Recombinant Human CEACAM-6/CD66c Fc Avi-tag Protein, CF

Catalog #: AVI10928 Datasheet / COA / SDS

Biotinylated

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AVI10928-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human CEACAM-6/CD66c Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human CEACAM-8/CD66b Protein (Catalog # 9639-CM) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human CEACAM‑6/CD66c Fc Chimera Avi-tag (Catalog # AVI10928) binds with an ED₅₀ of 0.20-2.00 µg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human CEACAM-6/CD66c protein

Human CEACAM‑6/CD66c (Lys35-Gly320) Accession # NP_002474.4	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

NP_002474.4

N-terminal Sequence
Analysis

Lys35

Structure / Form

Disulfide-linked homodimer

Biotinylated via Avi-tag

Predicted Molecular Mass

60 kDa

SDS-PAGE

85-100 kDa, under reducing conditions.

Product Datasheets

AVI10928

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10928

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity

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When Recombinant Human CEACAM-8/CD66b Protein (9639-CM) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human CEACAM‑6/CD66c Fc Chimera Avi-tag (Catalog # AVI10928) binds with an ED₅₀ of 0.200-2.00 µg/mL

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human CEACAM‑6/CD66c Fc Chimera Avi-tag Protein (Catalog # AVI10928) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-100 kDa and 170-200 kDa, respectively.

Reconstitution Calculator

Background: CEACAM-6/CD66c

Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM-6), previously called nonspecific cross-reacting antigen (NCA) or CD66c, is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily primarily found in mammals. CEACAMs are transmembrane proteins used by several bacterial pathogens to bind and invade host cells (1-3). Mature, human CEACAM-6 is a GPI-linked membrane protein that contains an extracellular domain (ECD) with one N-terminal V-type Ig-like domain and two C2-type Ig-like domains (1, 3). The GPI membrane anchor is attached at the C-terminus following cleavage of the propeptide (4). The mature ECD of human CEACAM-6 shares 46% amino acid sequence identity to rat CEACAM-6 and is absent in mouse. CEACAM-6 is an intercellular adhesion molecule and forms both homotypic and heterotypic bonds with CEACAM-1, -5 and -8 through interaction of the V-type Ig-like domains (5, 6). CEACAM-6 is expressed by granulocytes and their precursors and granulocyte activation enhances surface expression by mobilizing CEACAM-6 from storage in azurophilic granules (7). CEACAM-6 is also expressed in epithelia of various organs and is upregulated in pancreatic and colon adenocarcinomas and hyperplastic polyps (7, 8). CEACAM-6 often shows aberrant expression in acute lymphocytic leukemias, and over-expression confers resistance to adhesion-related apoptosis (anoikis) in tumor cells (9‑11). CEACAM-6 expression is elevated in many solid tumors including breast, pancreatic, colonic and non-small-cell lung carcinoma, and is a biomarker or potential therapeutic for other carcinomas (12, 13). CEACAM-6 has been identified as the glycoprotein receptor for influenza virus and plays a role in virus entry (14). Our Avi-tag Biotinylated CEACAM-6 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Tchoupa, A. et al. (2014) J Cell Commun Signal 12:27.
Hauck, C.R. et al. (2006) Eur J Cell Biol. 85:235.
Kuespert, K. et al. (2006) Curr Opin Cell Biol 18:565.
Tawaragi, Y. et al. (1988) Biochem. Biophys. Res. Comm. 150:89.
Bonsor, D.A. et al. (2015) PNAS 112:13561.
Kuroki, M. et al. (2001) J. Leukoc. Biol. 70:543.
Ducker, T.P. and K.M. Skubitz (1992) J. Leukoc. Biol. 52:11.
Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
Duxbury, M.S. et al. (2004) J. Biol. Chem. 279:23176.
Ilantzis, C. et al. (2002) Neoplasia 4:151.
Kalina, T. et al. (2005) BMC Cancer 5:38.
Blumenthal, R.D. et al. (2007) BMC Cancer 7:2.
Ru, G.Q. et al. (2017) Oncotarget. 8:83673.
Rahman, S.K. et al. (2021) Viruses 13:726.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 6

Entrez Gene IDs

4680 (Human); 107126399 (Cynomolgus Monkey)

Alternate Names

carcinoembryonic antigen-related cell adhesion molecule 6 (non-specific crossreacting antigen); carcinoembryonic antigen-related cell adhesion molecule 6; CD66c antigen; CD66c; CEACAM6; CEACAM-6; CEAL; NCA; NCACEAL; Non-specific crossreacting antigen; Normal cross-reacting antigen