types: Serum
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An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome
Authors: Angela J Westover
J Diabetes Res, 2016-10-13;2016(0):3486727.
Species: Porcine
Sample Types: Plasma
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Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide.
Authors: Qu X, Proll M, Neuhoff C, Zhang R, Cinar M, Hossain M, Tesfaye D, Grosse-Brinkhaus C, Salilew-Wondim D, Tholen E, Looft C, Holker M, Schellander K, Uddin M
PLoS ONE, 2015-03-20;10(3):e0121574.
Species: Porcine
Sample Types: Cell Culture Supernates
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Inhalation therapy with the synthetic TIP-like peptide AP318 attenuates pulmonary inflammation in a porcine sepsis model.
Authors: Hartmann E, Ziebart A, Thomas R, Liu T, Schad A, Tews M, Moosmann B, Kamuf J, Duenges B, Thal S, David M
BMC Pulm Med, 2015-02-07;15(0):7.
Species: Porcine
Sample Types: Plasma
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Soluble T cell immunoglobulin mucin domain 3 is shed from CD8+ T cells by the sheddase ADAM10, is increased in plasma during untreated HIV infection, and correlates with HIV disease progression.
Authors: Clayton K, Douglas-Vail M, Nur-ur Rahman A, Medcalf K, Xie I, Chew G, Tandon R, Lanteri M, Norris P, Deeks S, Ndhlovu L, Ostrowski M
J Virol, 2015-01-21;89(7):3723-36.
Species: Porcine
Sample Types: Plasma
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Low tidal volume pressure support versus controlled ventilation in early experimental sepsis in pigs.
Authors: Ziebart A, Hartmann E, Thomas R, Liu T, Duenges B, Schad A, Bodenstein M, Thal S, David M
Respir Res, 2014-09-06;15(0):101.
Species: Porcine
Sample Types: Plasma
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Risks Associated with High-Dose Lactobacillus rhamnosus in an Escherichia coli Model of Piglet Diarrhoea: Intestinal Microbiota and Immune Imbalances.
Authors: Li XQ, Zhu YH, Zhang HF, Yue Y, Cai ZX, Lu QP, Zhang L, Weng XG, Zhang FJ, Zhou D, Yang JC, Wang JF
PLoS ONE, 2012-07-27;7(7):e40666.
Species: Porcine
Sample Types: Serum
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Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig.
Authors: Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S
Mol. Ther., 2012-03-27;20(6):1204-11.
Species: Porcine
Sample Types: Plasma
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Efficacy of linezolid compared to vancomycin in an experimental model of pneumonia induced by methicillin-resistant Staphylococcus aureus in ventilated pigs.
Authors: Martinez-Olondris P, Rigol M, Soy D, Guerrero L, Agusti C, Quera MA, Li Bassi G, Esperatti M, Luque N, Liapikou M, Filella X, Marco F, de la Bellacasa JP, Torres A
Crit. Care Med., 2012-01-01;40(1):162-8.
Species: Porcine
Sample Types: Serum
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Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans.
Authors: Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE
J. Immunol., 2011-09-30;187(9):4913-9.
Species: Porcine
Sample Types: Plasma
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Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.
Authors: Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P
Crit. Care Med., 2010-06-01;38(6):1467-74.
Species: Porcine
Sample Types: Plasma
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Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine.
Authors: McILwain RB, Timpa JG, Kurundkar AR
Lab. Invest., 2009-11-09;90(1):128-39.
Species: Porcine
Sample Types: Plasma
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Cardiac Function and the Proinflammatory Cytokine Response After Recovery From Cardiac Arrest in Swine.
Authors: Niemann JT, Rosborough JP, Youngquist S, Shah AP, Lewis RJ, Phan QT, Filler SG
J. Interferon Cytokine Res., 2009-11-01;0(0):.
Species: Porcine
Sample Types: Plasma
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CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
Authors: Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE
FASEB J., 2009-10-19;24(3):712-22.
Species: Porcine
Sample Types: Plasma
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Autologous mesenchymal stem cells produce reverse remodelling in chronic ischaemic cardiomyopathy.
