Recombinant Canine IL-1 beta/IL-1F2 Protein Summary
Product Specifications
Ala114-Ser265
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
3747-CL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
3747-CL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 22% amino acid (aa) identity in dog. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The canine IL-1 beta cDNA encodes a 266 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature canine IL-1 beta shares 68% - 78% aa sequence identity with cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus IL-1 beta.
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- Accession # NP_001033060.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Citations for Recombinant Canine IL-1 beta/IL-1F2 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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MicroRNA-194 regulates parasitic load and IL-1?-dependent nitric oxide production in the peripheral blood mononuclear cells of dogs with leishmaniasis
Authors: Costa, SF;Soares, MF;Poleto Bragato, J;Dos Santos, MO;Rebech, GT;de Freitas, JH;de Lima, VMF;
PLoS neglected tropical diseases
Species: Canine
Sample Types: Transfected Whole Cells
Applications: Bioassay -
A DNA telomerase vaccine for canine cancer immunotherapy
Authors: J Thalmensi, E Pliquet, C Liard, G Chamel, C Kreuz, T Bestetti, M Escande, A Kostrzak, AS Pailhes-Ji, E Bourges, M Julithe, L Bourre, O Keravel, P Clayette, T Huet, S Wain-Hobso, P Langlade-D
Oncotarget, 2019-05-21;10(36):3361-3372.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay -
Dendritic cell vaccination plus low-dose doxorubicin for the treatment of spontaneous canine hemangiosarcoma
Authors: V Konduri, MM Halpert, YC Baig, R Coronado, JR Rodgers, JM Levitt, B Cerroni, S Piscoya, N Wilson, L DiBernardi, Z Omarbekov, L Seelhoff, V Ravi, L Douglass, WK Decker
Cancer Gene Ther., 2019-01-23;0(0):.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay -
Effect of interleukin-1? on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease
Authors: M Ogawa, H Osada, A Hasegawa, H Ohno, N Yanuma, K Sasaki, M Shimoda, J Shirai, H Kondo, K Ohmori
J. Vet. Intern. Med., 2018-03-23;0(0):.
Species: Canine
Sample Types: Whole Tissue
Applications: Bioassay
FAQs
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Does IL-1 beta induce NO in smooth muscle?
Yes it does, based on the literature.
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