Proteome Profiler™ Antibody Array Kits utilize chemiluminescence for detection of multiple analytes in one sample. Many of our kits can also be adapted for near-infrared (NIR) fluorescence detection by replacing the HRP-conjugated Streptavidin provided in the kit with IRDye® 800CW Streptavidin. This modification is not applicable to array kits that use an HRP-conjugated detection antibody instead of Streptavidin-HRP, and it is not recommended for arrays with phospho-specific antibodies due to increased signal noise.
Both chemiluminescence and fluorescence based Proteome Profiler™ Antibody Array Kits can be quickly and accurately analyzed by FluorChem™ systems with AlphaView software.
Figure 1 Supernatants from human umbilical vein enthothelial cells (HUVEC) with or without TNF-alpha treatment were screened for changes in cytokine expression. Proteome Profiler™ Human XL Cytokine Array membranes (left; Catalog # ARY022B) were imaged for 10 minutes on a FluorChem™ R system, and AlphaView software was used to quantitate the change in cytokine and soluble receptor expression (right).
Learn more about how Proteome Profiler™ Antibody Arrays and FluorChem™ systems can work together to advance your research. Our App Note contains array protocol details, a description of instrument and software capabilities, and multiple data examples.
Watch our video to learn more about FluorChem™ systems. See how easy it is to generate high quality images from Western blot membranes.
Click this button to inquire about pricing for the various FluorChem™ imaging systems.
Articles using Proteome Profiler™ Antibody Arrays with FluorChem™ systems
A Novel Rat Model of Liver Regeneration: Possible Role of Cytokine Induced Neutrophil Chemoattractant-1 in Augmented Liver Regeneration. Dhar, D.K. et al. (2015) Ann. Surg. Innov. Res. 9:11.
- Profiled expression levels of multiple secreted cytokines at early and later stages of tissue regeneration
- Rat Cytokine Array Kit (Catalog # ARY008)
- FluorChem™ M system
Bacterium-Generated Nitric Oxide Hijacks Host Tumor Necrosis Factor Alpha Signaling and Modulates the Host Cell Cycle In Vitro. Mocca, B. and Wang, W. (2012) J. Bacteriol 194:4059.
PIWI-like Protein, HIWI2 is Aberrantly Expressed in Retinoblastoma Cells and Affects Cell Cycle Potentially Through OTX2. Sivagurunathan, S. et al. (2017) Cell Mol. Biol. Lett. 22:17.
- Profiled stem cell markers following HIWI2 knockdown in retinoblastoma cells
- Human Pluripotent Stem Cell Array Kit (Catalog # ARY010)
- FluorChem™ FC3 system