Authors: Schuleri KH, Feigenbaum GS, Centola M, Weiss ES, Zimmet JM, Turney J, Kellner J, Zviman MM, Hatzistergos KE, Detrick B, Conte JV, McNiece I, Steenbergen C, Lardo AC, Hare JM
Eur. Heart J., 2009-07-08;30(22):2722-32.
Species: Porcine
Sample Types: Serum
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Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues.
Authors: Wall R, Ross RP, Shanahan F, O'Mahony L, O'Mahony C, Coakley M, Hart O, Lawlor P, Quigley EM, Kiely B, Fitzgerald GF, Stanton C
Am. J. Clin. Nutr., 2009-04-08;89(5):1393-401.
Species: Porcine
Sample Types: Cell Culture Supernates
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Effect of linezolid compared with glycopeptides in methicillin-resistant Staphylococcus aureus severe pneumonia in piglets.
Authors: Luna CM, Bruno DA, Garcia-Morato J, Mann KC, Risso Patron J, Sagardia J, Absi R, Garcia Bottino M, Marchetti D, Famiglietti A, Baleztena M, Biancolini C
Chest, 2009-01-13;135(6):1564-71.
Species: Porcine
Sample Types: Serum
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Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
Authors: Thorgersen EB, Pharo A, Haverson K, Axelsen AK, Gaustad P, Kotwal GJ, Sfyroera G, Mollnes TE
Infect. Immun., 2008-12-01;77(2):725-32.
Species: Porcine
Sample Types: Whole Blood
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Inducible nitric oxide synthase expression and cardiomyocyte dysfunction during sustained moderate ischemia in pigs.
Authors: Heinzel FR, Gres P, Boengler K, Duschin A, Konietzka I, Rassaf T, Snedovskaya J, Meyer S, Skyschally A, Kelm M, Heusch G, Schulz R
Circ. Res., 2008-09-25;103(10):1120-7.
Species: Porcine
Sample Types: Cell Lysates
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Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.
Authors: Kruse R, Essen-Gustavsson B, Fossum C, Jensen-Waern M
Acta Vet. Scand., 2008-08-12;50(0):32.
Species: Porcine
Sample Types: Serum
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Effects of glucocorticoids in ventilated piglets with severe pneumonia.
Authors: Sibila O, Luna CM, Agusti C, Baquero S, Gando S, Patron JR, Morato JG, Absi R, Bassi N, Torres A
Eur. Respir. J., 2008-05-28;32(4):1037-46.
Species: Porcine
Sample Types: Serum
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Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
Authors: Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE
J. Immunol., 2008-05-01;180(9):6346-53.
Species: Porcine
Sample Types: Cell Culture Supernates
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Protective effect of Sivelestat in a porcine hepatectomy model prepared using an intermittent Pringle method.
Authors: Shimoda M, Iwasaki Y, Okada T, Sawada T, Kubota K
Eur. J. Pharmacol., 2008-02-29;587(1):248-52.
Species: Porcine
Sample Types: Serum
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Activated protein C downregulates p38 mitogen-activated protein kinase and improves clinical parameters in an in-vivo model of septic shock.
Authors: Nold MF, Nold-Petry CA, Fischer D, Richter B, Blaheta R, Pfeilschifter J, Muhl H, Schranz D, Veldman A
Thromb. Haemost., 2007-11-01;98(5):1118-26.
Species: Porcine
Sample Types: Plasma
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Involvement of both tumor necrosis factor-alpha-induced necrosis and p53-mediated caspase-dependent apoptosis in nephrotoxicity of cisplatin.
Authors: Yano T, Itoh Y, Matsuo M, Kawashiri T, Egashira N, Oishi R
Apoptosis, 2007-10-01;12(10):1901-9.
Species: Porcine
Sample Types: Cell Culture Supernates
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Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response.
Authors: Sibila O, Agusti C, Torres A, Baquero S, Gando S, Patron JR, Morato JG, Goffredo DH, Bassi N, Luna CM
Eur. Respir. J., 2007-09-05;30(6):1167-72.
Species: Porcine
Sample Types: Serum
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Experimental severe Pseudomonas aeruginosa pneumonia and antibiotic therapy in piglets receiving mechanical ventilation.
