Recombinant Feline IL-2 (Cys146Ser) Protein

Catalog #: 1890-FL Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
1890-FL-020/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
1890-FL-020

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Feline IL-2 (Cys146Ser) Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED₅₀ for this effect is 0.02-0.12 ng/mL.

Source

E. coli-derived feline IL-2 protein
Ala21-Thr154 (Cys146Ser), with and without an N-terminal Met

Accession #

Q07885

N-terminal Sequence
Analysis

Met & Ala21

Predicted Molecular Mass

15.6 kDa

Product Datasheets

1890-FL (with carrier)

Product Datasheet

COA

SDS

1890-FL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1890-FL

Formulation	Lyophilized from a 0.2 μm filtered solution in Sodium Acetate with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1890-FL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Sodium Acetate.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-2

Interleukin-2 (IL-2) is a secreted, single chain alpha ‑helical polypeptide that has potent stimulatory activity for antigen-activated T cells. The feline IL-2 gene encodes a 154 amino acid (aa) precursor protein with a 20 aa signal peptide plus a 134 aa mature segment. There are suggestions that the mature protein may be O-glycosylated. At the aa sequence level, mature feline IL-2 is 78%, 82%, 60%, 64%, 62%, 75%, 62%, and 76% identical to mature human, canine, mouse, rat, cotton rat, porcine, goat, and equine IL-2, respectively. Mammalian cells known to express IL-2 include CD4⁺ and CD8⁺ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The biological activity of IL-2 is mediated by IL-2 receptor complexes consisting of three distinct subunits ( alpha, beta, gamma ) in two combinations. The high‑affinity signaling IL-2 receptor complex is a heterotrimer of the IL-2 receptor alpha, beta, gamma subunits. The intermediate signaling complex is a heterodimer of the IL-2 R beta  and gamma subunits. The non-ligand binding gamma subunit, referred to as the common gamma subunit ( gamma _c), is also a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-15. Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. On naïve CD8⁺ T cells, high IL-2 levels can induce cell proliferation with a bias towards cytotoxicity. In the presence of low levels of IL-2, CD8⁺ T cells preferentially undergo apoptosis with a bias towards cytokine secretion. IL-2 also seems to play a central role in the expansion and maintenance of CD4⁺ CD25⁺ regulatory T cells. This indicates IL-2 may be a key cytokine in the natural suppression of autoimmunity (1 - 9).

References

Cozzi, P.J. et al. (1993) Biochem. Biophys. Res. Commun. 194:1038.
Smith, K.A. (1992) Curr. Opin. Immunol. 4:271.
Conradt, H.S. et al. (1989) J. Biol. Chem. 264:17368.
Matesanz, F. et al. (1993) Immunogenetics 38:300.
Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27.
Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
Lin, J-X. and W.J. Leonard (1997) Cytokine Growth Factor Rev. 8:313.
Sad, S. and L. Krishnan (1999) J. Immunol. 163:2443.
Malek, T.R. (2003) J. Leukoc. Biol. 74:961.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF