Rat CD34 Biotinylated Antibody

Catalog #: BAF4117 Datasheet / COA / SDS

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BAF4117

All CD34 Antibodies

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Rat CD34 Biotinylated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CD34 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant porcine CD34 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat CD34
Glu37-Ile293
Accession # NP_001100672

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat CD34

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

Rat CD34 is a 80-100 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352 amino acid (aa) protein with a 257 aa extracellular domain (ECD) (aa 35-291) and 74 aa cytoplasmic tail (aa 313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively.

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Alternate Names

CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1