Recombinant Human C1qTNF1 Protein, CF

Catalog #: 9268-TN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9268-TN-050

Recombinant Human C1qTNF1 Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human C1qTNF1 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human BAI3 is coated at 4 µg/mL, Recombinant Human C1qTNF1 (Catalog # 9268-TN) binds with an
ED₅₀ = 0.6-3.6 µg/mL.

Source

Mouse myeloma cell line, NS0-derived human CTRP1/C1qTNF1 protein
Arg26-Pro281, with a C-terminal 6-His tag

Accession #

Q9BXJ1

N-terminal Sequence
Analysis

Arg26

Predicted Molecular Mass

30 kDa

SDS-PAGE

33-41 kDa, reducing conditions

Product Datasheets

9268-TN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9268-TN

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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When Recombinant Human BAI3 is coated at 4 µg/mL, Recombinant Human C1qTNF1 (Catalog # 9268-TN) binds with an ED₅₀ of 0.6-3.6 µg/mL.

Reconstitution Calculator

Background: CTRP1/C1qTNF1

C1qTNF1 (CTRP1) is an approximately 35 kDa member of the C1q family of secreted proteins and plays a role in energy metabolism and inflammation (1, 2). C1qTNF1 contains a collagen-like region and one C1q-like domain (3). Mature human C1qTNF1 shares 80% aa sequence identity with mouse and rat C1qTNF1. Circulating levels of C1qTNF1 are elevated in obesity, hypertension, and diabetes but can be decreased in the serum of diet-induced obese mice (4-6). C1qTNF1 expression is up-regulated in atherosclerotic plaques or adipose tissue by oxidized LDL or inflammatory cytokines (3, 7, 8). In turn, it induces the expression of inflammatory cytokines (7, 9) and the up-reglation of adhesion proteins on vascular endothelial cells (8). Systemically administered C1qTNF1, in contrast, can limit tissue damage following myocardial infarction (9). In skeletal muscle, C1qTNF1 promotes fatty acid oxidation, energy expenditure, insulin sensitivity, and glucose uptake and glycolysis (6, 10). It also induces the proliferation of immature chondrocytes (11) and aldosterone synthesis in the adrenal cortex (4). R&D Systems in-house testing indicates that C1qTNF1 binds to BAI3, consistent with the reported interactions between BAI3 and C1qL proteins (12).

References

Grebrehiwet, B. et al. (2012) Front. Immunol. 5:3.
Seldin, M.M. et al. (2014) Rev. Endocr. Metab. Disord. 15:111.
Kim, K.-Y. et al. (2006) FEBS Lett. 580:3953.
Jeon, J.H. et al. (2008) FASEB J. 22:1502.
Xin, Y. et al. (2014) Endocr. J. 61:841.
Peterson, J. M. et al. (2012) J. Biol. Chem. 287:1576.
Wang, X.Q. et al. (2016) Atherosclerosis 250:38.
Lu, L. et al. (2016) Eur. Heart J. 37:1762.
Yuasa, D. et al. (2016) FASEB J. 30:1065.
Han, S. et al. (2016) J. Nutr. Biochem. 27:43.
Akiyama, H. et al. (2013) Mol. Cell. Endocrinol. 369:63.
Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.

Long Name

C1q And Tumor Necrosis Factor Related Protein 1

Entrez Gene IDs

114897 (Human); 56745 (Mouse); 303701 (Rat)

Alternate Names

C1q and tumor necrosis factor related protein 1; C1qTNF1; CTRP1; CTRP1G protein-coupled receptor-interacting protein; FLJ90694; G protein coupled receptor interacting protein; GIPcomplement C1q tumor necrosis factor-related protein 1; ZSIG37