Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein, CF

R&D Systems | Catalog # 11205-CD

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Key Product Details

  • R&D Systems CHO-derived Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein (11205-CD)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

EHH60309.1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all CD43 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey CD43 protein
Cynomolgus Monkey CD43
(Asp20-Thr258)
Accession # EHH60309.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp20

Predicted Molecular Mass

50 kDa

SDS-PAGE

106-118 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Cynomolgus Monkey CD-43 Fc Chimera (Catalog # 11205-CD) is immobilized at 1.00 μg/mL (100 μL/well), Recombinant Human Siglec-1 Protein (Catalog # 5197-SL) binds with an ED50 of 1.50-7.50 μg/mL.

Scientific Data Images for Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein, CF

Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein (Catalog # 11205-CD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 106-118 kDa and 210-240 kDa, respectively.

Formulation, Preparation, and Storage

11205-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD43

CD43, also known as Leukosialin and Sialophorin and Ly-48, is a type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells (1). Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific (2). CD43 can both induce and protect against apoptosis and can either promote or block cell adhesion (3). CD43 with altered glycosylations are expressed in cancers (4). The extracellular portion of cynomolgus monkey CD43 has a 78.2%, and 93.0% identity to human and rhesus monkeys, respectively.. CD43 induced cellular adhesion through the binding to molecules such as E-selectin (5, 6), galectin-1 and galectin-3 (7), siglec-1 (8), M-ficolin (9), integrins (10), cell surface nucleolin (11), and ICAM-1 (intercellular adhesion molecule type 1) (12). During cancer development, CD43 signalling induces the activation of -catenin, NF-B (13, 14), NFAT, and AP-1, which are prosurvival transcription factors that can promote tumorigenesis when deregulated (15, 16).

References

  1. Santamaría, M. et al. (1996) Cancer Res. 56:3526.
  2. Manjunath, N. et al. (1995) Nature 377:535.
  3. Brown, T.J. et al. (1996) J. Biol. Chem. 271:27686.
  4. Tuccillo, F.M. et al. (2014) BioMed Res International. https://doi.org/10.1155/2014/742831.
  5. Fuhlbrigge, R.C. et al. (2006) Blood 107:1421.
  6. Matsumoto, M. et al. (2005) J. Immuno. 175:8042.
  7. Yang, R.Y. et al. (2008) Expert Rev. Mol. Med. 10:e17.
  8. van den Berg, T.K. et al. (2001) J. Immuno. 166:3637.
  9. Moreno-Amaral, A.N. et al. (2012) J. Leukoc. Bio. 91:469.
  10. Sanchez-Mateos, P. et al. (1995) Blood, 86:2228.
  11. Hirano, K. et al. (2005) J. Biol. Chem. 280:39284.
  12. Rosenstein, Y. et al. (1991) Nature 354:233.
  13. Fiume, G. et al. (2013) PLoS ONE 8:e66087.
  14. Fiume, G. et al. (2012) Nucleic Acids Res. 40:3548.
  15. Andersson, C.C. et al. (2005) Biochem. J. 387:377.
  16. Santana, M.A. et al. (2000) J Biol. Chem. 275:31460.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Gene Symbol

SPN

UniProt

EHH60309.1

Additional CD43 Products

Product Documents for Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Cynomolgus Monkey CD43 Fc Chimera Protein, CF

For research use only

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