Recombinant Cynomolgus Mesothelin (aa417-693) His Protein CF

Catalog #: 10764-MS Datasheet / COA / SDS

C-terminal

Catalog #	Availability	Size / Price	Qty
10764-MS-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Mesothelin (aa417-693) His Protein CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CA125/MUC16 (Catalog # 5609-MU) is immobilized at 2.00 ug/mL (100 µL/well), Recombinant Cynomolgus Monkey Mesothelin C-Terminal (aa 417-693) His-tag protein binds with an ED₅₀ of 0.700-4.20 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey Mesothelin protein
Asp417-Asp693, with a C-terminal 6-His tag

Accession #

XP_015298164.1

N-terminal Sequence
Analysis

Asp417

Predicted Molecular Mass

32 kDa

SDS-PAGE

35-45 kDa, under reducing conditions

Product Datasheets

10764-MS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10764-MS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CA125/MUC16 (5609-MU) is immobilized at 2.00 μg/mL (100 μg/well), Recombinant Cynomolgus Monkey Mesothelin C-Terminal (aa 417-693) His-tag (Catalog # 10764-MS) binds with an ED₅₀ of 0.700-4.20 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Cynomolgus Monkey Mesothelin (aa417-693) His-tag (Catalog # 10764-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-45 kDa.

Reconstitution Calculator

Background: Mesothelin

Mesothelin (MSLN), also known as CAK1 and ERC, is a glycosylated cell-surface antigen present on normal mesothelial cells and overexpressed in several human tumors (1, 2). The mesothelin gene encodes a ~70 kDa precursor protein that is cleaved at a dibasic proteolytic site into a 40-kDa membrane-bound protein termed MSLN and a 31-kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell (3, 4). Cleaved cynomolgus MSLN remains attached to the cell surface via a GPI linkage and shares 84% amino acid sequence identity with human MSLN. In human, alternate splicing generates additional MSLN isoforms that have either an eight amino acid insertion following Ser408 or a substituted C‑terminal region with no GPI anchor (3). Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas (1, 4). It is upregulated in mesotheliomas and a range of carcinomas and adenomas (5-7). Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression (8, 9). It is co-expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion (10). A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions (10-12).

References

Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
Muminova, Z.E. et al. (2004) BMC Cancer 4:19.
Hou, L.-Q. et al. (2008) Develop. Growth Differ. 50:531.
Ordonez, N.G. (2003) Mod. Pathol. 16:192.
Argani, P. et al. (2001) Clin. Cancer Res. 7:3862.
Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
Li, M. et al. (2008) Mol. Cancer Ther. 7:286.
Uehara, N. et al. (2008) Mol. Cancer Res. 6:186.
Rump, A. et al. (2004) J. Biol. Chem. 279:9190.
Ho, M. and M.O. Lively (2006) Cancer Epidemiol. Biomarkers Prev. 15:1751.
Robinson, B.W.S. et al. (2003) Lancet 362:1612.

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Alternate Names

CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein