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Rat IL-2 Quantikine ELISA Kit

Catalog #: R2000
21 Citations Datasheet / COA / SDS
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R2000
PR2000
SR2000
Rat IL-2 ELISA Standard Curve
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Citations (21)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Rat IL-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
15 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant rat IL-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Rat IL-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure rat IL-2 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat IL-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural rat IL-2 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural rat IL-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 130 478 1491 139 495 1550
Standard Deviation 4.2 10.5 31.9 13.8 30.8 85.4
CV% 3.2 2.2 2.1 9.9 6.2 5.5

Recovery

The recovery of rat IL-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=8) 110 93-120
EDTA Plasma (n=4) 102 90-117
Heparin Plasma (n=4) 100 92-116
Serum (n=14) 96 83-114

Linearity

To assess the linearity of the assay, four or more samples containing and/or spiked with various concentrations of rat IL-2 were diluted with Calibrator Diluent and then assayed.
Rat IL-2 ELISA Linearity

Scientific Data

N/A Rat IL-2 ELISA Standard Curve View Larger

Rat IL-2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-2

IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.

Long Name:
Interleukin 2
Entrez Gene IDs:
3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)
Alternate Names:
Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Rat IL-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Microglial synaptic pruning in the nucleus accumbens during adolescence sex-specifically influences splenic immune outcomes
    Authors: Kirkland, JM;Patel, I;Ardeshna, MS;Kopec, AM;
    bioRxiv : the preprint server for biology
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Silicified collagen scaffold induces semaphorin 3A secretion by sensory nerves to improve in-situ bone regeneration
    Authors: YX Ma, K Jiao, QQ Wan, J Li, MY Liu, ZB Zhang, W Qin, KY Wang, YZ Wang, FR Tay, LN Niu
    Bioactive materials, 2021-07-27;9(0):475-490.
    Species: Rat
    Sample Types: Plasma
  3. Non-alcoholic fatty liver disease is associated with bacterial translocation and a higher inflammation response in psoriatic patients
    Authors: I Belinchón-, P Bellot, D Romero-Pér, I Herraiz-Ro, F Marco, R Frances, JM Ramos-Rinc
    Scientific Reports, 2021-04-21;11(1):8593.
    Species: Rat
    Sample Types: Serum
  4. Nuclear Receptor Subfamily 4 Group A Member 1 Overexpression Prolongs Free Flap Allotransplant Graft Survival by Inducing T-Cell Anergy in the Rat
    Authors: B Xiao, Y Zhang, S Liu, J Ding, Z Yu, T Wang, B Song
    Transplant. Proc., 2020-09-09;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  5. Natural genetic variation in Stim1 creates stroke in the spontaneously hypertensive rat
    Authors: IS Dhande, SC Kneedler, Y Zhu, AS Joshi, MJ Hicks, SE Wenderfer, MC Braun, PA Doris
    Genes Immun., 2020-04-17;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  6. Melatonin synergized with cyclosporine A improves cardiac allograft survival by suppressing inflammation and apoptosis.
    Authors: Liu C, Hong T, Shao M, Chen Z, Wang C
    Mol Med Rep, 2014-07-15;10(3):1323-8.
    Species: Rat
    Sample Types: Serum
  7. Molecular identification of lyso-glycerophosphocholines as endogenous immunosuppressives in bovine and rat gonadal fluids.
    Authors: Foulds LM, Boysen RI, Crane M, Yang Y, Muir JA, Smith AI, de Kretser DM, Hearn MT, Hedger MP
    Biol. Reprod., 2008-05-28;79(3):525-36.
    Species: Rat
    Sample Types: Cell Culture Supernates
  8. Regulation of liver cytochrome P450 by activation of brain dopaminergic system: physiological and pharmacological implications.
