Recombinant Human CEACAM-5/CD66e Fc Chimera Protein, CF

Catalog #: 10449-CM Datasheet / COA / SDS

HEK293 Expressed Protein

Catalog #	Availability	Size / Price	Qty
10449-CM-050

Recombinant Human CEACAM-5/CD66e Fc Chimera Protein Binding Activity

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CEACAM-5/CD66e Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CEACAM-5/CD66e Fc Chimera (Catalog # 10449-CM) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human Galectin-4 (Catalog # 1227-GA) binds with an ED₅₀ of 10-90 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human CEACAM-5/CD66e protein

Human CEACAM-5/CD66e (Lys35-Ala685) Accession # NP_004354.3	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

NP_004354.3

N-terminal Sequence
Analysis

Lys35

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

96 kDa

SDS-PAGE

135-175 kDa, under reducing conditions.

Product Datasheets

10449-CM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10449-CM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human CEACAM-5/CD66e Fc Chimera (10449-CM) is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Human Galectin-4 (1227-GA) binds with an ED₅₀ of 10-90 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human CEACAM-5/CD66e Fc Chimera Protein (Catalog # 10449-CM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 135-175 kDa and 270-330 kDa, respectively.

Reconstitution Calculator

Background: CEACAM-5/CD66e

CEACAM-5, also known as CEA, CD66e and Psg30, belongs to the large family of CEACAM and pregnancy specific glycoproteins. CEACAM family members are highly glycosylated with varying arrangements of IgV-like and IgC-like regions in their extracellular domains (ECD) and can be expressed as transmembrane, glycophosphatidyl inositol (GPI) linked or soluble proteins (1-3). CEACAM-5 consists of an N-terminal Ig-like V-set domain followed by six Ig-like C2-set domains and a GPI anchor (2, 4, 5). While the mature ECD of human CEACAM-5 shares 26% amino acid identity with mouse CEACAM-5, it remains unclear if these molecules are direct orthologs (6). CEACAM-5, expressed primarily by epithelial cells, functions as a calcium-independent adhesion molecule through homophilic and heterophilic interactions with CEACAM-1 (1, 7). CEACAM-5 is restricted to the apical face of intestinal epithelial cells in the adult but is more diffuse during embryonic development and in tumors (8). This is consistent with a role in the development and maintenance of epithelial architecture. CEACAM-5 is up-regulated in a wide variety of human tumors, promoting tumor cell migration, invasion, adhesion, and metastasis, and has been used as a cancer marker (8, 9). It also contributes to tumor formation by maintaining cellular proliferation in the presence of differentiation stimuli, and by blocking apoptosis following loss of ECM anchorage (anoikis) (10, 11). The GPI anchoring of CEACAM-5 can be released by GPI-PLD, resulting in a soluble molecule that also promotes tumor metastasis (12). Cell surface expression of CEACAM-5 on tumor cells prevents the adhesion of CEACAM-1 expressing NK cells and provides protection from NK-mediated lysis (8). CEACAM-5 was shown to bind galectin-4 and by surface plasmon resonance and coimmunoprecipitated with galectin-4 in human colon adenocarcinoma LS174T cell lysates (13). CEACAM-5 also binds a subset of Neisseria opacity proteins (Opa) and E. coli adhesion proteins (14, 15). These interactions trigger clustering of the lipid raft-localized CEACAM-5 to sites of pathogen contact (15, 16).

References

Zebhauser, R. et al. (2005) Genomics 86:566.
Hammarstrom, S. (1999) Semin. Cancer Biol. 9:67.
Chan, et al. (2007) Curr Oncol. 14:70.
Schrewe, H. et al. (1990) Mol. Cell. Biol. 10:2738.
Hefta, S.A. et al. (1988) Proc. Natl. Acad. Sci. 85:4648.
Hance, K.W. et al. Mutat Res. (2005) 576:132.
Stern, N. et al. (2005) J. Immunol. 174:6692.
Beauchemin N. et al. (2013) Cancer Metastasis Rev. 32:3.
Blumenthal, R.D. et al. (2005) Cancer Res. 65:8809.
Screaton, R.A. et al. (1997) J. Cell Biol. 137:939.
Ordonez, C. et al. (2000) Cancer Res. 60:3419.
Yamamoto, Y. et al. (2005) Biochem. Biophys. Res. Commun. 333:223.
Ideo, H. et al. (2005) J Biol Chem. 280:4730.
Martin, J.N. et al. (2016) Biochemistry. 55:4286.
Berger, C.N. et al. (2004) Mol. Microbiol. 52:963.
Tchoupa, A.K. et al. (2014) Cell Commun Signal. 12:27.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5

Entrez Gene IDs

1048 (Human); 73250 (Mouse); 102128791 (Cynomolgus Monkey)

Alternate Names

Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100