Authors: Luna CM, Baquero S, Gando S, Patron JR, Morato JG, Sibila O, Absi R, Famiglietti A, Vay CA, Von Stecher F, Agusti C, Torres A
Chest, 2007-08-01;132(2):523-31.
Species: Porcine
Sample Types: Serum
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The fibrin-derived peptide Bbeta15-42 is cardioprotective in a pig model of myocardial ischemia-reperfusion injury.
Authors: Roesner JP, Petzelbauer P, Koch A, Mersmann J, Zacharowski PA, Boehm O, Reingruber S, Pasteiner W, Mascher D, Wolzt M, Barthuber C, Noldge-Schomburg GE, Scheeren TW, Zacharowski K
Crit. Care Med., 2007-07-01;35(7):1730-5.
Species: Porcine
Sample Types: Plasma
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Bidirectional role of tumor necrosis factor-alpha in coronary microembolization: progressive contractile dysfunction versus delayed protection against infarction.
Authors: Skyschally A, Gres P, Hoffmann S, Haude M, Erbel R, Schulz R, Heusch G
Circ. Res., 2006-12-14;100(1):140-6.
Species: Porcine
Sample Types: Tissue Homogenates
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Dietary fatty acid composition rather than vitamin E supplementation influence ex vivo cytokine and eicosanoid response of porcine alveolar macrophages.
Authors: Moller S, Lauridsen C
Cytokine, 2006-08-17;35(1):6-12.
Species: Porcine
Sample Types: Cell Culture Supernates
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Role of oxidative stress in remodeling of the myocardial microcirculation in hypertension.
Authors: Zhu XY, Daghini E, Chade AR, Rodriguez-Porcel M, Napoli C, Lerman A, Lerman LO
Arterioscler. Thromb. Vasc. Biol., 2006-05-18;26(8):1746-52.
Species: Porcine
Sample Types: Plasma
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Proangiogenic effects of protease-activated receptor 2 are tumor necrosis factor-alpha and consecutively Tie2 dependent.
Authors: Zhu T, Sennlaub F, Beauchamp MH, Fan L, Joyal JS, Checchin D, Nim S, Lachapelle P, Sirinyan M, Hou X, Bossolasco M, Rivard GE, Heveker N, Chemtob S
Arterioscler. Thromb. Vasc. Biol., 2006-01-26;26(4):744-50.
Species: Porcine
Sample Types: Cell Culture Supernates
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Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide.
Authors: Changhua L, Jindong Y, Defa L, Lidan Z, Shiyan Q, Jianjun X
J. Nutr., 2005-02-01;135(2):239-44.
Species: Porcine
Sample Types: Serum
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Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination.
Authors: Royaee AR, Husmann RJ, Dawson HD, Calzada-Nova G, Schnitzlein WM, Zuckermann FA, Lunney JK
Vet. Immunol. Immunopathol., 2004-12-08;102(3):199-216.
Species: Porcine
Sample Types: Whole Cells
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The effect of warm humidified CO2 on the dissipation of residual gas following laparoscopy in piglets.
Authors: Glew PA, Campher MJ, Pearson K, Schofield JC, Davey AK
J Am Assoc Gynecol Laparosc, 2004-05-01;11(2):204-10.
Species: Porcine
Sample Types: Serum
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Extracellular ubiquitin inhibits the TNF-alpha response to endotoxin in peripheral blood mononuclear cells and regulates endotoxin hyporesponsiveness in critical illness.
Authors: Majetschak M, Krehmeier U, Bardenheuer M, Denz C, Quintel M, Voggenreiter G, Obertacke U
Blood, 2002-10-24;101(5):1882-90.
Species: Porcine
Sample Types: Plasma
FAQs
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Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
Reviews for Porcine TNF-alpha Quantikine ELISA Kit
Average Rating: 4.5 (Based on 2 Reviews)
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Rachel Dyer, the technical service specialist, helped on solving the problem of a previous kit (lot P202036). A higher background was detected and the old kits were replaced by a new one (lot P187024) that worked fine.