Recombinant Human Annexin A3 Protein, CF

Catalog #: 9410-AN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9410-AN-050

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human Annexin A3 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

E. coli-derived human Annexin A3 protein
Ala2-Asp323, with a C-terminal 6-His tag

Accession #

P12429

N-terminal Sequence
Analysis

Ala2

Structure / Form

Predicted Molecular Mass

37 kDa

SDS-PAGE

36 kDa, reducing conditions

Product Datasheets

9410-AN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9410-AN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Annexin A3

Annexin A3 (ANXA3), also known as Annexin III and Lipocortin III (LPC3), is a 37 kDa member of the Annexin family. Annexins are calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexins consist of a unique N-terminal domain followed by a homologous C-terminal core domain containing the phospholipid-binding sites. The C-terminal domain is comprised of four 60‑70 aa annexin repeats which form a tightly packed disc known as the annexin domain. Members of the Annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways (1). Annexin A3 is up-regulated in microglia following stroke and in parenchymal hepatocytes in regenerating liver (2, 3). It can be up- or down‑regulated in various carcinomas (4). Alternate splicing generates an additional isoform with a 30 amino acid (aa) deletion near the N-terminus (5). Human Annexin A3 shares 89% and 85% aa sequence identity with mouse and rat Annexin A3, respectively.

References

Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.
Kessler, C., et al. (2007). Rom. J. Morphol. Embryol. 49:27.
Harashima, M. et al. (2008). J. Biochem. 143:537.
Wu, N., et al. (2013). Clin. Transl. Oncol. 15:106.
Bianchi, C. et al. (2010). Am. J. Pathol. 176:1660.

Entrez Gene IDs

306 (Human); 11745 (Mouse); 25291 (Rat)

Alternate Names

35-alpha calcimedin; Annexin A3; annexin A3,1,2-cyclic-inositol-phosphate phosphodiesterase; Annexin III; annexin-3; ANXA3; Calcimedin 35-alpha ; calcimedin 35-alpha; Lipocortin III; PAP-III; Placental anticoagulant protein III; placental anticoagulant protein III, calcimedin 35-alpha