    Authors: Wojcikowski J, Golembiowska K, Daniel WA
    Biochem. Pharmacol., 2008-05-03;76(2):258-67.
    Species: Rat
    Sample Types: Plasma
  9. Oridonin promotes CD4+/CD25+ Treg differentiation, modulates Th1/Th2 balance and induces HO-1 in rat splenic lymphocytes.
    Authors: Hu AP, Du JM, Li JY, Liu JW
    Inflamm. Res., 2008-04-01;57(4):163-70.
    Species: Rat
    Sample Types: Cell Culture Supernates
  10. Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
    Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
    J. Immunol., 2005-02-15;174(4):2327-35.
    Species: Rat
    Sample Types: Cell Culture Supernates
  11. Chlorpromazine and loxapine reduce interleukin-1beta and interleukin-2 release by rat mixed glial and microglial cell cultures.
    Authors: Labuzek K, Kowalski J, Gabryel B, Herman ZS
    Eur Neuropsychopharmacol, 2005-01-01;15(1):23-30.
    Species: Rat
    Sample Types: Cell Culture Supernates
  12. Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
    Authors: Schubert T, Horch RE
    Ann. Surg., 2004-08-01;240(2):340-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  13. Insulin attenuates the systemic inflammatory response in endotoxemic rats.
    Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
    Endocrinology, 2004-06-10;145(9):4084-93.
    Species: Rat
    Sample Types: Tissue Homogenates
  14. Gene transfer of tumor necrosis factor inhibitor improves the function of lung allografts.
    Authors: Tagawa T, Kozower BD, Kanaan SA, Daddi N, Muraoka M, Oka T, Ritter JH, Patterson GA
    J. Thorac. Cardiovasc. Surg., 2004-06-01;127(6):1558-63.
    Species: Rat
    Sample Types: Tissue Homogenates
  15. Oral (gavage), in utero and post-natal exposure of Sprague-Dawley rats to low doses of tributyltin chloride. Part II: effects on the immune system.
    Authors: Tryphonas H, Cooke G, Caldwell D, Bondy G, Parenteau M, Hayward S, Pulido O
    Food Chem. Toxicol., 2004-02-01;42(2):221-35.
    Species: Rat
    Sample Types: Serum
  16. Recipient intramuscular cotransfection of naked plasmid transforming growth factor beta1 and interleukin 10 ameliorates lung graft ischemia-reperfusion injury.
    Authors: D&amp;apos;Ovidio F
    J. Thorac. Cardiovasc. Surg., 2002-08-01;124(2):259-69.
    Species: Rat
    Sample Types: BALF
  17. Suppression of in vivo tumorigenicity of rat hepatoma cell line KDH-8 cells by soluble TGF-beta receptor type II.
    Authors: Zhao W, Kobayashi M, Ding W, Yuan L, Seth P, Cornain S, Wang J, Okada F, Hosokawa M
    Cancer Immunol. Immunother., 2002-06-25;51(7):381-8.
    Species: Rat
    Sample Types: Cell Culture Supernates
  18. Rat peripheral CD4+CD8+ T lymphocytes are partially immunocompetent thymus-derived cells that undergo post-thymic maturation to become functionally mature CD4+ T lymphocytes.
    Authors: Jimenez E, Sacedon R, Vicente A, Hernandez-Lopez C, Zapata AG, Varas A
    J. Immunol., 2002-05-15;168(10):5005-13.
    Species: Rat
    Sample Types: Cell Culture Supernates
  19. Melatonin fails to modulate immune parameters influenced by calorie restriction in aging Fischer 344 rats.
    Authors: Pahlavani MA, Vargas DA, Evans TR, Shu JH, Nelson JF
    Exp. Biol. Med. (Maywood), 2002-03-01;227(3):201-7.
    Species: Rat
    Sample Types: Cell Culture Supernates
  20. Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans.
    Authors: de Bernardis F, Santoni G, Boccanera M, Spreghini E, Adriani D, Morelli L, Cassone A
    Infect. Immun., 2000-06-01;68(6):3297-304.
    Species: Rat
    Sample Types: Vaginal Fluid
  21. Cloning and characterization of a retroviral plasmid, pCC1, for combination suicide gene therapy.
    Authors: Kuiper M, Sanches R, Gaken JA, Bignon YJ
    BioTechniques, 2000-03-01;28(3):572-4, 576.
    Species: Rat
    Sample Types: Cell Culture Supernates

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